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Antitumor Effect of the Synthesized Chalcone Analogues on HeLa Cell Line Cover

Antitumor Effect of the Synthesized Chalcone Analogues on HeLa Cell Line

EABR. Experimental and Applied Biomedical Research
Volume 26 (2025): Issue 1 (March 2025)
By: Marija Anđelković,  Ivana Nikolić,  Jovan Luković,  Marina Mitrović,  Ivanka Zelen,  Jovana Muškinja,  Zoran Ratković,  Suzana Popović,  Sanja Stanković and  Marijana Stanojević Pirković  
Open Access
|Apr 2022

Figures & Tables

Figure 1.

Structures of tested chalcone analogues.
Structures of tested chalcone analogues.

Figure 2.

The effects of the various concentrations of new chalcone analogues on viability of HeLa cancer cells after 24 h treatment (A) and 48 h (B). Viability was quantified by MTT assay. Results are mean ± SD of three experiments (p<0.05)
The effects of the various concentrations of new chalcone analogues on viability of HeLa cancer cells after 24 h treatment (A) and 48 h (B). Viability was quantified by MTT assay. Results are mean ± SD of three experiments (p<0.05)

Figure 3.

The effects of new synthesized chalcone analogues on cytotoxicity of HeLa and MRC-5 cells after 24 h (A) and 48 h (B) in comparison to DHZ and cisplatin treatment. Results are mean ± SD of three experiments (p<0.05).
The effects of new synthesized chalcone analogues on cytotoxicity of HeLa and MRC-5 cells after 24 h (A) and 48 h (B) in comparison to DHZ and cisplatin treatment. Results are mean ± SD of three experiments (p<0.05).

Figure 4.

Morphology of the HeLa cells after 48 h treatment with investigated substances. Equal number of HeLa cells were plated in 24 well plates and allowed to attach for 24 h. Cells were exposed to vehicle (VEH)-containing complete media and different concentrations of CH, DHZ and cisplatin during 48 h period and morphology of the cells was analysed on microscope.
Morphology of the HeLa cells after 48 h treatment with investigated substances. Equal number of HeLa cells were plated in 24 well plates and allowed to attach for 24 h. Cells were exposed to vehicle (VEH)-containing complete media and different concentrations of CH, DHZ and cisplatin during 48 h period and morphology of the cells was analysed on microscope.

Figure 5.

Effects of CH analogues on apoptosis and autophagy in HeLa cells. Flow cytometry analysis of Annexin V-FITC/7-AAD stained HeLa cells after 48 h of the treatment with CH, DHZ, cisplatin and co treatment with CQ.
Effects of CH analogues on apoptosis and autophagy in HeLa cells. Flow cytometry analysis of Annexin V-FITC/7-AAD stained HeLa cells after 48 h of the treatment with CH, DHZ, cisplatin and co treatment with CQ.

Figure 6.

Expression of apoptosis-related proteins in untreated (control) and treated HeLa cells presented by histograms (Fig. 6 A and B) and bar chart (Fig 6. C). The mean fluorescence intensity (MFI) of Bax (A) and Bcl-2 staining (B) are indicated on histograms. Bar chart (C) showing Bcl-2/Bax ratio in untreated and treated cells.
Expression of apoptosis-related proteins in untreated (control) and treated HeLa cells presented by histograms (Fig. 6 A and B) and bar chart (Fig 6. C). The mean fluorescence intensity (MFI) of Bax (A) and Bcl-2 staining (B) are indicated on histograms. Bar chart (C) showing Bcl-2/Bax ratio in untreated and treated cells.

IC50 values (μM) of chalcone analogues (CH), cisplatin and dehydrozingerone (DHZ) after 24 and 48 h treatment of HeLa cancer cells and noncancerous MRC-5 cell_

HeLa IC50CH1CH2CH3cisPtDHZ
24 h3.97 ± 1.454.11 ± 1.466.18 ± 1.729.70 ± 1.223.61 ± 2.16
48 h3.67 ± 1.173.5 ±1.11.69 ± 0.683.8 ± 0.42.41 ± 0.57
MRC-5 IC50CH1CH2CH3cisPtDHZ
24 h> 200> 200> 200< 150< 300
48 h> 200> 200> 200< 40< 300
DOI: https://doi.org/10.2478/sjecr-2021-0065 | Journal eISSN: 2956-2090 | Journal ISSN: 2956-0454
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Language: English
Page range: 15 - 22
Submitted on: Oct 30, 2021
Accepted on: Dec 19, 2021
Published on: Apr 18, 2022
Published by: University of Kragujevac, Faculty of Medical Sciences
In partnership with: Paradigm Publishing Services
Publication frequency: 4 issues per year
Keywords:
Apoptosis,
cervical cancer,
chalcones,
cytotoxicity,
HeLa
Related subjects:
Medicine,
Clinical medicine,
Clinical medicine, other

© 2022 Marija Anđelković, Ivana Nikolić, Jovan Luković, Marina Mitrović, Ivanka Zelen, Jovana Muškinja, Zoran Ratković, Suzana Popović, Sanja Stanković, Marijana Stanojević Pirković, published by University of Kragujevac, Faculty of Medical Sciences
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

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