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Effect on the splenocyte function of weaned piglets induced by continuous lipopolysaccharide injections Cover

Effect on the splenocyte function of weaned piglets induced by continuous lipopolysaccharide injections

Journal of Veterinary Research
Volume 68 (2024): Issue 2 (June 2024)
By: Tingyu Yang,  Guotong Zhao,  Wenlu Zhu,  Wanting Yu,  Yijie Jiang,  Yunxiao Zhou and  Yong Li  
Open Access
|May 2024
Figures & tables

Figures & Tables

Fig. 1.

Effect on splenocyte proliferation in weaned piglets induced by lipopolysaccharide (LPS) challenge. Data represent the mean ± standard deviation (n = 6 in each group) ** – highly significant difference (P-value < 0.01)

Fig. 2.

Flow cytometry results of evaluation of weaned piglet splenocyte cell cycle distribution after lipopolysaccharide (LPS) challenge. A, B, C and D show the splenocytes of saline-treated piglets on day (d)1, d5, d9 and d13, respectively; a, b, c and d show the splenocytes of LPS-treated piglets on the same days; G0 – cells in resting phase; G1 – cells in gap 1 phase; S – cells in synthesis phase; G2 – cells in gap 2 phase; M – cells in mitosis phase; FL2-A – total relative fluorescent intensity in channel 2; PI-A – total fluorescent intensity from propidium iodide

Fig. 3.

Effect of lipopolysaccharide (LPS) challenge to weaned piglets on the cell cycle distribution and proliferation index (PrI) of splenocytes. A – percentage of splenocytes in the resting phase (G0) and gap 1 phase (G1); B – percentage of splenocytes in the synthesis (S), gap 2 (G2) and mitosis (M) phases; C – cell cycle phase distribution of splenocytes (NC – negative control group); D – Proliferation index. Data represent the mean ± standard deviation (n = 6 in each group) ** – highly significant difference (P-value < 0.01)

Fig. 4.

Percentages of apoptosis in splenocytes detected using Annexin V-enhanced green fluorescent protein (EGFP)/propidium iodide staining by flow cytometry. Apoptosis was affected by lipopolysaccharide (LPS) challenge to weaned piglets. A, B, C and D show the splenocytes of saline-treated piglets on day (d)1, d5, d9 and d13, respectively; a, b, c and d show the splenocytes of LPS-treated piglets on the same days; Q – quadrant; Comp – compensated; FL1/2-A – total relative fluorescent intensity in channel 1/2; PI-A – total fluorescent intensity from propidium iodide; EGFP-A – total fluorescent intensity from enhanced green fluorescent protein

Fig. 5.

Effect of lipopolysaccharide (LPS) challenge to weaned piglets on the percentage of apoptosis in splenocytes. Data represent the mean ± standard deviation (n = 6 in each group) ** – highly significant difference (P-value < 0.01)

Fig. 6.

Effect of lipopolysaccharide (LPS) challenge to weaned piglets on the nitric oxide secretion in splenocytes. Data represent the mean ± standard deviation (n = 6 in each group) ** – highly significant difference (P-value < 0.01)
DOI: https://doi.org/10.2478/jvetres-2024-0024 | Journal eISSN: 2450-8608
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Language: English
Page range: 295 - 302
Submitted on: Oct 28, 2023
Accepted on: Apr 22, 2024
Published on: May 9, 2024
Published by: National Veterinary Research Institute in Pulawy
In partnership with: Paradigm Publishing Services
Publication frequency: 4 issues per year
Keywords:
weaned piglet,
LPS,
splenocytes,
immune function
Related subjects:
Life sciences,
Molecular biology,
Microbiology and virology,
Life sciences, other,
Medicine,
Veterinary medicine

© 2024 Tingyu Yang, Guotong Zhao, Wenlu Zhu, Wanting Yu, Yijie Jiang, Yunxiao Zhou, Yong Li, published by National Veterinary Research Institute in Pulawy
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Volume 68 (2024): Issue 2 (June 2024)
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