The study of glycerol-based fermentation and broth downstream by nanofiltration

Marek Gryta; Agata Markowska-Szczupak; Joanna Grzechulska-Damszel; Justyna Bastrzyk; Marta Waszak

doi:10.2478/pjct-2014-0081

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The study of glycerol-based fermentation and broth downstream by nanofiltration

Green Sciences

Volume 16 (2014): Issue 4 (December 2014)

By: Marek Gryta, Agata Markowska-Szczupak, Joanna Grzechulska-Damszel, Justyna Bastrzyk and Marta Waszak

Open Access

|Dec 2014

Abstract

In this work, the glycerol fermentation was carried out using Citrobacter freundii bacteria. The influence of glycerol and metabolites concentrations, and the pH changes on the efficiency of 1,3-propanediol production, during batch and fed-batch processes, was presented. The nanofiltration was used for the separation of obtained post-fermentation solutions. The resulted 1,3-PD solutions were significantly desalted, which may facilitate further downstream processes during 1,3-PD production.

References

1. Saxena, R.K., Anand, P., Saran, S. & Isar, J. (2009). Microbial production of 1,3-propanediol: Recent developments and emerging opportunities. Biotechnol. Adv. 27, 895-913. DOI: 10.1016/j.biotechadv.2009.07.003.10.1016/j.biotechadv.2009.07.003
Search in Google Scholar
2. Nakamura, Ch.E. & Whited, G.M. (2009). Metabolic engineering for the microbial production of 1,3-propanediol. Curr. Opin. Biotechnol. 14, 454-459. DOI: 10.1016/j. copbio.2003.08.005.
Search in Google Scholar
3. Raynaud, C., Sarcabal, P., Meynial-Salles, I., Croux, Ch. & Soucaille, P. (1993). Molecular characterization of the 1,3-propanediol (1,3-PD) operon of Clostridium butyricum. Appl. Microbiol. Biotechnol. 38, 453-457. DOI: 10.1073_pnas.0734105100.
Search in Google Scholar
4. Metsoviti, M., Zeng, An.P., Koutinas, A.A. & Papanikolaou, S. (2013). Enhanced 1,3-propanediol production by a newly isolated Citrobacter freundii strain cultivated on biodiesel-derived waste glycerol through sterile and non-sterile bioprocesses. J. Biotechnol. 163, 408-418. DOI: 10.1016/j.jbiotec.2012.11.018.10.1016/j.jbiotec.2012.11.018
Search in Google Scholar
5. Anand, P. & Saxena, R.K. (2012). A comparative study of solvent-assisted pretreatment of biodiesel derived crude glycerol of growth and 1,3-propanediol production from Citrobacter freundii. New Biotechol. 29, 199-205. DOI: 10.1016/j.nbt.2011.05.010.10.1016/j.nbt.2011.05.010
Search in Google Scholar
6. Boenigk, R., Bowien, S. & Gottschalk, G. (1993). Fermentation of glycerol to 1,3-propanediol in continuous cultures of Citrobacter freundii. Appl. Microbiol. Biotechnol. 38, 453-457. DOI: 10.1007/BF00242936.10.1007/BF00242936
Search in Google Scholar
7. Colin, T., Bories, A., Lavigne, C. & Moulin, G. (2001). Effects of Acetate and Butyrate During Glycerol Fermentation by Clostridium butyricum. Curr. Microbiol. 43, 238-243. DOI: 10.1007/s002840010294.10.1007/s002840010294
Search in Google Scholar
8. Ferreira, T.F., Ribeiro, R.R., Ribeiro, C.M.S., Freire, D.M.G. & Coelho, M.A.Z. (2012). Evaluation of 1,3-Propanediol Production from Crude Glycerol by Citrobacter freundii ATCC 8090. Chem. Engin. Transactions. 27, 157-162. DOI: 10.3303/CET1438080.
Search in Google Scholar
9. Barbirato, F., Grivet, J.P., Soucaille, P. & Bories, A. (1996). 3-Hydroxypropionaldehyde, an Inhibitory Metabolite of Glycerol Fermentation to 1,3-Propanediol by Enterobacterial Species. Appl. Environ. Microbiol. 62(4), 1448-1451.10.1128/aem.62.4.1448-1451.1996
Search in Google Scholar
10. Tomczak, W. & Gryta, M. (2013). The application of ultrafiltration for separation of glycerol solution fermented by bacteria. Pol. J. Chem. Tech. 15(3), 115-120. DOI: 10.2478/pjct-2013-0057.10.2478/pjct-2013-0057
Search in Google Scholar
11. Barbirato, F., Himmi, El H., Conte, T. & Bories, A. (1998). 1,3-propanediol production by fermentation: An interesting way to valorize glycerin from the ester and ethanol industries. Ind. Crops Prod. 7, 281-289. DOI: 10.1016/S0926-6690(97)00059-9.10.1016/S0926-6690(97)00059-9
Search in Google Scholar
