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First molecular identification and phylogenetic analysis of Neospora caninum in dogs from Sulaymaniyah province, Iraq Cover

First molecular identification and phylogenetic analysis of Neospora caninum in dogs from Sulaymaniyah province, Iraq

Journal of Veterinary Research
AHEAD OF PRINT
By: Aram Ahmad Mohammed and  Taib Ahmed Hama Soor  
Open Access
|Jan 2026

Figures & Tables

Fig. 1.

Multiple sequence alignment of Neospora caninum isolates from dogs in Sulaymaniyah, Iraq, alongside other GenBank isolates. The alignment was conducted using the ClustalW algorithm on partial Nc5 gene sequences. The nucleotide sequences from this study are assigned accession Nos: PQ850578–PQ850587
Multiple sequence alignment of Neospora caninum isolates from dogs in Sulaymaniyah, Iraq, alongside other GenBank isolates. The alignment was conducted using the ClustalW algorithm on partial Nc5 gene sequences. The nucleotide sequences from this study are assigned accession Nos: PQ850578–PQ850587

Fig. 2.

Phylogenetic tree of Neospora caninum isolates from dogs in Sulaymaniyah, Iraq, constructed using the maximum-likelihood method based on Nc5 gene sequences. The accession Nos: PQ850578–PQ850587 represent the sequences analysed in this study. The scale bar indicates a 1% nucleotide difference
Phylogenetic tree of Neospora caninum isolates from dogs in Sulaymaniyah, Iraq, constructed using the maximum-likelihood method based on Nc5 gene sequences. The accession Nos: PQ850578–PQ850587 represent the sequences analysed in this study. The scale bar indicates a 1% nucleotide difference

Global Neospora caninum GenBank sequences and accession numbers used for phylogenetic analysis and multiple sequence alignment

Protozoan speciesGenBank accession No.Geographical originHost organismCitation
N. caninumOP244818IraqSheepEssa (17)
MT955657 CattleGharekhani et al. (19)
MT955656Iran
MT709298
MT709296 Dog
MT346029ItalyRed foxZanet et al. (36)
KP715569ItalyDeerZanet et al., 2015, unpublished
LN714476UK-Ramaprasad et al. (29)
KF649845USAWolfDubey et al. (10)

Association of risk factors with N_ caninum infection in dogs from Sulaymaniyah Province, Iraq

Number of dogs examinedNumber (%) of dogs infectedX2 (P-value)
Overall25810 (3.87)
LifestyleStray dogs1419 (6.38)0.022 (<0.05)
Domestic dogs1171 (0.85)
SexMale1248 (6.45)0.039 (<0.05)
Female1342 (1.49)
AgeYoung (≤12 months)1312 (1.52)0.047 (<0.05)
Adult (>12 months)1278 (6.30)
DOI: https://doi.org/10.2478/jvetres-2026-0001 | Journal eISSN: 2450-8608
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Language: English
Submitted on: May 7, 2025
|
Accepted on: Jan 8, 2026
|
Published on: Jan 13, 2026
Published by: National Veterinary Research Institute in Pulawy
In partnership with: Paradigm Publishing Services
Publication frequency: 4 issues per year
Keywords:
abortigenic disease,
canine parasite,
Nc5 gene,
partial Sanger sequencing,
phylogeny
Related subjects:
Life sciences,
Molecular biology,
Microbiology and virology,
Life sciences, other,
Medicine,
Veterinary medicine

© 2026 Aram Ahmad Mohammed, Taib Ahmed Hama Soor, published by National Veterinary Research Institute in Pulawy
This work is licensed under the Creative Commons Attribution 4.0 License.

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