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The Impact of Cross-Clamping of the Portal Triad on the Morphology of the Small Intestine Cover

The Impact of Cross-Clamping of the Portal Triad on the Morphology of the Small Intestine

EABR. Experimental and Applied Biomedical Research
Volume 26 (2025): Issue 1 (March 2025)
By: ,  ,  ,  ,  ,  ,  ,   and    
Open Access
|Dec 2022
Figures & tables

Figures & Tables

Table 1.

Diameter of the links of the microvasculature of the small intestine of experimental rats with clamping of the PT for 6 minutes, depending on the observation time (M ± SD) (μm)

Parts of microcirculatory systemControlObservation time
3 hours6 hours12 hours24 hours (1 day)72 hours (3 days)168 hours (7 days)
Arterioles34±439,4±3,240±3,1*39,8±2,838,4±2,735±2,634,5±2,9
Pre-capillaries15±218,7±1,719,5±2,5*20±2,4*20±2,2*18±2,117±2,0
Capillaries5,4±18,3±0,7*8,7±0,6*8,9±0,5*8,8±0,6*6,7±0,56±0,9
Post-capillaries18±227±2,1*28±1,7*29±2,0*28,5±1,8*23±1,9*21,5±1,8
Venules40±3,560±3,9*61±3,4*60±3,6*58±3,4*50±3,2*45±3,4

* - p < 0.05 - reliability of difference with control

Table 2.

Diameters of the links of the microvasculature of the small intestine of experimental rats with clamping of the PT for 12 minutes, depending on the observation time (M ± SD) (μm)

Parts of microcirculatory systemControlObservation time
3 hours6 hours12 hours24 hours (1 day)72 hours (3 days)168 hours (7 days)
Arterioles34±42±3,140,6±2,741±2,840±2,738,3±2,36±2,7
Pre-capillaries15±224,5±2,5*20±2,2*21±2,1*22±1,9*19,5±1,9*17,6±1,6
Capillaries5,4±110,7±0,7*9,8±0,5*9,9±0,6*9,0±1,0*8,6±0,7*8,2±0,6*
Post-capillaries18±232±1,8*30±1,8*32±1,7*33±1,9*27,6±1,7*26,8±1,5*
Venules40±3,566±3,3*58±3,4*62±3,2*61±3,4*56±3,254±3,1*

* - p < 0.05 - reliability of difference with control

Table 3.

Diameters of the links of the microvasculature of the small intestine of experimental rats with clamping of the PT for 24 minutes, depending on the observation time (M ± SD) (μm)

Parts of micro-circulatory systemControlObservation time
3 hours6 hours12 hours24 hours (1 day)72 hours (3 days)168 hours (7 days)
Arterioles34±446±3,3*44±2,9*45±3,0*41±2,6*40±2,5*39±2,4
Pre-capillaries15±228±2,7*24,4±2,6*22±2,3*21±2,0*22±1,8*21±1,9*
Capillaries5,4±112,6±0,6*11,2±0,8*10±0,7*9,5±0,5*9,0±0,7*8,8±0,6*
Post-capillaries18±235±1,9*32±1,7*31±1,6*31±1,8*32±2,0*30±1,8*
Venules40±3,572±3,4*68±3,5*66±3,4*62±3,3*61±3,2*59±2,9*

* - p < 0.05 - reliability of difference with control

Fig. 1.

Histological picture of small intestine microcirculation in experimental rats during PT clamping for 6, 12 and 24 minutes, depending on the observation time (A, G, M - after 3 hours; B, H, N - after 6 hours; C, I, O - after 12 hours; D, J, P-1 day; E, K, Q - 3 days; F, L, R-7 days). The silver nitrate staining protocol. Magnification ×200, scale bars (white) 100 μm.

Fig. 2.

Histological picture of small intestine microcirculation in experimental rats during PT clamping for 6,12 and 24 minutes, depending on the observation time (A1, G1, M1 - after 3 hours; B1, H1, N1 - after 6 hours; C1, I1, O1 - after 12 hours; D1, J1, P1 - 1 day; E1, K1, Q1 - 3 days; F1, L1, R1 - 7 days). Hematoxylin-eosin. Magnification ×100, scale bars (black) 100 μm.

Table 4.

Mortality of experimental animals in different study groups

GroupsExperimental modelOperated animalsLethality of animals (L / N)Total %
3h6 h12 h24 h72 h168 h
IControl10/94-------
IIClamping PT for 6 min28/94-/4-/4-/5-/5-/5-/5-
IIIClamping PT for 12 min28/94-/4-/4-/51/51/52/54/14.3
IVClamping PT for 24 min28/941/41/42/52/53/53/512/42.8

[i] L-lethality in each time interval;

[ii] N is the number of animals participating in the experiment in a certain time interval

DOI: https://doi.org/10.2478/sjecr-2021-0083 | Journal eISSN: 2956-2090 (formerly 2335-075X, 2956-0454) | Journal ISSN: 2956-0454 (formerly 1820-8665)
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Language: English
Page range: 75 - 84
Submitted on: May 6, 2021
Accepted on: Nov 19, 2021
Published on: Dec 31, 2022
Published by: University of Kragujevac, Faculty of Medical Sciences
In partnership with: Paradigm Publishing Services
Keywords:
Clamping of the portal triad,
morphological changes,
small intestine,
ischemic-reperfusion injury
Related subjects:
Medicine,
Clinical medicine,
Clinical medicine, other

© 2022 Ydyrys Almabayev, Bagdat Salimgereeva, Marat Kamyspayev, Maral Yergazina, Zhannym Yermentayeva, Makhabbat Zhelderbayeva, Raikhan Dzhunusova, Ildar Fakhradiyev, Shynar Tanabayeva, published by University of Kragujevac, Faculty of Medical Sciences
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Volume 26 (2025): Issue 1 (March 2025)
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