12. Yanga, Ch., Jianga, P., Xiaoa, S., Zhanga, Ch., Loub, K. & Xinga, X.H. (2011). Fed-batch fermentation of recombinant Citrobacter freundii with expression of a violacein-synthesizing gene cluster for efficient violacein production from glycerol. Biochem. Eng. J. 57, 55-62. DOI:10.1016/j.bej.2011.08.008.10.1016/j.bej.2011.08.008
Search in Google Scholar
13. Russel, J.B. (1992). Another explanation for the toxicity of fermentation acids at low pH: anion accumulation versus uncoupling. J. Appl. Bacteriol. 73, 363-370. DOI: 10.1111/j.1365-2672.1992.tb04990.x.10.1111/j.1365-2672.1992.tb04990.x
Search in Google Scholar
14. Biebl, H. (1991). Glycerol fermentation of 1,3-propanediol by Clostridium butyricum. Measurement of product inhibition by use a pH-auxostat. Appl Microbiol. Biotechnol. 35, 701-705. DOI: 10.1007/BF00169880.10.1007/BF00169880
Search in Google Scholar
15. Colin, T., Bories, A. & Moulin, G. (2000). Inhibition of Clostridium butyricum by 1,3-propanediol and diols during glycerol fermentation. Appl. Microbiol. Biotechnol. 54, 201-205. DOI: 10.1007/s002530000365.10.1007/s00253000036510968633
Search in Google Scholar
16. Zeng, A.P., Ross, A., Biebl, H., Tag, C., Günzel, B. & Deckwer, W.D. (1994). Multiple product inhibition and growth modeling of Clostridium butyricum and Klebsiellia pneumoniae in glycerol fermentation. Biotechnol. Bioeng. 44, 902-911. DOI: 10.1002/bit.260440806.10.1002/bit.26044080618618908
Search in Google Scholar
17. Homann, T., Tag, C., Biebl, H., Deckwer, W.D. & Schink, B. (1990). Fermentation of glycerol by Klebsiella and Citrobacter strains. Appl. Microbiol. Biotechnol. 33, 121-126. DOI: 10.1007/BF00176511.10.1007/BF00176511
Search in Google Scholar
18. Annand, P., Saxena, R.K. & Marwah, R.G. (2011). A novel downstream process for 1,3-propanediol from glycerol-based fermentation. Appl. Microbiol. Biotechnol. 90, 1267-1276. DOI: 10.1007/s00253-011-3161-2.10.1007/s00253-011-3161-221360149
Search in Google Scholar
19. Anand, P. & Saxena, R.K. (2012). A comparative study of solvent-assisted pretreatment of biodiesel derived crude glycerol of growth and 1,3-propanediol production from Citrobacter freundii. New Biotechol. 29, 199-205. DOI: 10.1016/j.nbt.2011.05.010.10.1016/j.nbt.2011.05.01021689798
Search in Google Scholar
20. Wu, R.Ch., Ren, H.J., Xu, Y. & Liu, D. (2010). The final recover of salt from 1,3-propanadiol fermentation broth. Sep. Purif. Technol. 73, 122-125. DOI: 10.1016/j. seppur.2010.03.013.
Search in Google Scholar
21. Bastrzyk, J., Gryta, M. & Karakulski, K. (2014). Fouling of nanofiltration membranes used for separation of fermented glycerol solutions. Chem. Pap. 68 (6), 757-765. DOI: 10.2478/s11696-013-0520-8.10.2478/s11696-013-0520-8
Search in Google Scholar
22. Himstedt, H.H., Marshall, K.M. & Wickramasinghe, S.R. (2011). pH-responsive nanofiltration membranes by surface modification. J. Membr. Sci. 366, 373-381. DOI: 10.1016/j.memsci.2010.10.027.10.1016/j.memsci.2010.10.027
Search in Google Scholar
23. Luo, J., & Wan, Y. (2011). Effect of highly concentrated salt on retention of organic solutes by nanofiltration polymeric membranes. J. Membr. Sci. 372, 145-153. DOI: 10.1016/j.memsci.2011.01.066.10.1016/j.memsci.2011.01.066
Search in Google Scholar
24. Mohammad, A.W., Basha, R.K. & Leo, C.P. (2010). Nanofiltration of glucose solution containing salts: Effects of membrane characteristics, organics component and salts on retention. J. Food Eng. 97, 510-518. DOI: 10.1016/j.jfoodeng.2009.11.010.10.1016/j.jfoodeng.2009.11.010
Search in Google Scholar

Articles in this issue

DOI: https://doi.org/10.2478/pjct-2014-0081 | Journal eISSN: 3072-0389

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Language: English

Page range: 117 - 122

Published on: Dec 11, 2014

Published by: West Pomeranian University of Technology, Szczecin

In partnership with: Paradigm Publishing Services

Publication frequency: Volume open

Keywords:

glycerol fermentation,

1,3-propanediol,

nanofiltration,

Citrobacter freundii

Related subjects:

Industrial chemistry,

Biotechnology,

Chemical engineering,

Process engineering

© 2014 Marek Gryta, Agata Markowska-Szczupak, Joanna Grzechulska-Damszel, Justyna Bastrzyk, Marta Waszak, published by West Pomeranian University of Technology, Szczecin
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Volume 16 (2014): Issue 4 (December 2014)