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Repression of the fliC gene as an immune evasion strategy in Yersinia ruckeri infection of rainbow trout (Oncorhynchus mykiss) Cover

Repression of the fliC gene as an immune evasion strategy in Yersinia ruckeri infection of rainbow trout (Oncorhynchus mykiss)

Open Access
|Oct 2025

Figures & Tables

Fig. 1.

Frequency of clinical symptoms and pathological changes in rainbow trout challenged with fliC gene–repressed (024) and fliC gene–expressing (R57) Yersinia ruckeri strains. The result is the average of two repetitions. L – low infectious dose; H – high infectious dose. Colour gradient corresponds to the mean number of individuals showing clinical symptoms
Frequency of clinical symptoms and pathological changes in rainbow trout challenged with fliC gene–repressed (024) and fliC gene–expressing (R57) Yersinia ruckeri strains. The result is the average of two repetitions. L – low infectious dose; H – high infectious dose. Colour gradient corresponds to the mean number of individuals showing clinical symptoms

Fig. 2.

Mortality of rainbow trout challenged with fliC gene–repressed (024) and fliC gene–expressing (R57) Yersinia ruckeri strains. Mortality in the negative control group lies along the X-axis. L – low infectious dose; H – high infectious dose
Mortality of rainbow trout challenged with fliC gene–repressed (024) and fliC gene–expressing (R57) Yersinia ruckeri strains. Mortality in the negative control group lies along the X-axis. L – low infectious dose; H – high infectious dose

Fig. 3.

The humoral-mediated immune parameters in serum of rainbow trout challenged with fliC gene–repressed (024) and fliC gene–expressing (R57) Yersinia ruckeri strains. All data are expressed as mean + standard error of the mean. Different letters indicate significant differences (P-value < 0.05), shared or the same letters indicate no significant differences between groups
The humoral-mediated immune parameters in serum of rainbow trout challenged with fliC gene–repressed (024) and fliC gene–expressing (R57) Yersinia ruckeri strains. All data are expressed as mean + standard error of the mean. Different letters indicate significant differences (P-value < 0.05), shared or the same letters indicate no significant differences between groups

Fig. 4.

The cell-mediated immune parameters of rainbow trout challenged with fliC gene–repressed (024) and fliC gene–expressing (R57) Yersinia ruckeri strains. All data are expressed as stimulation index + standard error of the mean. Different letters indicate significant differences (P-value < 0.05), and shared or the same letters indicate no significant differences between groups
The cell-mediated immune parameters of rainbow trout challenged with fliC gene–repressed (024) and fliC gene–expressing (R57) Yersinia ruckeri strains. All data are expressed as stimulation index + standard error of the mean. Different letters indicate significant differences (P-value < 0.05), and shared or the same letters indicate no significant differences between groups

Multi-locus sequence typing (MLST) and core-genome MLST (cgMLST) for Yersinia ruckeri infection model strains

IsolateTyping schemeSequence typeHousekeeping genes in the scheme (reference)
Y. ruckeri 024PP2020Y. ruckeri R57MLST McNally (12)4444aarF (13)aarF (13)dfp (10)dfp (10)galR (14)galR (14)glnS (13)glnS (13)hemA (15)hemA (15)rfaE (14)rfaE (14)speA (14)speA (14)
Y ruckeri 024PP2020Y. ruckeri R57MLST Bastardo (4)3333dnaJ (1)dnaJ (1)glnA (1)glnA (1)gyrB(1)gyrB(1)hsp60 (1)hsp60 (1)recA (5)recA (5)thrA (5)thrA (5)--
Y ruckeri 024PP2020Y ruckeri R57cgMLST19271927 --

Experimental groups of rainbow trout challenged with fliC gene–repressed (024) and fliC gene–expressing (R57) Yersinia ruckeri strains

GroupBacterial strainInfectious dose (CFU/fish)nRepetitions
C--101
024-L024PP20202 × 105102
024-H024PP20202 × 106102
R57-LR572 × 105102
R57-HR572 × 106102
Language: English
Submitted on: Mar 5, 2025
Accepted on: Oct 16, 2025
Published on: Oct 22, 2025
Published by: National Veterinary Research Institute in Pulawy
In partnership with: Paradigm Publishing Services
Publication frequency: 4 issues per year

© 2025 Patrycja Schulz, Joanna Pajdak-Czaus, Karolina Pospiech, Elżbieta Fornal, Amanda Kobiera, Justyna Matczak, Paweł Foksiński, Andrzej Krzysztof Siwicki, published by National Veterinary Research Institute in Pulawy
This work is licensed under the Creative Commons Attribution 4.0 License.

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