Haematological and chosen biochemical parameter assessment of the antioxidant system in red deer (Cervus elaphus) blood in early and late pregnancy

Kulka, Marek; Kołodziejska-Lesisz, Joanna; Bartosik, Justyna; Klockiewicz, Maciej; Kluciński, Włodzimierz

doi:10.2478/jvetres-2022-0044

Abstract

Introduction

The aim of this study was to investigate changes in haematological parameters and the antioxidant system in the early and late pregnancy of red deer (Cervus elaphus).

Material and Methods

Blood samples were collected from 30 red deer females 50 days after impregnation and 40 days before calving. Complete blood counts and stained blood smears were assessed. Paraoxonase 1 (PON1), superoxide dismutase (SOD), catalase (CAT), glutathione reductase (GR) activities, glutathione disulphide (GSSG), total glutathione, total bilirubin, total protein, albumin, uric acid, malondialdehyde (MDA), beta-hydroxybutyrate (BHB), low-density lipoprotein (LDL), high-density lipoprotein (HDL), triglycerides, cholesterol levels and total antioxidant status (TAS) were measured.

Results

The haematological characteristics of blood collected in the third trimester showed higher haemoglobin levels, haematocrit, mean corpuscular volume (P < 0.05) and a lower mean cell haemoglobin concentration (P < 0.05) in comparison to samples collected on the 50^th day after mating. Activity of CAT and MDA, LDL, and triglyceride levels were lower while GR, GSSG, total glutathione, total protein and BHB levels were increased in blood samples drawn in the third trimester compared with the first trimester blood samples. There were no changes in SOD, PON1 activities, TAS, bilirubin, albumin, HDL, cholesterol or uric acid concentrations.

Conclusion

Red deer’s compensatory mechanisms facilitate its optimal adaptation to seasonal changes evidenced by mild pronounced haematological disturbances and an effective antioxidant system during pregnancy.

