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Fig. 7.
Primers used in the reverse-transcriptase quantitative PCR to amplify Dirofilaria repens macrophage migration inhibitory factor (Dre-mif)-1 and -2
| Primer | Sequence |
|---|---|
| Dre-mif-1_F | 5′GGCTGATGAACT CAAAAT CCC 3′ |
| Dre-mif-1_R | 5′ACCCATTGCCGAAGCACTAATA 3′ |
| Dre-mif-2_F | 5′GATTGGATCATTTTCGGCTGATA 3′ |
| Dre-mif-2_R | 5′CGTACCATTGCATCCCACATTT 3′ |
The protein identity between human macrophage migration macrophage migration inhibitory factor (hMIF), dog MIF (dMIF), Dirofilaria repens (Dre)-MIF-1 and Dre-MIF-2
| Protein | hMIF | dMIF | Dre-MIF-1 | Dre-MIF-2 |
|---|---|---|---|---|
| hMIF | - | 93.9% | 41.7% | 27.8% |
| dMIF | 93.9% | - | 40.0% | 26.9% |
| Dre-MIF-1 | 41.7% | 40.0% | - | 27.8% |
| Dre-MIF-2 | 27.8% | 26.9% | 27.8% | - |