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Proteomic insights into nematode-trapping fungi Arthrobotrys oligospora after their response to chitin Cover

Proteomic insights into nematode-trapping fungi Arthrobotrys oligospora after their response to chitin

Open Access
|Feb 2025

Figures & Tables

Fig. 1.

Comparison of the growth rate and sporulation capacity of mycelia in Arthrobotrys oligospora before and after chitin interaction. A and B – the colony morphology and its mycelial growth rate; C and D – sporulation ability. AO-c – control group; AO-e – experimental group
Comparison of the growth rate and sporulation capacity of mycelia in Arthrobotrys oligospora before and after chitin interaction. A and B – the colony morphology and its mycelial growth rate; C and D – sporulation ability. AO-c – control group; AO-e – experimental group

Fig. 2.

Comparison of morphology, predation structure and predation ability of spores in Arthrobotrys oligospora before and after chitin interaction. A – spore morphology; B – predation structure; C and D – predation ability on nematodes. AO-c – control group; AO-e – experimental group
Comparison of morphology, predation structure and predation ability of spores in Arthrobotrys oligospora before and after chitin interaction. A – spore morphology; B – predation structure; C and D – predation ability on nematodes. AO-c – control group; AO-e – experimental group

Fig. 3.

Gene Ontology enrichment analysis of differentially expressed proteins in Arthrobotrys oligospora before and after chitin interaction
Gene Ontology enrichment analysis of differentially expressed proteins in Arthrobotrys oligospora before and after chitin interaction

Fig. 4.

Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis of differentially expressed proteins (DEPS) in Arthrobotrys oligospora before and after chitin interaction. A – KEGG pathway enrichment category of DEPs; B – bubble map of DEPs involved in metabolic pathways in KEGG analysis
Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis of differentially expressed proteins (DEPS) in Arthrobotrys oligospora before and after chitin interaction. A – KEGG pathway enrichment category of DEPs; B – bubble map of DEPs involved in metabolic pathways in KEGG analysis

Fig. 5.

Network diagram analysis of differentially expressed proteins involved in metabolic pathways in Arthrobotrys oligospora before and after chitin interaction
Network diagram analysis of differentially expressed proteins involved in metabolic pathways in Arthrobotrys oligospora before and after chitin interaction

Fig. 6.

Determination of the degradation activity of differentially expressed chitinase AO-379 in Caenorhabditis elegans. A–C – C. elegans treated with inactivated ReAO-379 for 0, 6 and 12 h, respectively; D–F – adult C. elegans treated with ReAO-379 for 0, 6 and 12 h, respectively; G–I – stage-IV larvae of C. elegans treated with ReAO-379 for 0, 6 and 12 h, respectively. Arrow – degraded part of the nematode body wall; scale bar – 50 μm
Determination of the degradation activity of differentially expressed chitinase AO-379 in Caenorhabditis elegans. A–C – C. elegans treated with inactivated ReAO-379 for 0, 6 and 12 h, respectively; D–F – adult C. elegans treated with ReAO-379 for 0, 6 and 12 h, respectively; G–I – stage-IV larvae of C. elegans treated with ReAO-379 for 0, 6 and 12 h, respectively. Arrow – degraded part of the nematode body wall; scale bar – 50 μm

Fig. 7.

Analysis of the degradation activity of differentially expressed chitinase AO-379 in Caenorhabditis elegans eggs. A–C – C. elegans eggs treated with inactivated recombinant ReAO-379 for 0, 6 and 12 h, respectively; D–F – C. elegans eggs treated with ReAO-379 for 0, 6 and 12 h, respectively. Arrow – degraded part of egg shell; scale bar – 100 μm
Analysis of the degradation activity of differentially expressed chitinase AO-379 in Caenorhabditis elegans eggs. A–C – C. elegans eggs treated with inactivated recombinant ReAO-379 for 0, 6 and 12 h, respectively; D–F – C. elegans eggs treated with ReAO-379 for 0, 6 and 12 h, respectively. Arrow – degraded part of egg shell; scale bar – 100 μm

The top 10 proteins with differentially downregulated expression among non-intersection proteins of Arthrobotrys oligospora after chitin interaction

Protein IDProtein nameGene nameScore
A0A7C8UKJ8GH16 domain-containing proteinAOL_s00007g459323.31
A0A7C8N8K3Ribonuclease II/R domain-containing proteinAOL_s00173g295323.31
G1XD83Sla2 Src-like adaptor 2AOL_s00079g186303.54
G1XIU1Glycosyl transferase family 1 domain-containing proteinAOL_s00097g268275.82
A0A7C8NE69Aminotransferase class V domain-containing proteinTWF102_003885239.24
G1XCR6Hsp70 protein that interacts with Zuo1pAOL_s00079g236231.82
A0A6G1M536Uncharacterised proteinAOL_s00083g273226.37
A0A7C8P375Tubulin alpha chainAOL_s00078g243224.51
A0A7C8UJ533-hydroxyacyl-CoA dehydrogenase NAD binding domain-containing proteinAOL_s00110g113219.76
G1WXJ7Uracil phosphoribosyl transferaseAOL_s00004g7198.86

