Acrylamide-induced changes of granulopoiesis in porcine bone marrow

Grzybowska, Dominika; Snarska, Anna

doi:10.2478/jvetres-2021-0040

Figures & Tables

Representative cytological image of a bone marrow smear stained using the May–Grunwald–Giemsa method showing condensation and fragmentation of the nucleus of a neutrophil in the HD group. Magnification 1,000×

Representative cytological image of a bone marrow smear stained using the May–Grunwald–Giemsa method showing a basophilic myelocyte in the C group. Magnification 1,000×

Representative cytological image of a bone marrow smear stained using the May–Grunwald–Giemsa method showing a myeloblast in the LD group. Magnification 1,000×

The average number of cells from the granulocytic cell line per 1,000 porcine bone marrow cells (mean ±SD) on the 28th day of the experiment

The average number of cells from the granulocytic cell line per 1,000 porcine bone marrow cells (mean ± SD) before acrylamide administration

Cell type	Control group	Low dose group	High dose group
Myeloblasts	2.720 ± 0.504	2.980 ± 0.336	2.820 ± 0.344
Promyelocytes	2.080 ± 0.656	2.160 ± 0.672	2.240 ± 0.192
Myelocyte	3.600 ± 0.120	3.520 ± 0.144	3.200 ± 0.240
Metamyelocyte	6.500 ± 1.000	6.360 ± 0.568	6.360 ± 0.888
Band neutrophils	15.180 ± 2.584	13.620 ± 1.064	14.520 ± 1.576
Neutrophilic granulocytes	12.400 ± 2.200	13.380 ± 2.224	12.820 ± 2.296
Eosinophilic myelocytes	1.740 ± 0.416	1.320 ± 0.384	1.620 ± 0.264
Eosinophilic metamyelocytes	1.960 ± 0.552	2.260 ± 0.568	2.200 ± 0.280
Band eosinophils	2.640 ± 1.728	2.820 ± 1.624	2.780 ± 1.176
Eosinophilic granulocytes	1.300 ± 0.720	1.560 ± 1.272	1.960 ± 1.632
Basophilic myelocytes	0.000	0.000	0.000
Basophilic metamyelocytes	0.060 ± 0.048	0.020 ± 0.032	0.080 ± 0.064
Band basophiles	0.280 ± 0.136	0.320 ± 0.184	0.375 ± 0.225
Basophilic granulocytes	0.360 ± 0.192	0.280 ± 0.136	0.300 ± 0.160
Hypersegmented granulocytes	0.000	0.000	0.000

Total granulocytes	50.820 ± 1.184	50.600 ± 2.76	51.220 ± 0.896

Acrylamide-induced changes of granulopoiesis in porcine bone marrow

Figures & Tables

Fig. 1

Fig. 2

Fig. 3

Fig. 4

The average number of cells from the granulocytic cell line per 1,000 porcine bone marrow cells (mean ±SD) on the 28th day of the experiment

The average number of cells from the granulocytic cell line per 1,000 porcine bone marrow cells (mean ± SD) before acrylamide administration

