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Is Pseudomonas aeruginosa a possible aetiological agent of periodontitis in dogs? Cover

Is Pseudomonas aeruginosa a possible aetiological agent of periodontitis in dogs?

Journal of Veterinary Research
Volume 69 (2025): Issue 1 (March 2025)
By: Małgorzata Targońska-Karasek,  Izabela Polkowska and  Henryk Krukowski  
Open Access
|Feb 2025

Figures & Tables

Fig. 1.

Electrophoretic separation of the PCR products of amplification of one small subunit and three housekeeping genes of bacteria isolated from a dog with periodontitis 1 – 16 srRNA gene product; 2 – gyr B1 gene product; 3 – rpo D1 gene product; 4 – rpo B1 gene product; M – 100 bp weight marker; NC – negative control
Electrophoretic separation of the PCR products of amplification of one small subunit and three housekeeping genes of bacteria isolated from a dog with periodontitis 1 – 16 srRNA gene product; 2 – gyr B1 gene product; 3 – rpo D1 gene product; 4 – rpo B1 gene product; M – 100 bp weight marker; NC – negative control

Primers sequences used in the study

GeneForward primerReverse primerExpected product length (bp)
16srRNAAGGGAGCTTGCCTTGGATTCGGATGCAGTTCCCAGGTTGA557
gyrBCCGGAGACCTTCAGCAACATTTGCCCATCTCCTGTTCCAC548
rpoDAAAGAGTTGATCGCCCGAGGTGCTGTCGTCGCTTTCTTCT583
rpoBTTGTTTCTGTTGCTGCGTCGGATTTCCTCTGGGCCAAGCT572

Results of BLAST used in bacterial isolate molecular identification

SequenceQuery coveragePercent identityLengthTaxonAccession No.
rpoB99%91%6,375,262Pseudomonas aeruginosaCP046602.1
rpoD97%99.81%6,375,262Pseudomonas aeruginosaCP046602.1
gyrB99%99.6%6,375,262Pseudomonas aeruginosaCP046602.1
16srRNA100%99.42%1,490Pseudomonas aeruginosaPP112146.1
DOI: https://doi.org/10.2478/jvetres-2025-0006 | Journal eISSN: 2450-8608
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Language: English
Page range: 35 - 40
Submitted on: Jul 3, 2024
Accepted on: Feb 7, 2025
Published on: Feb 25, 2025
Published by: National Veterinary Research Institute in Pulawy
In partnership with: Paradigm Publishing Services
Publication frequency: 4 issues per year
Keywords:
dog,
periodontitis
Related subjects:
Life sciences,
Molecular biology,
Microbiology and virology,
Life sciences, other,
Medicine,
Veterinary medicine

© 2025 Małgorzata Targońska-Karasek, Izabela Polkowska, Henryk Krukowski, published by National Veterinary Research Institute in Pulawy
This work is licensed under the Creative Commons Attribution 4.0 License.

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