Fig. 1A and B
Biochemical profile assessment of Pasteurella multocida strains using the API 20E
| Reaction/enzyme | Number of strains showing positive reactions (%) | ||
|---|---|---|---|
| P. multocida subsp. multocida | P. multocida subsp. multocida ornithine-negative | P. multocida subsp. septica | |
| β-galactosidase | 0 | 0 | 0 |
| Arginine dihydrolase | 0 | 0 | 0 |
| Lysine decarboxylase | 0 | 0 | 0 |
| Ornithine decarboxylase | 20(100) | 0 | 3 (100) |
| Citrate utilisation | 0 | 0 | 0 |
| Hydrogen sulphide production | 0 | 0 | 0 |
| Urease | 0 | 0 | 0 |
| Tryptophan deaminase | 0 | 0 | 0 |
| Indole production | 17(85) | 92 (100) | 1 (33) |
| Acetoin production | 0 | 0 | 0 |
| Gelatinase | 0 | 0 | 0 |
| D-glucose | 20 (100) | 92 (100) | 3 (100) |
| D-mannitol | 20 (100) | 92 (100) | 3 (100) |
| Inositol | 0 | 0 | 0 |
| D-sorbitol | 20 (100) | 92 (100) | 0 |
| L-rhamnose | 0 | 0 | 0 |
| D-saccharose | 20 (100) | 92 (100) | 3 (100) |
| D-melibiose | 0 | 0 | 0 |
| Amygdalin | 0 | 0 | 0 |
| L-arabinose | 0 | 0 | 0 |
| Cytochrome oxidase | 20 (100) | 92 (100) | 3 (100) |
| Nitrate reduction | 20 (100) | 92 (100) | 3 (100) |
| Total | 20 | 92 | 3 |
Distribution of Pasteurella multocida capsular antigens
| Somatic antigen number of strains (%) | Number of strains (%) | |||
|---|---|---|---|---|
| 3 | 12 | |||
| Capsular antigen | A | 38 (37.6) | 63 (62.4) | 101 (87.8) |
| D | 2 (20.0) | 8 (80.0) | 10 (8.7) | |
| F | 1 (25.0) | 3 (75.0) | 4 (3.5) | |
| Total | 41 | 74 | 115 | |
Distribution of Pasteurella multocida somatic antigens
| Capsular antigen numer of strains (%) | Number of strains (%) | ||||
|---|---|---|---|---|---|
| A | D | F | |||
| Somatic antigen | 3 | 38 (92.7) | 2 (4.9) | 1 (2.4) | 41 (35.7) |
| 12 | 63 (85.1) | 8 (10.8) | 3 (4.1) | 74 (64.3) | |
| Total | 101 | 10 | 4 | 115 | |
Sugar fermentation of Pasteurella multocida strains isolated from rabbits
| Sugar | Number of strains showing positive reactions (%) | ||
|---|---|---|---|
| P. multocida subsp. multocida | P. multocida subsp. multocida ornithinenegative | P. multocida subsp. septica | |
| Dulcitol | 0 | 0 | 0 |
| Trehalose | 7 (35) | 0 | 0 |
| Xylose | 17(85) | 92 (100) | 3 (100) |
| Maltose | 0 | 0 | 0 |
| Total | 20 | 92 | 3 |
Biochemical profile assessment of Pasteurella multocida strains using the ID 32E
| Reaction/enzyme | Number of strains showing positive reactions (%) | ||
|---|---|---|---|
| P. multocida subsp. multocida | P. multocida subsp. multocida ornithine-negative | P. multocida subsp. septica | |
| Ornithine decarboxylase | 20(100) | 0 | 3 (100) |
| Arginine dihydrolase | 0 | 0 | 0 |
| Lysine decarboxylase | 0 | 0 | 0 |
| Urease | 0 | 0 | 0 |
| L-arabitol | 0 | 0 | 0 |
| Galacturonate | 0 | 0 | 0 |
| 5-ketogluconate | 0 | 0 | 0 |
| Lipase | 0 | 0 | 0 |
| Phenol red | 0 | 0 | 0 |
| β-glucosidase | 0 | 0 | 0 |
| Mannitol | 20 (100) | 92(100) | 3 (100) |
| Maltose | 0 | 0 | 0 |
| Adonitol | 0 | 0 | 0 |
| Palatinose | 0 | 0 | 0 |
| β-glucuronidase | 0 | 0 | 0 |
| Malonate | 0 | 0 | 0 |
| Indole production | 17 (85) | 92 (100) | 1 (33) |
| N-acetyl-β-glucosaminidase | 0 | 0 | 0 |
| β-galactosidase | 0 | 0 | 0 |
| Glucose | 20 (100) | 92 (100) | 3 (100) |
| Sucrose | 20 (100) | 92 (100) | 3 (100) |
| L-arabinose | 0 | 0 | 0 |
| D-arabitol | 0 | 0 | 0 |
| α-glucosidase | 0 | 0 | 0 |
| α-galactosidase | 0 | 0 | 0 |
| Trehalose | 7 (35) | 0 | 0 |
| Rhamnose | 0 | 0 | 0 |
| Inositol | 0 | 0 | 0 |
| Cellobiose | 0 | 0 | 0 |
| Sorbitol | 20 (100) | 92 (100) | 0 |
| α-maltosidase | 0 | 0 | 0 |
| L-aspartic acid arylamidase | 0 | 0 | 0 |
| Total | 20 | 92 | 3 |