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Deuterium isotope effects in mechanistic studies of biotransformations of l-tyrosine and p-hydroxyphenylpyruvic acid catalyzed by the enzyme l-phenylalanine dehydrogenase Cover

Deuterium isotope effects in mechanistic studies of biotransformations of l-tyrosine and p-hydroxyphenylpyruvic acid catalyzed by the enzyme l-phenylalanine dehydrogenase

Nukleonika
Volume 70 (2025): Issue 2 (June 2025)
By: ,  ,   and    
Open Access
|May 2025
Figures & tables

Figures & Tables

Fig. 1.

Enforced metabolic pathways of accumulated l-Phe and l-Tyr in a person suffering from PKU and type I tyrosinemia diseases.

Fig. 2.

Alternative metabolic pathways of l-Tyr in a person suffering from type II tyrosinemia disease.

Fig. 3.

Reversible conversion of l-Phe and l-Tyr into corresponding oxoacids catalyzed by the enzyme PheDH.

Table 1.

Solvent isotope effects (SIE) and kinetic isotope effects (KIE) in the oxidative deamination of l-Tyr and reductive amination of PPA catalyzed by enzyme PheDH

ReactantSIE
on Vmaxon Vmax/KM
l-Tyr1.43 ± 0.091.48 ± 0.12
PPA1.44 ± 0.081.58 ± 0.14
on Vmaxon Vmax/KM
l-Tyr/[2-2H]-l-Tyr2.26 ± 0.102.87 ± 0.11
PPA/[(3S)-2H]-PPA1.55 ± 0.061.53 ± 0.16
DOI: https://doi.org/10.2478/nuka-2025-0006 | Journal eISSN: 1508-5791 (formerly 0029-5922) | Journal ISSN: 0029-5922
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Language: English
Page range: 51 - 56
Submitted on: Jan 5, 2024
Accepted on: Mar 4, 2025
Published on: May 2, 2025
Published by: Institute of Nuclear Chemistry and Technology
In partnership with: Paradigm Publishing Services
Keywords:
Kinetic isotope effects,
l-Phenylalanine,
Phenylalanine dehydrogenase,
p-Hydroxyphenylpyruvic acid,
Solvent isotope effects,
Tyrosine
Related subjects:
Chemistry,
Nuclear chemistry

© 2025 Katarzyna Pałka, Katarzyna Podsadni, Jolanta Szymańska-Majchrzak, Elżbieta Winnicka, published by Institute of Nuclear Chemistry and Technology
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Volume 70 (2025): Issue 2 (June 2025)
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