
Figure 1
P. nigra recruitment pattern (number of individuals, mean ± SE, n=4) on artificial substrates submerged in vertical and horizontal positions

Figure 2
Abundance of P. nigra on different treatment panels used in this study. Abundance (mean ± SE 225 cm−2) data were pooled for each factor and all treatments. a) Recruitment pattern on different substrate types. b) Abundance of the ascidian on substrates indifferent colors. c) Abundance of the ascidian on panels submerged in vertical and horizontal orientation. d) Recruitment pattern on panels submerged in the shade and exposed to light

Figure 3
Seasonal recruitment pattern of P. nigra on artificial materials submerged in the central Red Sea. a) Pooled data for the seasonal abundance of the ascidian on different types of substrate b) Pooled data for the seasonal abundance of the ascidian on substrates in different colors. Abundance (mean ± SE) for the panel surface area of 225 cm2

Figure 4
Interactive plot of P. nigra recruitment on different substrates submerged in vertical and horizontal orientations
Table 1
Three-way ANOVA results of P. nigra recruitment on different types of substrate (p<0.05 = significant effect). Season, substrate type and orientation were used as factors for ANOVA
| SS | Df | MS | F | p | |
|---|---|---|---|---|---|
| Season | 5.9063 | 3 | 1.9687 | 1.0989 | 0.353 |
| Substrate | 8.9063 | 3 | 2.9687 | 1.6570 | 0.181 |
| Orientation | 0.1250 | 1 | 0.1250 | 0.0697 | 0.792 |
| Season*Substrate | 43.1562 | 9 | 4.7951 | 2.6765 | 0.008 |
| Season*Orientation | 3.9375 | 3 | 1.3125 | 0.7326 | 0.535 |
| Substrate*Orientation | 17.9375 | 3 | 5.9791 | 3.3374 | 0.022 |
| Season*Substrate*Orientation | 18.2500 | 9 | 2.0277 | 1.1318 | 0.348 |
| Error | 173.7813 | 97 | 1.7915 |
Table 2
Three-way ANOVA results for the ascidian P. nigra recruitment on substrates in different colors submerged in the shade and exposed to light. Season, substrate color and light were used as factors (p<0.05 = significant effect)
| SS | Df | MS | F | p | |
|---|---|---|---|---|---|
| Season | 42.0500 | 1 | 42.0500 | 3.5207 | 0.065 |
| Color | 135.3250 | 4 | 33.8312 | 2.8326 | 0.031 |
| Light | 88.2000 | 1 | 88.2000 | 7.3848 | 0.008 |
| Season*Color | 105.0750 | 4 | 26.2687 | 2.1994 | 0.079 |
| Season*Light | 9.8000 | 1 | 9.80000 | 0.8205 | 0.368 |
| Color*Light | 97.9250 | 4 | 24.4812 | 2.0497 | 0.098 |
| Season*Color*Light | 137.0750 | 4 | 34.2687 | 2.8692 | 0.030 |
| Error | 728.5500 | 61 | 11.9434 |

Figure 5
Interactive plot of P. nigra recruitment on substrates in different colors submerged in the shade and exposed to light

Figure 6
Abundance (number of individuals, mean ± SE, n=4) of P. nigra on panels in different colors submerged in the shade and exposed to light