Table 1
The average number of cells from the granulocytic cell line per 1,000 porcine bone marrow cells (mean ± SD) before acrylamide administration
| Cell type | Control group | Low dose group | High dose group |
|---|---|---|---|
| Myeloblasts | 2.720 ± 0.504 | 2.980 ± 0.336 | 2.820 ± 0.344 |
| Promyelocytes | 2.080 ± 0.656 | 2.160 ± 0.672 | 2.240 ± 0.192 |
| Myelocyte | 3.600 ± 0.120 | 3.520 ± 0.144 | 3.200 ± 0.240 |
| Metamyelocyte | 6.500 ± 1.000 | 6.360 ± 0.568 | 6.360 ± 0.888 |
| Band neutrophils | 15.180 ± 2.584 | 13.620 ± 1.064 | 14.520 ± 1.576 |
| Neutrophilic granulocytes | 12.400 ± 2.200 | 13.380 ± 2.224 | 12.820 ± 2.296 |
| Eosinophilic myelocytes | 1.740 ± 0.416 | 1.320 ± 0.384 | 1.620 ± 0.264 |
| Eosinophilic metamyelocytes | 1.960 ± 0.552 | 2.260 ± 0.568 | 2.200 ± 0.280 |
| Band eosinophils | 2.640 ± 1.728 | 2.820 ± 1.624 | 2.780 ± 1.176 |
| Eosinophilic granulocytes | 1.300 ± 0.720 | 1.560 ± 1.272 | 1.960 ± 1.632 |
| Basophilic myelocytes | 0.000 | 0.000 | 0.000 |
| Basophilic metamyelocytes | 0.060 ± 0.048 | 0.020 ± 0.032 | 0.080 ± 0.064 |
| Band basophiles | 0.280 ± 0.136 | 0.320 ± 0.184 | 0.375 ± 0.225 |
| Basophilic granulocytes | 0.360 ± 0.192 | 0.280 ± 0.136 | 0.300 ± 0.160 |
| Hypersegmented granulocytes | 0.000 | 0.000 | 0.000 |
| Total granulocytes | 50.820 ± 1.184 | 50.600 ± 2.76 | 51.220 ± 0.896 |
Fig. 1
Representative cytological image of a bone marrow smear stained using the May–Grunwald–Giemsa method showing condensation and fragmentation of the nucleus of a neutrophil in the HD group. Magnification 1,000×
Fig. 2
Representative cytological image of a bone marrow smear stained using the May–Grunwald–Giemsa method showing condensation and fragmentation of the nucleus of a neutrophil in the HD group. Magnification 1,000×
Fig. 3
Representative cytological image of a bone marrow smear stained using the May–Grunwald–Giemsa method showing a basophilic myelocyte in the C group. Magnification 1,000×
Table 2
The average number of cells from the granulocytic cell line per 1,000 porcine bone marrow cells (mean ±SD) on the 28th day of the experiment
| Cell type | Control group | Low dose group | High dose group |
| Myeloblasts | 2.680 ± 0.576a, b | 1.160 ± 0.512a | 1.080 ± 0.104b |
| Promyelocytes | 2.100 ± 0.68a, b | 1.460 ± 0.728a | 1.320 ± 0.504b |
| Myelocyte | 3.540 ± 0.088a | 2.180 ± 0.896a, c | 3.300 ± 1.600c |
| Metamyelocyte | 6.540 ± 1.208a, b | 3.840 ± 1.568a, c | 3.120 ± 1.824b, c |
| Band neutrophils | 15.940 ± 2.872a, b | 8.620 ± 1.304a, c | 6.520 ± 1.944b, c |
| Neutrophilic granulocytes | 12.460 ± 2.192b | 10.920 ± 1.104c | 18.680 ± 9.016b, c |
| Eosinophilic myelocytes | 1.740 ± 0.456 | 1.180 ± 0.776 | 0.820 ± 0.384 |
| Eosinophilic metamyelocytes | 2.220 ± 0.464 | 2.320 ± 1.464 | 0.840 ± 0.568 |
| Band eosinophils | 2.960 ± 1.952b | 3.300 ± 2.200c | 1.420 ± 1.112b, c |
| Eosinophilic granulocytes | 2.000 ± 1.480a | 2.460 ± 1.632a, c | 2.120 ± 1.096a, c |
| Basophilic myelocytes | 0.020 ± 0.032 | 0.000 | 0.000 |
| Basophilic metamyelocytes | 0.080 ± 0.064 | 0.000 | 0.040 ± 0.048 |
| Band basophiles | 0.260 ± 0.192a, b | 0.060 ± 0.072a, c | 1.420 ± 1.112b, c |
| Basophilic granulocytes | 0.320 ± 0.184 a, b | 0.040 ± 0.064a, c | 0.080 ± 0.064b, c |
| Hypersegmented granulocytes | 0.000b | 0.000c | 0.500 ± 0.640b, c |
| Total granulocytes | 52.860 ± 2.528a, b | 37.520 ± 8.784a | 44.680 ± 3.144b |
Fig. 4
Representative cytological image of a bone marrow smear stained using the May–Grunwald–Giemsa method showing a myeloblast in the LD group. Magnification 1,000×