Fig. 1
HPLC-DAD chromatogram of AMO standard solution
Fig. 2
HPLC chromatogram of blank pig feed
Fig. 3
HPLC chromatogram of blank pig feed spiked with AMO at a concentration of 150 mg/kg
Table 1
Validation parameters of optimised HPLC-DAD method
| Validation parameters | Results |
|---|---|
| Solvent STD regression equations | y = 80.695x + 392.76 |
| Coefficient of determination (R2) | 0.99 |
| Recovery (%) | |
| 150 mg/kg | 76.1 |
| 300 mg/kg | 78.2 |
| 500 mg/kg | 81.6 |
| Repeatability (%) | |
| 150 mg/kg | 7.2 |
| 300 mg/kg | 5.8 |
| 500 mg/kg | 3.7 |
| Within-laboratory reproducibility (%) | |
| 150 mg/kg | 7.6 |
| 300 mg/kg | 6.8 |
| 500 mg/kg | 5.3 |
| LOD (mg/kg) | 51.2 |
| LOQ (mg/kg) | 103.0 |
| U (%) | 19.4 |