Table 1
Red deer blood complete blood count and serum biochemistry panel on the 50th day after being mated (October) and 40 days before planned parturition (April)
| Parameter | First collection (October) (n = 30) | Second collection (April) (n = 29) |
|---|---|---|
| RBC (1012/L) | 10.52 ± 1.80 | 11.53 ± 1.92 |
| HGB (g/L) | 16.19 ± 2.11a | 17.77 ± 2.63a |
| HCT (L/L) | 0.43 ± 0.05a | 0.54 ± 0.08a |
| MCV (fL) | 41.72 ± 4.98a | 47.00 ± 4.10a |
| MCH (pg) | 15.56 ± 1.45 | 15.50 ± 1.22 |
| MCHC (g/dL) | 37.52 ± 1.30a | 33.25 ± 0.80a |
| WBC (109/L) | 6.20 ± 1.80 | 5.35 ± 1.74 |
| LYM (109/L) | 3.50 ± 1.04 | 3.11 ± 1.17 |
| MID (109/L) | 0.41 ± 0.28 | 0.42 ± 0.20 |
| GRA (109/L) | 2.30 ± 1.07 | 1.82 ± 1.12 |
| PLT (109/L) | 211.07 ± 69.69 | 212.57 ± 85.40 |
| MPV (fL) | 6.01 ± 0.52 | 5.90 ± 0.36 |
| Total protein (g/L) | 66.00 ± 4.44a | 73.07 ± 8.96a |
| Albumin (g/L) | 37.17 ± 3.72 | 39.47 ± 8.49 |
| Cholesterol (mmol/L) | 1.82 ± 0.34 | 1.83 ± 0.31 |
| HDL (mmol/L) | 1.25 ± 0.23 | 1.20 ± 0.22 |
| LDL (mmol/L) | 0.62 ± 0.19a | 0.47 ± 0.20a |
| Triglyceride (mmol/L) | 0.20 ± 0.11a | 0.15 ± 0.08a |
[i] RBC – red blood cells; HGB – haemoglobin; HCT – haematocrit; MCV – mean corpuscular volume; MCH – mean cell haemoglobin; MCHC – mean cell haemoglobin concentration; WBC – white blood cell count; LYM – lymphocyte; MID – monocyte; GRA – granulocyte; PLT – platelet count; MPV – mean platelet volume; HDL – high-density lipoprotein; LDL – low-density lipoprotein Values are mean ±SD, a indicates significant differences (P < 0.05)
Table 2
Blood biomarker concentrations in red deer blood on the 50th day after being mated (October) and 40 days before planned parturition (April)
| First collection | Second collection | |
|---|---|---|
| Parameter | (October) | (April) |
| (n = 30) | (n = 29) | |
| Serum | ||
| SOD (U/mL) | 21.96 ± 6.70 | 20.01 ± 5.62 |
| CAT (U/mL) | 50.33 ± 19.23a | 27.53 ± 15.15a |
| GR (U/L) | 160.58 ± 82.74a | 224.29 ± 71.94a |
| PON(U/mL) 1 (paraoxonase) | 239.60 ± 62.19 | 242.63 ± 61.96 |
| PON(U/mL) 1 (arylesterase) | 171.57 ± 36.25 | 186.53 ± 52.90 |
| PON(U/mmoL) 1/HDL ratio | 207.27 ± 65.30 | 200.13 ± 61.16 |
| TAS (mmol/L) | 0.71 ± 0.25 | 0.79 ± 0.14 |
| total (μmol/bilirubin L) | 5.99 ± 3.25 | 6.70 ± 3.11 |
| uric acid (mmol/L) | 0.02 ± 0.01 | 0.02 ± 0.03 |
| Haemolysate | ||
| SOD (U/mL) | 256.34 ± 66.44 | 292.27 ± 107.24 |
| SOD (U/g Hb) | 1608.82 ± 462.90 | 1676.88 ± 611.50 |
| total (μmol/glutathione L) | 71.16 ± 30.67a | 94.91 ± 39.89a |
| GSSG (μmol/L) | 59.31 ± 23.64a | 73.96 ± 30.37a |
| Plasma/serum | ||
| MDA in plasma | 1.38 ± 0.35a | 1.19 ± 0.31a |
| BHB in serum (mmol/L) | 0.26 ± 0.07a | 0.43 ± 0.12a |
[i] SOD – superoxide dismutase; CAT – catalase; GR – glutathione reductase; PON1 – PON1 paraoxonase 1; HDL – high-density lipoprotein; TAS – total antioxidant status; GSSG – glutathione disulphide; MDA – malondialdehyde; BHB – beta-hydroxybutyrate Values are mean ±SD, a indicates significant differences (P < 0.05)