References

Arnold W., Beiglböck C., Burmester M., Guschlbauer M., Lengauer A., Schröder B., Wilkens M., Breves G.: Contrary seasonal changes of rates of nutrient uptake, organ mass, and voluntary food intake in red deer (Cervus elaphus). Am J Physiol Regul Integr Comp Physiol 2015, 309, R277–285, doi: 10.1152/ajpregu.00084.2015.
Open DOI Search in Google Scholar Back to article
Asher G.W., Scott I.C., Archer J.A., Ward J.F., Littlejohn R.P.: Seasonal luteal cyclicity of pubertal and adult red deer (Cervus elaphus). Anim Reprod Sci 2011, 125, 138–147, doi: 10.1016/j.anireprosci.2011.03.009.
Open DOI Search in Google Scholar Back to article
Aytekin I., Aksit H., Sait A., Kaya F., Aksit D., Gokmen M., Baca A.U.: Evaluation of oxidative stress via total antioxidant status, sialic acid, malondialdehyde and RT-PCR findings in sheep affected with bluetongue. Vet Rec Open 2015, 2, e000054, doi: 10.1136/vetreco-2014-000054.
Open DOI Search in Google Scholar Back to article
Barić-Rafaj R., Tončić J, Vicković I, Šoštarić B.: Haematological and biochemical values of farmed red deer (Cervus elaphus). Veterinarski Arhiv 2011, 81, 513–523.
Search in Google Scholar Back to article
Barrell G.K., Ridgway M.J., Wellby M., Pereira A., Henry B.A., Clarke I.J.: Expression of regulatory neuropeptides in the hypothalamus of red deer (Cervus elaphus) reveals anomalous relationships in the seasonal control of appetite and reproduction. Gen Comp Endocrinol 2016, 229, 1–7, doi: 10.1016/j.ygcen.2016.02.020.
Open DOI Search in Google Scholar Back to article
Bauchart D.: Lipid absorption and transport in ruminants. J Dairy Sci 1993, 76, 3864–3881, doi: 10.3168/jds.S0022-0302(93) 77728-0.
Open DOI Search in Google Scholar Back to article
Bell A.W.: Regulation of organic nutrient metabolism during transition from late pregnancy to early lactation. J Anim Sci 1995, 73, 2804–2819, doi: 10.2527/1995.7392804x.
Open DOI Search in Google Scholar Back to article
Bielecka A., Jamioł M., Kankofer M.: Antioxidative and oxidative profiles in plasma and saliva in dairy cows during pregnancy. Animals 2021, 11, 3204, doi: 10.3390/ani11113204.
Open DOI Search in Google Scholar Back to article
Bionaz M., Trevisi E., Calamari L., Librandi F., Ferrari A., Bertoni G.: Plasma paraoxonase, health, inflammatory conditions, and liver function in transition dairy cows. J Dairy Sci 2007, 90, 1740–1750, doi: 10.3168/jds.2006-445.
Open DOI Search in Google Scholar Back to article
Camargo C.M.S., Duarte J.M.B., Fagliari J.J., Santana A.M., Simplício K.M.M.G., Santana A.E.: Effect of sex and seasons of the year on hematologic and serum biochemical variables of captive brown brocket deer (Mazama gouazoubira). Pesq Vet Bras 2013, 33, 1364–1370, doi: 10.1590/S0100-736X2013001100013.
Open DOI Search in Google Scholar Back to article
Castillo C., Hernandez J., Bravo A., Lopez-Alonso M., Pereira V., Benedito J.L.: Oxidative status during late pregnancy and early lactation in dairy cows. Vet J 2005, 169, 286–292, doi: 10.1016/j.tvjl.2004.02.001.
Open DOI Search in Google Scholar Back to article
Chapple R.S., English A.W., Mulley R.C., Lepherd E.E.: Haematology and serum biochemistry of captive unsedated chital deer (Axis axis) in Australia. J Wildl Dis 1991, 27, 396–406, doi: 10.7589/0090-3558-27.3.396.
Open DOI Search in Google Scholar Back to article
Christie W.W.: The composition, structure and function of lipids in the tissues of ruminant animals. In: Lipid Metabolism in Ruminant Animals, edited by W.W. Christie, Pergamon, Oxford, 1981, pp. 125–130.
Search in Google Scholar Back to article
DelGiudice G.D., Mech L.D., Kunkel K.E., Gese E.M., Seal U.S.: Seasonal patterns of weight, hematology, and serum characteristics of free-ranging female white-tailed deer in Minnesota. Can J Zool 1992, 70, 974–983, doi: 10.1139/z92-139.
Open DOI Search in Google Scholar Back to article
Ditchkoff S.S.: Chapter 2, Anatomy and Physiology. In: Biology and management of white-tailed deer, edited by D.G. Hewitt, CRC Press, Boca Raton, FL, 2011, pp 59–62, doi: 10.1201/9781482295986.
Open DOI Search in Google Scholar Back to article
Hempstock J., Jauniaux E., Greenwold N., Burton, G.J.: The contribution of placental oxidative stress to early pregnancy failure. Hum Pathol 2003, 34, 1265–1275, doi: 10.1016/j.humpath.2003.08.006.
Open DOI Search in Google Scholar Back to article
Kulka M., Bełtowski J., Kluciński W., Orłowska M., Kołodziejska J., Kleczkowski M.: Serum paraoxonase-1 activity of dairy Holstein-Fresian cows in different lactation stages – preliminary study. Pol J Vet Sci 2014, 17, 143–147, doi: 10.2478/pjvs-2014-0019.
Open DOI Search in Google Scholar Back to article
Kulka M., Kołodziejska-Lesisz J., Kluciński W.: Serum paraoxonase 1 (PON1) activity and lipid metabolism parameters changes in different production cycle periods of Holstein-Friesian, Polish Red and Norwegian breeds. Pol J Vet Sci 2016, 19, 165–173, doi: 10.1515/pjvs-2016-0021.PMID:27096801.
Open DOI Search in Google Scholar Back to article
Mackness M.I., Harty D., Bhatnagar D., Winocour P.H., Arrol S., Ishola M., Durrington P.N.: Serum paraoxonase activity in familial hypercholesterolaemia and insulin-dependent diabetes mellitus. Atherosclerosis 1991, 86, 193–199, doi: 10.1016/0021-9150(91)90215-o.
Open DOI Search in Google Scholar Back to article
Mahboob M., Rahman M.F., Grover P.: Serum lipid peroxidation and antioxidant enzyme levels in male and female diabetic patients. Singapore Med J 2005, 46, 322–324.
Search in Google Scholar Back to article
Marco I.S., Lavin S.: Effect of the method of capture on the haematology and chemistry of red deer (Cervus elaphus). Res Vet Sci 1999, 66, 81–84, doi: 10.1053/rvsc.1998.0248.
Open DOI Search in Google Scholar Back to article
Monteith K.L., Stephenson T.R., Bleich V.C., Conner M.M., Pierce B.M., Bowyer R.T.: Risk-sensitive allocation in seasonal dynamics of fat and protein reserves in a long-lived mammal. J Anim Ecol 2013, 82, 377–388, doi: 10.1111/1365-2656.12016.
Open DOI Search in Google Scholar Back to article
Nie D., Gui J., Zhao N., Lin Y., Tang H., Cai F., Shen G., Liu J., Zhang E., Chen M.: Haematological and serum biochemical reference values in Chinese water deer (Hydropotes inermis): a preliminary study. BMC Vet Res 2020, 16, 395, doi: 10.1186/s12917-020-02601-2.
Open DOI Search in Google Scholar Back to article
Petrović M.Ž, Cincović M., Starič J., Djoković R., Belić B., Radinović M., Majkić M., Ilić Z.Ž.: The correlation between extracellular heat shock protein 70 and lipid metabolism in a ruminant model. Metabolites 2021, 12, 19, doi: 10.3390/metabo12010019.
Open DOI Search in Google Scholar Back to article
Pople N.C., Allen A.L, Woodbury M.R.: A retrospective study of neonatal mortality in farmed elk. Can Vet J 2001, 42, 925–928.
Search in Google Scholar Back to article
Rael L.T., Thomas G.W., Craun M.L., Curtis C.G., Bar-Or R., Bar-Or D.: Lipid peroxidation and the thiobarbituric acid assay: standardization of the assay when using saturated and unsaturated fatty acids. J Biochem Mol Biol 2004, 37, 749–752, doi: 10.5483/bmbrep.2004.37.6.749.
Open DOI Search in Google Scholar Back to article
Sueishi Y., Kamogawa E., Kimura A., Kitahara G., Satoh H., Asanuma T., Oowada S.: Multiple free-radical scavenging (MULTIS) capacity in cattle serum. J Clin Biochem Nutr 2017, 60, 76–80, doi: 10.3164/jcbn.15-142.
Open DOI Search in Google Scholar Back to article
Thorn C.E.: Section XIV, Normal Hematology of the Deer. In: Schalm’s Veterinary Hematology, 5th edition, edited by B.F. Feldman, J.G. Zinkl, N.C. Jain, Lippincott Williams & Wilkins, Philadelphia, PA, 2000, pp. 1179–1183.
Search in Google Scholar Back to article
Turk R., Juretic D., Geres D., Turk N., Rekić B., Simeon-Rudolf V., Svetina A.: Serum paraoxonase activity and lipid parameters in the early postpartum period of dairy cows. Res Vet Sci 2004, 76, 57–61, doi: 10.1016/j.rvsc.2003.08.001.
Open DOI Search in Google Scholar Back to article