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Preliminary study on gene regulation and its pathways in Chinese Holstein cows with clinical mastitis caused by Staphylococcus aureus Cover

Preliminary study on gene regulation and its pathways in Chinese Holstein cows with clinical mastitis caused by Staphylococcus aureus

Journal of Veterinary Research
Volume 66 (2022): Issue 2 (June 2022)
By: Wenjia Wang,  Rongling Li,  Tingzhu Ye,  Xinxin Zhang,  Chao Chen,  Ai-xin Liang and  Li-guo Yang  
Open Access
|May 2022

Figures & Tables

Fig. 1

Identification of Staphylococcus aureus isolated from milk samples from Holstein cows. A − specific PCR and electrophoresis map of the S. aureus protein A (spa) gene (323 bp); B − morphology of S. aureus colony on Baird-Parker agar
Identification of Staphylococcus aureus isolated from milk samples from Holstein cows. A − specific PCR and electrophoresis map of the S. aureus protein A (spa) gene (323 bp); B − morphology of S. aureus colony on Baird-Parker agar

Fig. 2

Transcriptome profiles of 16,544 genes identified by mRNA in peripheral blood leukocytes (PBL) from all the samples (A); volcano plot of global differentially expressed genes (DEGs) in PBL between the mastitic (M) and non-mastitic (NM) groups (B); and transcriptome profiles of 4,286 DEGs between the M and NM groups (C)
Transcriptome profiles of 16,544 genes identified by mRNA in peripheral blood leukocytes (PBL) from all the samples (A); volcano plot of global differentially expressed genes (DEGs) in PBL between the mastitic (M) and non-mastitic (NM) groups (B); and transcriptome profiles of 4,286 DEGs between the M and NM groups (C)

Fig. 3

The top 30 up- and downregulated differentially expressed genes analysed by Gene Ontology BP – biological processes; CC – cellular components; MF – molecular functions
The top 30 up- and downregulated differentially expressed genes analysed by Gene Ontology BP – biological processes; CC – cellular components; MF – molecular functions

Fig. 4

Pathways significantly enriched with the upregulated and downregulated DEGs, as observed by KEGG enrichment
Pathways significantly enriched with the upregulated and downregulated DEGs, as observed by KEGG enrichment

Fig. 5

DEGs downregulated in the metabolic pathway (A) and upregulated in platelet activation (B) observed by protein–protein interaction (PPI) analysis. The different colours represent degrees of connectivity
DEGs downregulated in the metabolic pathway (A) and upregulated in platelet activation (B) observed by protein–protein interaction (PPI) analysis. The different colours represent degrees of connectivity

Fig. 6

Concordance of gene expression profiles obtained by RNA-seq and qRT-PCR methods for four upregulated genes (VWF, ITGB3, F2RL3, and PRKG1) and three downregulated genes (GDA, HNMT, and GPT2). The β-actin gene was used as an internal reference control gene
Concordance of gene expression profiles obtained by RNA-seq and qRT-PCR methods for four upregulated genes (VWF, ITGB3, F2RL3, and PRKG1) and three downregulated genes (GDA, HNMT, and GPT2). The β-actin gene was used as an internal reference control gene

Characteristics of the seven experimental and control dairy cows

AnimalsGroupLactation (d)SCC (×104/mL)Milk (kg/day) yieldMilk morphologyBacterium
NM002NM2884.523normal−
NM003NM2565.119normal−
NM013NM2413.918normal−
NM182NM1984.420normal−
M008M2676235.0thin, clotsStaphylococcus aureus
M010M2134564.6flakesStaphylococcus aureus
M021M1865033.0clotsStaphylococcus aureus

RNA-seq data for the seven experimental and control dairy cows

SamplesTotal clean reads (bp)Clean reads Q20 (%)Clean reads Q30 (%)Total mapping ratio (%)
NM00247,493,26097.7891.0782.86
NM00347,472,82297.8391.3182.25
NM01347,646,18097.8791.3483.81
NM18247,588,37497.8491.2284.97
M00847,642,37297.9291.6479.93
M01047,710,37697.9691.8276.37
M02147,664,65297.9291.7577.52
DOI: https://doi.org/10.2478/jvetres-2022-0022 | Journal eISSN: 2450-8608
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Language: English
Page range: 179 - 187
Submitted on: Nov 23, 2021
Accepted on: Apr 21, 2022
Published on: May 5, 2022
Published by: National Veterinary Research Institute in Pulawy
In partnership with: Paradigm Publishing Services
Publication frequency: 4 issues per year
Keywords:
clinical mastitis,
cattle,
inflammatory response,
RNA-seq
Related subjects:
Life sciences,
Molecular biology,
Microbiology and virology,
Life sciences, other,
Medicine,
Veterinary medicine

© 2022 Wenjia Wang, Rongling Li, Tingzhu Ye, Xinxin Zhang, Chao Chen, Ai-xin Liang, Li-guo Yang, published by National Veterinary Research Institute in Pulawy
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

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