Protective effects of N-acetyl-l-cysteine against penconazole-triggered hepatorenal toxicity in adult rats

Ashraf M. Morgan; Hanan A. Ogaly; Shaimaa Kamel; Maha M. Rashad; Eman I. Hassanen; Marwa A. Ibrahim; Mona K. Galal; Aya M. Yassin; Sharah A. Al Dulmani; Fatimah A.M. Al-Zahrani; Ahmed M. Hussien

doi:10.2478/jvetres-2023-0039

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Protective effects of N-acetyl-l-cysteine against penconazole-triggered hepatorenal toxicity in adult rats

Journal of Veterinary Research

Volume 67 (2023): Issue 3 (September 2023)

By: Ashraf M. Morgan, Hanan A. Ogaly, Shaimaa Kamel, Maha M. Rashad, Eman I. Hassanen, Marwa A. Ibrahim, Mona K. Galal, Aya M. Yassin, Sharah A. Al Dulmani, Fatimah A.M. Al-Zahrani and Ahmed M. Hussien

Open Access

|Sep 2023

Abstract

Introduction

Penconazole (PEN) is a widely applied triazole fungicide. This study sought to define the efficacy of N-acetyl-l-cysteine (NAC) in mitigating PEN-triggered hepatorenal toxicity in rats.

Material and Methods

Twenty-eight adult male albino Wistar rats were assigned to four groups: a normal control (NC), a PEN group, a NAC group and a PEN+NAC group. Administration of PEN (50 mg/kg body weight (b.w.) every 2 days) and NAC (150 mg/kg b.w., daily) took place via oral gavage for 10 days.

Results

Effective amelioration by NAC of PEN-induced liver and kidney dysfunction was indicated by a significant reduction in the circulating liver and kidney markers (aspartate aminotransferase, alanine aminotransferase, urea and creatinine). Attenuation of PEN-induced oxidative stress and lipid peroxidation in liver and kidney tissues was evident in a significant reduction in malondialdehyde and enhanced total antioxidant capacity. Moreover, NAC significantly reduced the histopathological alterations and the expression of tumour necrosis factor α in liver and kidney tissue. Furthermore, NAC maintained the messenger RNA levels of nuclear factor erythroid 2-related factor 2 (Nrf2), haem oxygenase 1, and Kelch-like erythroid cell-derived protein 1 and prevented nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) protein upregulation caused by PEN.

Conclusion

N-acetyl-1-cysteine protected against PEN-induced hepatorenal oxidative damage and inflammatory response via activation of Nrf2 and inhibition of NF-κB pathways.

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Articles in this issue

DOI: https://doi.org/10.2478/jvetres-2023-0039 | Journal eISSN: 2450-8608

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Language: English

Page range: 459 - 469

Submitted on: Dec 24, 2022

Accepted on: Jun 26, 2023

Published on: Sep 20, 2023

Published by: National Veterinary Research Institute in Pulawy

In partnership with: Paradigm Publishing Services

Keywords:

penconazole,

Nrf2,

HO-1,

Keap1,

NF-κB

Related subjects:

Life sciences,

Molecular biology,

Microbiology and virology,

Life sciences, other,

Medicine,

Veterinary medicine

© 2023 Ashraf M. Morgan, Hanan A. Ogaly, Shaimaa Kamel, Maha M. Rashad, Eman I. Hassanen, Marwa A. Ibrahim, Mona K. Galal, Aya M. Yassin, Sharah A. Al Dulmani, Fatimah A.M. Al-Zahrani, Ahmed M. Hussien, published by National Veterinary Research Institute in Pulawy
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Volume 67 (2023): Issue 3 (September 2023)