The application of ultrafiltration for separation of glycerol solution fermented by bacteria

Tomczak, Wirginia; Gryta, Marek

doi:10.2478/pjct-2013-0057

Abstract

A biotechnological synthesis generated a fermentation broth containing the dissociated forms of organic compounds and residual mineral salts. An effective method of selective removal of the ionic species and organic compounds from solutions comprises nanofiltration and reverse osmosis. Ultrafiltration (UF) was used in this work as a pre-treatment method for the preparation of feed for these processes. The UF study was carried out with a real broth, which was obtained using Citrobacter freundii bacteria for the fermentation of glycerol solutions, resulting in the UF permeate with turbidity below 0.1 NTU. However, a significant decline of the permeate flux was observed during the UF process. The influence of the transmembrane pressure on the fouling intensity of used ceramic membranes was investigated. A periodical membrane cleaning was carried out by rinsing with water and a 1 wt % solution of sodium hydroxide. The applied cleaning procedures permitted to restore the initial permeate flux.

References

1. Gungormusler, M., Gonen, C. & Azbar, N. (2011). Continuous production of 1,3-propanediol using raw glycerol with immobilized Clostridium beijerinckii NRRL B-593 in comparison to suspended culture, Bioprocess Biosyst. Eng. 34, 727-733. DOI: 10.1007/s00449-011-0522-2.10.1007/s00449-011-0522-2
Search in Google Scholar
2. Liu, B., Christiansen, K., Parnas, R., Xu, Z. & Li, B. (2013). Optimizing the production of hydrogen and 1,3-propanediol in anaerobic fermentation of biodiesel glycerol, Int. J. HydrogenEnergy, 383, 196-205. DOI: 10.1016/j.ijhydene.2012.12.135.10.1016/j.ijhydene.2012.12.135
Search in Google Scholar
3. Leja, K., Czaczyk, K. & Myszka, K. (2011). The use of microorganisms in 1,3-Propanediol production, Afr. J. Microbiol. Res., 5 (26), 4652-4658. DOI: 10.5897/AJMR11.847.10.5897/AJMR11.847
Search in Google Scholar
4. Raynaud, C., Sarcabal, P., Meynial-Salles, I., Croux, Ch. & Soucaille, P. (1993). Molecular characterization of the 1,3-propanediol (1,3-PD) operon of Clostridium butyricum, Appl. Microbiol. Biotechnol., 38, 453-457. DOI: 10.1073_pnas.0734105100.
Search in Google Scholar
5. Barbirato, F., Himmi, El H., Conte, T. & Bories, A. (1998). 1,3-propanediol production by fermentation: An interesting way to valorize glycerin from the ester and ethanol industries, Ind. Crops Prod., 7, 281-289. DOI: 10.1016/S0926-6690(97)00059-9.10.1016/S0926-6690(97)00059-9
Search in Google Scholar
6. Metsoviti, M., Zeng, An.P., Koutinas, A.A. & Papanikolaou, S. (2013). Enhanced 1,3-propanediol production by a newly isolated Citrobacter freundii strain cultivated on biodiesel-derived waste glycerol through sterile and non-sterile bioprocesses, J. Biotechnol., 163, 408-418. DOI: 10.1016/j.jbiotec.2012.11.018.10.1016/j.jbiotec.2012.11.01823220217
Search in Google Scholar
7. Anand, P. & Saxena, R.K. (2012). A comparative study of solvent-assisted pretreatment of biodiesel derived crude glycerol of growth and 1,3-propanediol production from Citrobacter freundii, New Biotechol., 29, 199-205. DOI: 10.1016/j. nbt.2011.05.010.
Search in Google Scholar
8. Boenigk, R., Bowien, S. & Gottschalk, G. (1993). Fermentation of glycerol to 1,3-propanediol in continuous cultures of Citrobacter freundii, Appl. Microbiol. Biotechnol., 38, 453-457. DOI: 10.1007/BF00242936.10.1007/BF00242936
Search in Google Scholar
9. Xiu, Z.L. & Zeng, A.P. (2008). Present state and perspective of downstream processing of biologically produced 1,3-propanediol and 2,3-butanediol. Appl. Microbiol. Biotechnol., 78, 917-926. DOI: 10.1007/s00253-008-1387-4.10.1007/s00253-008-1387-418320188
Search in Google Scholar
10. Li, Z., Jiang, B., Hang, D. & Xiu, Z. ( 2009). Aqueous two-phase extraction of 1,3-propanediol from glycerol-based fermentation broths, Sep. Purif. Technol., 66, 472-478. DOI: 10.1016/j.seppur.2009.02.009.10.1016/j.seppur.2009.02.009
Search in Google Scholar
11. Wu, R.C., Ren, H.J. Xu, Y.Z. & Liu, D.H. (2010). The final recover of salt from 1,3-propanadiol fermentation broth. Sep. Purif. Technol, 73, 122-125. DOI: 10.1016/j.seppur.2010.03.013.10.1016/j.seppur.2010.03.013
Search in Google Scholar
12. Annand, P., Saxena, R.K. & Marwah, R.G. (2011). A novel downstream process for 1,3-propanediol from glycerol- -based fermentation, Appl. Microbiol. Biotechnol., 90, 1267-1276. DOI: 10.1007/s00253-011-3161-2.10.1007/s00253-011-3161-221360149