The top 10 proteins with differentially upregulated expression among non-intersection proteins of Arthrobotrys oligospora after chitin interaction

Protein IDProtein nameGene nameScore
G1X8M1Ubiquitin-like domain-containing proteinAOL_s00078g37323.31
A0A8H8UWC8V-type proton ATPase subunitAOL_s00109g205313.43
A0A7C8N5S6Superoxide dismutaseAOL_s00170g93277.7
G1XBG1Ribosomal protein S15AOL_s00078g258266.86
G1XJC0AA1-like domain-containing proteinAOL_s00097g447173.69
A0A7C8KEK2Copper acquisition factor BIM1-like domain-containing proteinEYR41_002241147.12
A0A8H2E0I4Neutral ceramidaseAOL_s00076g455141.71
A0A7C8NSM9Uncharacterised proteinAOL_s00076g540109.37
G1XJ38Ribosomal protein S7 domain-containing proteinAOL_s00097g36599.319
G1WY88SAC domain-containing proteinAOL_s00004g24872.201

Gene Ontology molecular function categories that were most significantly enriched in differentially expressed proteins in Arthrobotrys oligospora after chitin interaction

LevelGene Ontology nameGene Ontology IDP-valueCount
2catalytic activityGO:00038245.78E–17554
4anion bindingGO:00431681.01E–10232
3small molecule bindingGO:00360944.36E–09239
4oxidoreductase activity, acting on CH-OH group of donorsGO:00166144.14E–0828
3carbohydrate derivative bindingGO:00973678.13E–08184
4nucleotide bindingGO:00001660.000000215216
4nucleoside phosphate bindingGO:19012650.000000215216
4ribonucleotide bindingGO:00325530.000000422178
5purine ribonucleoside triphosphate bindingGO:00356390.00000052173
4nucleoside bindingGO:00018820.000000604170

The top 10 proteins with differentially upregulated expression among intersection proteins of Arthrobotrys oligospora after chitin interaction

Protein IDProtein nameGene nameFold change
A0A7C8NPA7ChitinaseAOL_s00006g49225.19742557
G1XLE5Peptide hydrolaseAOL_s00110g31513.27323346
A0A7C8UMP1Beta-hexosaminidaseAOL_s00080g28611.49963777
A0A7C8PQM7Aminotransferase class I/class II domain-containing proteinAOL_s00004g2647.175107854
A0A7C8NRM1FAD dependent oxidoreductase domain-containing proteinAOL_s00117g576.570614843
G1XG12Aminotransferase class I/class II domain-containing proteinAOL_s00081g3055.443764901
A0A7C8NZY3AA1-like domain-containing proteinAOL_s00043g1414.887246226
A0A7C8JS84Urease (fragment)AOL_s00080g264.05366717
A0A7C8K8Z4Glucose-methanol-choline oxidoreductase C-terminal domain-containing proteinAOL_s00006g5714.050636221
G1X3Q8Glucan 1,4-alpha-glucosidaseAOL_s00043g3323.844588344

The top 10 proteins with differentially downregulated expression among intersection proteins of Arthrobotrys oligospora after chitin interaction

Protein IDProtein nameGene nameFold change
A0A7C8KHY8Translation elongation factor IF5A C-terminal domain-containing proteinAOL_s00112g560.261838199
A0A7C8N2H5kinase domain-containing proteinAOL_s00079g2540.246729316
A0A7C8PAR5Vacuolar protease AAOL_s00054g4780.24519951
G1XG2060S ribosomal protein L3AOL_s00081g3130.238139381
A0A7C8V39360S ribosomal protein L17AOL_s00097g4110.226801505
G1XMA1Uncharacterised proteinAOL_s00140g700.210533753
A0A8H2DUD7Apple domain-containing proteinAOL_s00017g640.174564146
G1XEX5PBP domain-containing proteinAOL_s00080g3600.157891296
A0A7C8R9J6Phosphatidylserine decarboxylaseAOL_s00097g760.150443331
A0A7C8NXE9Translation elongation factor 1-alpha (fragment)AOL_s00169g480.135301915
Language: English
Page range: 71 - 82
Submitted on: Jul 3, 2024
Accepted on: Feb 3, 2025
Published on: Feb 25, 2025
Published by: National Veterinary Research Institute in Pulawy
In partnership with: Paradigm Publishing Services
Publication frequency: 4 times per year

© 2025 Jiahua Zhang, Lixiang Wei, Huimei Zhang, Xixi Ma, Yansen Sun, Ruobing Li, Chengzhi Zhang, Xuepeng Cai, Jun Qiao, Qingling Meng, published by National Veterinary Research Institute in Pulawy
This work is licensed under the Creative Commons Attribution 4.0 License.