Development of a radiolabeled β-human chorionic gonadotropin

Jalilian, Amir; Khoshdel, Mohammad; Garousi, Javad; Yousefnia, Hassan; Hosseini, Mohammad; Rajabifar, Saeed; Sardari, Daryoush

doi:10.2478/v10007-009-0035-6

Abstract

β-Human chorionic gonadotropin (β-hCG) was successively labeled with [⁶⁷Ga]-gallium chloride after conjugation with freshly prepared diethylenetriaminepentaacetic acid dianhydride (ccDTPA). After solid phase purification of the radiolabeled hormone, high performance liquid chromatography showed radiochemical purity higher than 95 % under optimized conditions (specific activity = 22-23 TBq mM^-1, labeling efficiency 80 %). Preliminary in vivo studies (ID g^-1, %) in male wild-type rats showed marked gonadal uptake of the tracer after 240 minutes in agreement with the biodistribution studies and reported β-hCG receptors. Target to blood ratios were 5.1 and 15.2 after 3 and 24 hours, respectively, while target to muscle ratios were 35 and 40 after 3 and 24 hours, respectively.

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Development of a radiolabeled β-human chorionic gonadotropin

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