Search in Google Scholar
13. Vellenga, E. & Trägårdh, G. (1998). Nanofiltration of combined salt and sugar solutions: coupling between retentions. Desalination, 120, 211-220. DOI: 10.1016/s0011-9164(98)00219-7.10.1016/S0011-9164(98)00219-7
Search in Google Scholar
14. Schäfer, A.I., Fane, A.G. & Waite, T.D. (Eds.). (2005). Nanofiltration: Principles and applications. Oxford, UK: Elsevier Advanced Technology.
Search in Google Scholar
15. Bonnélye, V., Guey, L. & Del Castillo, J. (2008). UF/ MF as RO pre-treatment: the real benefit, Desalination, 222, 59-65. DOI: 10.1016/j.desal.2007.01.129.10.1016/j.desal.2007.01.129
Search in Google Scholar
16. Blanpain-Avet, P., Migdal, J.F. & Bénézech, T. (2009). Chemical cleaning of a tubular ceramic microfiltration membrane fouled with a whey protein concentrate suspension-Characterization of hydraulic and chemical cleanliness, J. Membr. Sci., 337, 153-174. DOI: 10.1016/j.memsci.2009.03.033.10.1016/j.memsci.2009.03.033
Search in Google Scholar
17. Blanpain-Avet, P., Migdal, J.F. & Bénézech, T. (2004). The effect of multiple fouling and cleaning cycles on a tubular ceramic microfiltration membrane fouled with z whey protein concentrate, Food Bioproducts Process., 82 (C3), 231-234. DOI: 10.1205/fbio.82.3.231.44182.10.1205/fbio.82.3.231.44182
Search in Google Scholar
18. Ogunbiyi, O.O., Miles, N.J. & Hilal, N. (2008). The effects of performance and cleaning cycles of new tubular ceramic microfiltration membrane fouled with a model yeast suspension, Desalination, 220, 273-289. DOI: 10.1016/j.desal.2007.01.034.10.1016/j.desal.2007.01.034
Search in Google Scholar
19. Juang, R.S., Chen, H.L. & Chen, Y.S. (2008). Resistance-in-series analysis in cross-flow ultrafiltration of fermentation broths of Bacillus subtilis culture, J. Membr. Sci., 323, 193-200. DOI: 10.1016/j.memsci.2008.06.032.10.1016/j.memsci.2008.06.032
Search in Google Scholar
20. Markardij, A., Chen, X.D. & Farid, M.M. (1999). Microfiltration and ultrafiltration of milk: some aspects of fouling and cleaning, Food Bioproducts Process., 77, 107-113. DOI: 10.1205/096030899532394.10.1205/096030899532394
Search in Google Scholar
21. Karasu, K., Glennon, N., Lawrence, N.D., Stevens, G.W., O’Connor, J.O., Barber, A.R., Yoshikawa, S. & Kentish, S.E. (2010). A comparison between ceramic and polymeric membrane systems for casein concentrate manufacture, Int. J. Dairy Technol., 63 (2), 284-289. DOI: 10.1111/j.1471-0307.2010.00582.x.10.1111/j.1471-0307.2010.00582.x
Search in Google Scholar
22. Hwang, K.J., Wang, T.T., Iritani, E. & Katagiri, N. (2010). Effect of gel particle softness on the performance of cross-flow microfiltration, J. Membr. Sci., 35, 130-137. DOI: 10.1016/j. memsci.2010.08.043.
Search in Google Scholar
23. Kazemimoghadam, M. & Mohammadi, T. (2007). Chemical cleaning of ultrafiltration membranes in the milk industry, Desalination, 204, 213-218. DOI: 10.1016/j.desal.2006.04.030.10.1016/j.desal.2006.04.030
Search in Google Scholar
24. Blanpain-Avet, P., Migdal, J.F. & Bénézech, T. (2004). The effect of multiple fouling and cleaning cycles on a tubular ceramic microfiltration membrane fouled with a whey protein concentrate. Membrane performance and cleaning efficiency, Food Bioproducts Process, 82 (C3), 231-243. DOI: 10.1205/ fbio.82.3.231.44182.10.1205/fbio.82.3.231.44182
Search in Google Scholar
25. Cabero, M.L., Riera, F.A. & Alvarez, R. (1999). Rinsing of ultrafiltration ceramic membranes fouled with whey proteins: effects on cleaning procedures, J. Membr. Sci. 154, 239-250. DOI: 10.1016/S0376-7388(98)00294-4.10.1016/S0376-7388(98)00294-4
Search in Google Scholar
26. Bachin, P., Aimar, P. & Field, R.W. (2006). Critical and sustainable fluxes: Theory, experiments and applications, J. Membr. Sci., 281, 42-69. DOI: 10.1016/j.memsci.2006.04.014.10.1016/j.memsci.2006.04.014
Search in Google Scholar
27. Nigam, M.O., Bansal, B. & Chen, X.D. (2008). Fouling and cleaning of whey protein concentrate fouled ultrafiltration membranes, Desalination, 218, 313-322. DOI: 10.1016/j. desal.2007.02.027.
Search in Google Scholar
28. Madaeni, S.S. & Mansourpanah, Y. (2004). Chemical cleaning of reverse osmosis membranes fouled by whey, Desalination, 161, 13-24. DOI: 10.1016/S0011-9164(04)90036-7. 10.1016/S0011-9164(04)90036-7
Search in Google Scholar

The application of ultrafiltration for separation of glycerol solution fermented by bacteria

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