Fig. 1
Scheme presenting the sequence of treatment of the uterine strips. ACh – acetylocholine; BPA – bisphenol A; BPF – bisphenol F. Concentrations of the applied substances are given in moles
Fig. 2
Representative diagrams showing contractile activity of the myometrial strips collected from immature pigs (A and D), cyclic pigs on days 12–14 of the oestrous cycle (B and E) and early pregnant pigs on days 12–16 of pregnancy (C and F) and treated with bisphenol A (BPA) and bisphenol F (BPF) at concentrations of 10−13 –10−1 mol/L
Fig. 3
Influence of bisphenol A on the tension (A), amplitude (B) and frequency (C) of contractions of the porcine uterine myometrial strips collected from immature pigs, cyclic pigs on days 12–14 of the oestrous cycle and early pregnant pigs on days 12–16 of pregnancy (n = 12 in each group). * – P < 0.05; ** – P < 0.01; *** – P < 0.001 compared to the contractile activity before the treatment
Fig. 4
Influence of bisphenol F on the tension (A), amplitude (B) and frequency (C) of contractions of the porcine uterine myometrial strips collected from immature pigs, cyclic pigs on days 12–14 of the estrous cycle and early pregnant pigs on days 12–16 of pregnancy (n = 12 in each group). *P < 0.05; **P < 0.01; ***P < 0.001 compared to the contractile activity before the treatment
Table 1
Dose-response parameters (arithmetic mean with 90% confidence interval), based on the tension, frequency and amplitude of contractions in immature pigs, cyclic pigs on days 12–14 of the estrous cycle and early pregnant pigs on days 12–16 of pregnancy treated with concentrations of bisphenol A and F increasing from 10−13 to 10−1 M
| Parameters | Tension | Amplitude | Frequency | ||||||
|---|---|---|---|---|---|---|---|---|---|
| Immature | Cyclic | Pregnant | Immature | Cyclic | Pregnant | Immature | Cyclic | Pregnant | |
| BPA 8.887 (8.433–9.341) | 6.451 (6.201–6.700) | 16.73 (15.64–17.81) | 24.82 (23.34–26.30) | 23.09 (22.21–23.97) | 27.20 (25.66–28.73) | 12.74 (11.17–14.31) | 6.037 (5.713–6.360) | 8.235 (6.842–9.629) | |
| Emax | 8.340 BPF (7.948–8.731) | 10.03 (9.776–10.29) | 8.386 (7.657–9.114) | 22.00 (21.45–22.56) | 28.77 (28.23–29.31) | 23.68 (21.24–26.13) | 9.702 (8.885–10.52) | 5.055 (4.808–5.302) | 4.978 (4.687–5.270) |
| 5.966 BPA (5.071–6.861) | 4.321 (2.913–5.728) | 10.09 (7.973–12.21) | 10.98 (8.672–13.28) | 12.11 (10.95–13.27) | 21.56 (21.01–22.11) | 4.188 (3.280–5.096) | 2.676 (2.122–3.229) | 4.325 (3.958–4.692) | |
| E0 | BPF 6.756 (6.561–6.950) | 8.619 (8.504–8.735) | 2.383 (0.1448–4.622) | −0.07270 (−0.4302–0.2848) | −1.726 (−2.557−0.8954) | 10.30 (8.076–12.53) | 0.1706 (−0.2099–0.5510) | 0.9818 (0.8362–1.127) | −0.7278 (−1.361−0.09438) |
| Span | BPA 2.921 (1.937–3.905) | 2.130 (0.7245–3.536) | 6.632 (4.296–8.968) | 13.84 (11.24–16.45) | 10.98 (9.603–12.36) | 5.635 (4.026–7.245) | 8.554 (6.768–10.34) | 3.361 (2.752–3.970) | 3.910 (2.478–5.342) |
| BPF 1.584 (1.153–2.015) | 1.415 (1.135–1.695) | 6.002 (3.812–8.192) | 22.08 (21.44–22.72) | 30.50 (29.55–31.44) | 13.38 (10.15–16.61) | 9.531 (8.639–10.42) | 4.073 (3.791–4.355) | 5.706 (5.052–6.360) | |
| −3.775 BPA (−4.633−2.916) | −1.758 (−2.769−0.7461) | −3.807 (−4.703−2.911) | −2.192 (−2.608−1.776) | −2.477 (−2.793−2.161) | −10.17 (−10.87−9.477) | −5.665 (−6.325−5.004) | −2.061 (−2.441−1.681) | −10.11 (−11.01−9.210) | |
| LogIC50 | −6.622 BPF (−7.489−5.755) | −7.314 (−7.925−6.703) | −1.634 (−2.302−0.9650) | −4.799 (−5.226−4.372) | −2.225 (−2.294−2.155) | −5.113 (−5.772−4.453) | −7.070 (−7.351−6.789) | −7.292 (−7.497−7.087) | −1.830 (−2.057−1.604) |
| BPA 1.554 | −11.34 | 24.19 | 9.012 | −5.332 | −11.53 | 1.141 | −30.13 | −18.68 | |
| AIC | BPF −37.77 | −50.61 | −8.395 | −19.02 | −17.05 | 14.35 | −19.84 | −46.59 | −32.40 |
| BPA 0.6702 | 0.4082 | 1.601 | 0.8928 | 0.5143 | 0.3188 | 0.6596 | 0.1981 | 0.3078 | |
| RMSE | BPF 0.1477 | 0.09013 | 0.4571 | 0.2268 | 0.3277 | 1.072 | 0.2943 | 0.08467 | 0.1816 |