Figure 1
Figure 2
Figure 3
Figure 4
Clinical and histopathologic parameters in relation to morphological diagnostic categories
| Parameter, n (%) | All | Nevus | Melanoma | Melanoma with nevus | P -value |
|---|---|---|---|---|---|
| N (%) | 90 | 26 (29) | 19 (21) | 45 (50) | |
| Age of the patients (years) (median; range) | 48; 21-89 | 38; 22-53 | 60; 21-89 | 51; 26-85 | < 0.001 |
| Maximum diameter available for 81 lesions | 9; 2-29 | 7; 2-14 | 8; 3-29 | 11; 4-25 | 0.014 |
| Location | 0.076 | ||||
| Back | 46 (51) | 9 (35) | 9 (47) | 28 (62) | |
| Other | 44 (49) | 17 (65) | 10 (53) | 17 (38) | |
| Extension along eccrine ducts no eccrine ducts were identified within or adjacent to sclerotic dermal component in 13 lesions | 49/77 (64) | 5/21 (24) | 14/17 (82) | 30/39 (77) | < 0.001 |
| Evidence of regression | 29 (32) | 1 (4) | 12 (63) | 16 (36) | / |
| Lamellar sclerosis | 8 (9) | 6 (23) | 0 | 2 (4) | / |
| Marked inflammation in the sclerosis | 27 (30) | 2 (8) | 6 (32) | 19 (42) | / |
| Maturation in the sclerosis | 50 (56) | 25 (96) | 8 (42) | 17 (38) | / |
| Dermal mitoses present | 18 (20) | 1 (4) | 4 (21) | 13 (29) | / |
| Prominent nucleoli in the sclerosis | 52 (58) | 5 (19) | 16 (84) | 31 (69) | / |
| Intraepidermal component adjacent to the sclerosis | / | ||||
| Not atypical | 18 (20) | 17 (65) | 1 (5) | 0 | |
| Atypical | 17 (19) | 9 (35) | 1 (5) | 7 (16) | |
| Melanoma in situ | 55 (61) | 0 | 17 (89) | 38 (84) | |
| Severe cytological atypia in the epidermis above the sclerosis | 68 (76) | 9 (35) | 19 (100) | 40 (89) | / |
| Lentiginous growth above the sclerosis | 82 (91) | 19 (73) | 19 (100) | 44 (98) | / |
| Upward migration in the epidermis above the sclerosis | / | ||||
| No | 12 (13) | 9 (35) | 1 (5) | 2 (4) | |
| Lower half | 11 (12) | 7 (27) | 1 (5) | 3 (7) | |
| Focally in the upper half | 23 (26) | 9 (35) | 5 (26) | 9 (20) | |
| Prominent full thickness | 44 (48) | 1 (4) | 12 (63) | 31 (69) | |
| HMB-45 in the sclerotic component | 60 | 19 | 11 | 30 | / |
| Patchy, irregular | 37 (62) | 6 (32) | 10 (91) | 21 (70) | |
| Maturation or completely absent | 23 (38) | 13 (68) | 1 (9) | 9 (30) | |
| Ki67 in the sclerotic component | 66 | 19 | 13 | 34 | |
| No positivity in the lower half | 51 (77) | 19 (100) | 8 (62) | 24 (71) | / |
| Some positive melanocytes in the lower half, <5% overall | 13 (20) | 0 | 4 (31) | 9 (26) | |
| >5%, no gradient with the depth | 2 (3) | 0 | 1 (7) | 1 (3) | |
| Four-probe FISH in the sclerotic component | 41 | 16 | 7 | 18 | 0.001 |
| Positive | 14 (34) | 0 | 3 (43) | 11 (61) | |
| Negative | 27 (66) | 16 (100) | 4 (57) | 7 (39) |
Clinical and histopathologic parameters in relation to FISH results in 41 lesions with FISH analysis of the sclerotic component
| Parameter, n (%) | All | FISH-positive | FISH-negative | P -value |
|---|---|---|---|---|
| N (%) | 41 | 14 | 27 | |
| Age of the patients (years) (median; range) | 48; 22-89 | 51; 42-89 | 48; 22-77 | 0.196 |
| Thickness of sclerosis (mm) (mean; range) | 0.85; 0.5-1.5 | 1.0; 0.7-1.5 | 0.8; 0.5-1.3 | 0.002 |
| Location | 0.096 | |||
| Back | 25 (61) | 11 (79) | 14 (52) | |
| Other | 16 (39) | 3 (21) | 13 (48) | |
| Extension along eccrine ducts | 21/38 (55) | 11/14 (71) | 10/24 (42) | 0.026 |
| Evidence of regression | 8 (20) | 4 (29) | 4 (15) | 0.411 |
| Maturation in the sclerosis | 29 (71) | 7 (50) | 22 (81) | 0.068 |
| Dermal mitoses present | 10 (24) | 5 (36) | 5 (19) | 0.267 |
| Prominent nucleoli in the sclerosis | 20 (49) | 11 (79) | 9 (33) | 0.009 |
| Melanoma in situ adjacent to the sclerosis | 23 (56) | 13 (93) | 10 (39) | 0.001 |
| Severe cytological atypia in the epidermis above the sclerosis | 27 (66) | 12 (86) | 15 (56) | 0.053 |
| Prominent full thickness upward migration in the epidermis above the sclerosis | 19 (22) | 10 (71) | 9 (33) | 0.026 |
| Patchy irregular HMB-45 in the sclerotic component | 22/38 (58) | 11/13 (85) | 11/25 (44) | 0.016 |
| No Ki67 positivity in the lower half of the sclerotic component | 28/39 (72) | 7/14 (50) | 21/25 (84) | 0.033 |