Table 1.
Primers sequences used in the study
| Gene | Forward primer | Reverse primer | Expected product length (bp) |
|---|---|---|---|
| 16srRNA | AGGGAGCTTGCCTTGGATTC | GGATGCAGTTCCCAGGTTGA | 557 |
| gyrB | CCGGAGACCTTCAGCAACAT | TTGCCCATCTCCTGTTCCAC | 548 |
| rpoD | AAAGAGTTGATCGCCCGAGG | TGCTGTCGTCGCTTTCTTCT | 583 |
| rpoB | TTGTTTCTGTTGCTGCGTCG | GATTTCCTCTGGGCCAAGCT | 572 |
Fig. 1.
Electrophoretic separation of the PCR products of amplification of one small subunit and three housekeeping genes of bacteria isolated from a dog with periodontitis
1 – 16 srRNA gene product; 2 – gyr B1 gene product; 3 – rpo D1 gene product; 4 – rpo B1 gene product; M – 100 bp weight marker; NC – negative control
Table 2.
Results of BLAST used in bacterial isolate molecular identification
| Sequence | Query coverage | Percent identity | Length | Taxon | Accession No. |
|---|---|---|---|---|---|
| rpoB | 99% | 91% | 6,375,262 | Pseudomonas aeruginosa | CP046602.1 |
| rpoD | 97% | 99.81% | 6,375,262 | Pseudomonas aeruginosa | CP046602.1 |
| gyrB | 99% | 99.6% | 6,375,262 | Pseudomonas aeruginosa | CP046602.1 |
| 16srRNA | 100% | 99.42% | 1,490 | Pseudomonas aeruginosa | PP112146.1 |