Fig. 1.
Detailed clinical information on cats from central Slovakian animal shelters or veterinary clinics with signs of feline panleukopenia
| Group | Sample ID | Breed | Sex | Age | FPV vaccination | Anamnesis |
|---|---|---|---|---|---|---|
| I. clinical signs (n = 21) | 1 | crossbreed | female | 3 months | no | vomiting, diarrhoea |
| 2 | crossbreed | male | 3 months | no | vomiting, diarrhoea, lethargy | |
| 3 | crossbreed | female | 2 months | no | vomiting, diarrhoea, fever | |
| 4 | ESH | female | 6 months | no | lethargy, anorexia, fever | |
| 5 | ESH | female | 5 months | no | lethargy, anorexia | |
| 6 | crossbreed | female | 2 months | no | vomiting, diarrhoea | |
| 7 | crossbreed | male | 3 months | no | vomiting, lethargy | |
| 8 | crossbreed | female | 3 months | no | lethargy, fever | |
| 9 | crossbreed | female | 3 months | no | vomiting, diarrhoea, fever | |
| 10 | ESH | female | 3 years | no | lethargy, fever | |
| 11 | crossbreed | female | 4 months | yes | vomiting, lethargy, fever | |
| 12 | ESH | female | 3 years | no | vomiting, lethargy, anorexia | |
| 13 | crossbreed | male | 7 months | no | vomiting, lethargy, fever | |
| 14 | crossbreed | male | 6 months | no | diarrhoea, fever, vomiting | |
| 15 | crossbreed | male | 5 years | yes | diarrhoea | |
| 16 | ESH | male | 3 months | no | vomiting, lethargy, fever | |
| 17 | ESH | male | 5 months | no | vomiting, lethargy, fever, diarrhoea | |
| 18 | crossbreed | male | 5 months | no | vomiting, lethargy, fever | |
| 19 | ESH | male | 6 months | no | vomiting, lethargy, fever | |
| 20 | crossbreed | male | 1 year | no | vomiting, diarrhoea | |
| 21 | crossbreed | male | 3 months | no | diarrhoea, vomiting | |
| II. close contact with parvovirus-infected animal (n = 29) | 1 | crossbreed | female | 4 years | yes | healthy |
| 2 | crossbreed | female | 6 months | no | healthy | |
| 3 | crossbreed | male | 4 months | no | healthy | |
| 4 | crossbreed | female | 4 years | yes | healthy | |
| 5 | crossbreed | female | 5 years | yes | healthy | |
| 6 | ESH | female | 2 years | no | healthy | |
| 7 | ESH | female | 1 year | no | healthy | |
| 8 | ESH | female | 4 years | yes | healthy | |
| 9 | crossbreed | male | 5 years | yes | healthy | |
| 10 | ESH | female | 4 months | no | diarrhoea | |
| 11 | crossbreed | female | 3 months | no | diarrhoea | |
| 12 | crossbreed | male | 2 months | no | diarrhoea | |
| 13 | crossbreed | female | 3 months | no | diarrhoea, vomiting | |
| 14 | crossbreed | male | 3 months | no | healthy | |
| 15 | ESH | female | 1 year | no | healthy | |
| 16 | crossbreed | male | 5 months | no | healthy | |
| 17 | crossbreed | male | 2 years | yes | healthy | |
| 18 | crossbreed | male | 2 years | yes | intermittent diarrhoea | |
| 19 | crossbreed | female | 5 months | no | diarrhoea, overcame FPL | |
| 20 | ESH | female | 5 years | no | healthy | |
| 21 | crossbreed | male | 2 years | yes | intermittent diarrhoea | |
| 22 | crossbreed | female | 4 months | no | healthy | |
| 23 | ESH | female | 2 years | no | healthy | |
| 24 | crossbreed | female | 1 year | no | healthy, overcame FPL | |
| 25 | crossbreed | male | 1 year | yes | healthy | |
| 26 | crossbreed | female | 5 months | no | diarrhoea, overcame FPL | |
