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Impact of smoking on microRNAs in significant coronary artery disease Cover

Impact of smoking on microRNAs in significant coronary artery disease

Romanian Journal of Internal Medicine
Volume 63 (2025): Issue 1 (March 2025)
By: Shokoufeh Hajsadeghi,  Aida Iranpour,  Shayan Mirshafiee,  Reza Nekouian,  Maryam Mollababaei,  Hamed Motevalli,  Seyyed Amir Yasin Ahmadi and  Mohammad Sedigh Dakkali  
Open Access
|Mar 2025

Figures & Tables

Figure 1.

Coefficient plots showing FC and 95% CI (H0: FC =1, reference: S-A-) for the microRNAs having significant FC; B0: intercept of the models.
Coefficient plots showing FC and 95% CI (H0: FC =1, reference: S-A-) for the microRNAs having significant FC; B0: intercept of the models.

Figure 2.

Heat map for simultaneous clustering of microRNAs and participants on the basis of -∆CT (i.e. red color: up-regulation).
Heat map for simultaneous clustering of microRNAs and participants on the basis of -∆CT (i.e. red color: up-regulation).

Figure 3.

Heat map for simultaneous clustering of microRNAs and participants after removal of microRNAs with non-significant FCs on the basis of -∆CT (i.e. red color: up-regulation).
Heat map for simultaneous clustering of microRNAs and participants after removal of microRNAs with non-significant FCs on the basis of -∆CT (i.e. red color: up-regulation).

Figure 4.

Mir-34a expression may be affected by smoking, whereas mir-126-3p expression may be affected by atherosclerosis. The significant down-regulation of mir-199 for the interaction of smoking dose and severity of atherosclerosis was a notable finding showing the harmful consequence of this interaction. Further studies are needed for this micro-RNA.
Mir-34a expression may be affected by smoking, whereas mir-126-3p expression may be affected by atherosclerosis. The significant down-regulation of mir-199 for the interaction of smoking dose and severity of atherosclerosis was a notable finding showing the harmful consequence of this interaction. Further studies are needed for this micro-RNA.

Sensitivity power analysis for comparison of two means

MicroRNASD (∆CT)Cohen’s dDetectable ∆CTDetectable FC
Mir-34a2.921.063.108.55
Mir-34c3.191.063.3810.42
Mir-1013.981.064.2218.62
Mir-1242.381.062.525.75
Mir-126-3p4.961.065.2638.26
Mir-126-5p4.041.064.2819.46
Mir-1993.201.063.3910.50
Mir-2234.031.064.2719.32

Gene expression study in among groups using general linear model

MicroRNAGroupFC (95% CI)P valueR-squareANOVA P value
Mir-34aS-A-Reference 0.1530.031*
S+A-0.13 (0.03–0.56)0.007*
S-A+0.58 (0.13–2.52)0.461
S+A+0.23 (0.06–0.91)0.036*
Mir-34cS-A-Reference 0.0430.507
S+A-0.95 (0.17–5.17)0.952
S-A+2.31 (0.41–13.06)0.336
S+A+2.52 (0.51–12.58)0.253
Mir-101S-A-Reference 0.1200.078#
S+A-0.2 (0.03–1.44)0.107#
S-A+2.87 (0.37–21.99)0.305
S+A+0.42 (0.06–2.76)0.358
Mir-124S-A-Reference 0.1150.094#
S+A-0.79 (0.23–2.64)0.694
S-A+2.25 (0.65–7.76)0.195#
S+A+0.47 (0.15–1.49)0.196#
Mir-126-3pS-A-Reference 0.1290.064#
S+A-1.36 (0.11–16.76)0.806
S-A+0.05 (<0.01–0.67)0.024*
S+A+0.71 (0.07–7.65)0.772
Mir-126-5pS-A-Reference 0.1050.122
S+A-3.32 (0.42–26.31)0.250
S-A+0.27 (0.03–2.27)0.224
S+A+2.07 (0.29–14.74)0.461
Mir-199S-A-Reference 0.1460.040*
S+A-9.38 (1.89–46.49)0.007*
S-A+2.97 (0.58–15.26)0.187#
S+A+1.53 (0.34–6.99)0.575
Mir-223S-A-Reference 0.0290.666
S+A-0.82 (0.09–7.04)0.852
S-A+1.88 (0.21–16.98)0.567
S+A+2.61 (0.34–20.12)0.350

Interaction of atherosclerosis and pack-years of smoking on the expression of microRNA using general linear model

MicroRNAPredictorFC (95% CI)P valueR-squareANOVA P value
Mir-34aNumber vessels0.52 (0.36–1.11)0.111#0.1150.089#
Pack-year0.90 (0.87–0.99)0.018*
Interaction1.05 (0.99–1.08)0.131#
Mir-199Number vessels2.37 (1.01–3.28)0.046*0.1920.011*
Pack-year1.16 (1.04–1.19)0.002*
Interaction0.91 (0.90–0.98)0.006*
DOI: https://doi.org/10.2478/rjim-2024-0031 | Journal eISSN: 2501-062X | Journal ISSN: 1220-4749
Journal RSS Feed
Language: English
Page range: 49 - 59
Submitted on: Sep 5, 2024
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Published on: Mar 31, 2025
Published by: N.G. Lupu Internal Medicine Foundation
In partnership with: Paradigm Publishing Services
Publication frequency: 4 issues per year
Keywords:
coronary artery disease,
gene expression,
genetic epidemiology,
personalized medicine,
atherosclerosis,
smoking
Related subjects:
Medicine,
Clinical medicine,
Internal medicine,
Internal medicine, other,
Cardiology,
Gastroenterology,
Rheumatology

© 2025 Shokoufeh Hajsadeghi, Aida Iranpour, Shayan Mirshafiee, Reza Nekouian, Maryam Mollababaei, Hamed Motevalli, Seyyed Amir Yasin Ahmadi, Mohammad Sedigh Dakkali, published by N.G. Lupu Internal Medicine Foundation
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

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