In vitro effects of β-cyclodextrin-complexed rufinamide on magnesium-free artificial cerebrospinal fluid-induced epileptiform neural activity
References
- Brandon M K. Common Substrates of Medication-Resistant Temporal Lobe Epilepsy: Hippocampal Sclerosis, Focal Cortical Dysplasia, and Combined Pathologies. Transl Neurosci Res Rev 2019;2. https://doi.org/10.36959/817/522.
- Thom M, Eriksson S, Martinian L, et al. Temporal Lobe Sclerosis Associated With Hippocampal Sclerosis in Temporal Lobe Epilepsy: Neuropathological Features. J Neuropathol Exp Neurol 2009;68:928–38. https://doi.org/10.1097/NEN.0b013e3181b05d67.
- Perucca E, Cloyd J, Critchley D, et al. Rufinamide: Clinical pharmacokinetics and concentration–response relationships in patients with epilepsy. Epilepsia 2008;49:1123–41. https://doi.org/10.1111/j.1528-1167.2008.01665.x.
- Kim JY, Lee CG, Yu HJ, et al. The Efficacy and Tolerability of Rufinamide in Intractable Pediatric Epilepsy. J Epilepsy Res 2012;2:33–7. https://doi.org/10.14581/jer.12009.
- Arroyo S. Rufinamide. Neurotherapeutics 2007;4:155–62. https://doi.org/10.1016/j.nurt.2006.11.006.
- Huang L, Xiao W, Wang Y, et al. Metabotropic glutamate receptors (mGluRs) in epileptogenesis: an update on abnormal mGluRs signaling and its therapeutic implications. Neural Regen Res 2023;19:360–8. https://doi.org/10.4103/1673-5374.379018.
- Mazzucchelli I, Rapetti M, Fattore C, et al. Development and validation of an HPLC-UV detection assay for the determination of rufinamide in human plasma and saliva. Analytical and Bioanalytical Chemistry 2011;401:1013–21. https://doi.org/10.1007/s00216-011-5126-9.
- Vecsernyés M, Fenyvesi F, Bácskay I, et al. Cyclodextrins, Blood–Brain Barrier, and Treatment of Neurological Diseases. Archives of Medical Research 2014;45:711–29. https://doi.org/10.1016/j.arcmed.2014.11.020.
- Szabó Z-I, Gál R, Gall Z, et al. Cyclodextrin complexation improves aqueous solubility of the antiepileptic drug, rufinamide: solution and solid state characterization of compound-cyclodextrin binary systems. Journal of Inclusion Phenomena and Macrocyclic Chemistry 2017;88:1–10. https://doi.org/10.1007/s10847-017-0710-z.
- Pytel M, Mercik K, Mozrzymas JW. Interaction between cyclodextrin and neuronal membrane results in modulation of GABAA receptor conformational transitions. British J Pharmacology 2006;148:413–22. https://doi.org/10.1038/sj.bjp.0706747.
- Anderson WW, Lewis DV, Swartzwelder HS. BRE 21901 Magnesium-free medium activates seizure-like events in the rat hippocampal slice n.d.
- Mody I, Lambert JD, Heinemann U. Low extracellular magnesium induces epileptiform activity and spreading depression in rat hippocampal slices. Journal of Neurophysiology 1987;57:869–88. https://doi.org/10.1152/jn.1987.57.3.869.
- Dulla CG, Janigro D, Jiruska P, et al. How do we use in vitro models to understand epileptiform and ictal activity? A report of the TASK1‐WG4 group of the ILAE/AES Joint Translational Task Force. Epilepsia Open 2018;3:460–73. https://doi.org/10.1002/epi4.12277.
- Zhang ZJ, Koifman J, Shin DS, et al. Transition to Seizure: Ictal Discharge Is Preceded by Exhausted Presynaptic GABA Release in the Hippocampal CA3 Region. J Neurosci 2012;32:2499–512. https://doi.org/10.1523/JNEUROSCI.4247-11.2012.
- Carlson HL, Laliberté C, Brooks BL, et al. Reliability and variability of diffusion tensor imaging (DTI) tractography in pediatric epilepsy. Epilepsy & Behavior 2014;37:116–22. https://doi.org/10.1016/j.yebeh.2014.06.020.
- Leys C, Ley C, Klein O, et al. Detecting outliers: Do not use standard deviation around the mean, use absolute deviation around the median. Journal of Experimental Social Psychology 2013;49:764–6. https://doi.org/10.1016/j.jesp.2013.03.013.
- Kiss RJ, Nagy ZA, Szentes Á, et al. In vitro modulation of seizure-like activity with β-Cyclodextrin-complexed Rufinamide. Acta Marisiensis - Seria Medica 2024;70. https://doi.org/10.2478/amma-2024-0020.
- Huusko N, Römer C, Ndode-Ekane XE, et al. Loss of hippo-campal interneurons and epileptogenesis: a comparison of two animal models of acquired epilepsy. Brain Struct Funct 2015;220:153–91. https://doi.org/10.1007/s00429-013-0644-1.
- Cameron MC, Zhan R, Nadler JV. Morphologic integration of hilar ectopic granule cells into dentate gyrus circuitry in the pilocarpine model of temporal lobe epilepsy. J of Comparative Neurology 2011;519:2175–92. https://doi.org/10.1002/cne.22623.
- Gáll Z, Vancea S, Szilágyi T, et al. Dose-dependent pharmacokinetics and brain penetration of rufinamide following intravenous and oral administration to rats. European Journal of Pharmaceutical Sciences 2015;68:106–13. https://doi.org/10.1016/j.ejps.2014.12.012.
DOI: https://doi.org/10.2478/orvtudert-2025-0006 | Journal eISSN: 2537-5059 | Journal ISSN: 1453-0953
Language: English
Page range: 74 - 85
Published on: Jul 2, 2026
Published by: Transylvanian Museum Society
In partnership with: Paradigm Publishing Services
Publication frequency: 2 issues per year
Keywords:
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© 2026 Előd Bomher, Zsolt-András Nagy, Ádám Szentes, Rita-Judit Kiss, Károly Orbán-Kis, Tibor Szilágyi, published by Transylvanian Museum Society
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.