| 27 | crossbreed | male | 4 months | no | vomiting | |
| 28 | crossbreed | female | 3 years | yes | healthy | |
| 29 | crossbreed | female | 6 months | no | healthy, overcame FPL | |
| III. clinically healthy (n = 9) | 1 | ESH | male | 1 year | yes | healthy |
| 2 | ESH | female | 2 years | yes | healthy | |
| 3 | crossbreed | male | 7 months | yes | healthy | |
| 4 | crossbreed | female | 3 years | yes | healthy | |
| 5 | crossbreed | male | 3 months | yes | healthy | |
| 6 | crossbreed | male | 5 months | yes | healthy | |
| 7 | crossbreed | female | 1 year | yes | healthy | |
| 8 | crossbreed | female | 2 years | yes | healthy | |
| 9 | ESH | male | 1 year | yes | healthy |
Antigen-based detection of parvovirus infection in rectal swab samples of European shorthair and crossbred cats in central Slovakia grouped by feline panleukopenia status
| Group | Sample ID | CPV antigen test |
|---|---|---|
| I. clinical signs (n = 21) | 1–21 | + |
| II. close contact with parvovirus-infected animal (n = 29) | 1–9; 11–26; 28, 29 | - |
| 10, 27 | + | |
| III. clinically healthy (n = 9) | 1–9 | - |
Summary of parvovirus infection findings in rectal swab samples of European shorthair and crossbred cats in central Slovakia grouped by feline panleukopenia status
| Groups | CPV antigen test | PCR test | Total positive | ||
|---|---|---|---|---|---|
| Ag+ | Ag- | PCR+ | PCR- | ||
| I. clinical signs (n = 21) | 21 | 0 | 21 | 0 | 21 (100%) |
| II. close contact with parvovirus-infected animals (n = 29) | 2 | 27 | 2 | 27 | 2 (6.89%) |
| III. clinically healthy (n = 9) | 0 | 9 | 0 | 9 | 0 |
Sequences producing significant alignments with the VP2 gene amplicons of investigated rectal swab samples from European shorthair and crossbred cats in central Slovakia_ Sequence analysis used the National Center for Biotechnology Information BLASTn algorithm
| Scientific name | Pairwise identity (%) | Query coverage (%) | GenBank accession number |
|---|---|---|---|
| Feline panleukopenia virus | 100.00 | 100 | MK671185.1, MK671183.1, MK671171.1, MK671170.1, MK671156.1, MK425504.1, MK425500.1, MK425499.1, MK425498.1, MK425497.1, KP019621.2, MG764511.1, MG764510.1, MF541133.1, MF541132.1, MF541119.1, KX685354.1, KX900570.1, KT240130.1, KT357491.1, OP796716.1, OP796714.1, KP682520.1, KP019617.1, OM638042.1, MW091486.1, MW091487.1, MZ712026.1, MZ322607.1, MZ357122.1, MZ357120.1, MZ357119.1, MW017627.1, MT270583.1, MW331496.1, MN419003.1, MT274378.1, HQ184201.1, FJ936171.1, DQ474238.1, DQ474237.1, DQ474236.1, EU221281.1, AY606131.1, DQ099430.1, AY955826.1, DQ003301.1, AB054227.1, AB054226.1, AB054225.1 |
| Feline parvovirus | 100.00 | 100 | MK266790.1, MK266788.1, MK295775.1, OM885375.1, ON646210.1, MZ442303.1, OR211675.1 |
| Feline panleukopenia virus | 99.78 | 100 | OQ863619.1, OQ863618.1, OQ863617.1, OQ863615.1, MZ508524.1 |
| Feline parvovirus | 99.78 | 100 | OR198066.1, OQ869254.1, OQ868569.1, OQ868568.1, OQ868566.1, OQ868565.1, OQ868564.1, OQ868562.1, OQ868554.1, OQ868553.1, OQ868552.1, OQ868550.1, OQ868548.1, OQ868547.1, OQ868542.1, OQ868536.1, OQ868535.1, OQ868534.1, OR194134.1, OR194132.1, OR194130.1, OR194129.1, OR194128.1, OR194125.1, OR194122.1, OQ535514.1, OQ535513.1, OQ535511.1, OQ535510.1, OQ535509.1 |