Paradigm

My account

Cell type	Control group	Low dose group	High dose group
Myeloblasts	2.680 ± 0.576 statistically significant difference between control and low dose group (P ≤ 0.05) , statistically significant difference between control and high dose group (P ≤ 0.05)	1.160 ± 0.512 statistically significant difference between control and low dose group (P ≤ 0.05)	1.080 ± 0.104 statistically significant difference between control and high dose group (P ≤ 0.05)
Promyelocytes	2.100 ± 0.68 statistically significant difference between control and low dose group (P ≤ 0.05) , statistically significant difference between control and high dose group (P ≤ 0.05)	1.460 ± 0.728 statistically significant difference between control and low dose group (P ≤ 0.05)	1.320 ± 0.504 statistically significant difference between control and high dose group (P ≤ 0.05)
Myelocyte	3.540 ± 0.088 statistically significant difference between control and low dose group (P ≤ 0.05)	2.180 ± 0.896 statistically significant difference between control and low dose group (P ≤ 0.05) , statistically significant difference between low dose and high dose group (P ≤ 0.05)	3.300 ± 1.600 statistically significant difference between low dose and high dose group (P ≤ 0.05)
Metamyelocyte	6.540 ± 1.208 statistically significant difference between control and low dose group (P ≤ 0.05) , statistically significant difference between control and high dose group (P ≤ 0.05)	3.840 ± 1.568 statistically significant difference between control and low dose group (P ≤ 0.05) , statistically significant difference between low dose and high dose group (P ≤ 0.05)	3.120 ± 1.824 statistically significant difference between control and high dose group (P ≤ 0.05) , statistically significant difference between low dose and high dose group (P ≤ 0.05)
Band neutrophils	15.940 ± 2.872 statistically significant difference between control and low dose group (P ≤ 0.05) , statistically significant difference between control and high dose group (P ≤ 0.05)	8.620 ± 1.304 statistically significant difference between control and low dose group (P ≤ 0.05) , statistically significant difference between low dose and high dose group (P ≤ 0.05)	6.520 ± 1.944 statistically significant difference between control and high dose group (P ≤ 0.05) , statistically significant difference between low dose and high dose group (P ≤ 0.05)
Neutrophilic granulocytes	12.460 ± 2.192 statistically significant difference between control and high dose group (P ≤ 0.05)	10.920 ± 1.104 statistically significant difference between low dose and high dose group (P ≤ 0.05)	18.680 ± 9.016 statistically significant difference between control and high dose group (P ≤ 0.05) , statistically significant difference between low dose and high dose group (P ≤ 0.05)
Eosinophilic myelocytes	1.740 ± 0.456	1.180 ± 0.776	0.820 ± 0.384
Eosinophilic metamyelocytes	2.220 ± 0.464	2.320 ± 1.464	0.840 ± 0.568
Band eosinophils	2.960 ± 1.952 statistically significant difference between control and high dose group (P ≤ 0.05)	3.300 ± 2.200 statistically significant difference between low dose and high dose group (P ≤ 0.05)	1.420 ± 1.112 statistically significant difference between control and high dose group (P ≤ 0.05) , statistically significant difference between low dose and high dose group (P ≤ 0.05)
Eosinophilic granulocytes	2.000 ± 1.480 statistically significant difference between control and low dose group (P ≤ 0.05)	2.460 ± 1.632 statistically significant difference between control and low dose group (P ≤ 0.05) , statistically significant difference between low dose and high dose group (P ≤ 0.05)	2.120 ± 1.096 statistically significant difference between control and low dose group (P ≤ 0.05) , statistically significant difference between low dose and high dose group (P ≤ 0.05)
Basophilic myelocytes	0.020 ± 0.032	0.000	0.000
Basophilic metamyelocytes	0.080 ± 0.064	0.000	0.040 ± 0.048
Band basophiles	0.260 ± 0.192 statistically significant difference between control and low dose group (P ≤ 0.05) , statistically significant difference between control and high dose group (P ≤ 0.05)	0.060 ± 0.072 statistically significant difference between control and low dose group (P ≤ 0.05) , statistically significant difference between low dose and high dose group (P ≤ 0.05)	1.420 ± 1.112 statistically significant difference between control and high dose group (P ≤ 0.05) , statistically significant difference between low dose and high dose group (P ≤ 0.05)
Basophilic granulocytes	0.320 ± 0.184 statistically significant difference between control and low dose group (P ≤ 0.05) , statistically significant difference between control and high dose group (P ≤ 0.05)	0.040 ± 0.064 statistically significant difference between control and low dose group (P ≤ 0.05) , statistically significant difference between low dose and high dose group (P ≤ 0.05)	0.080 ± 0.064 statistically significant difference between control and high dose group (P ≤ 0.05) , statistically significant difference between low dose and high dose group (P ≤ 0.05)
Hypersegmented granulocytes	0.000 statistically significant difference between control and high dose group (P ≤ 0.05)	0.000 statistically significant difference between low dose and high dose group (P ≤ 0.05)	0.500 ± 0.640 statistically significant difference between control and high dose group (P ≤ 0.05) , statistically significant difference between low dose and high dose group (P ≤ 0.05)

Total granulocytes	52.860 ± 2.528 statistically significant difference between control and low dose group (P ≤ 0.05) , statistically significant difference between control and high dose group (P ≤ 0.05)	37.520 ± 8.784 statistically significant difference between control and low dose group (P ≤ 0.05)	44.680 ± 3.144 statistically significant difference between control and high dose group (P ≤ 0.05)