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Field trial of primary and booster dose of inactivated vaccine against bovine respiratory bacteria in young Holstein calves Cover

Field trial of primary and booster dose of inactivated vaccine against bovine respiratory bacteria in young Holstein calves

Journal of Veterinary Research
Volume 64 (2020): Issue 2 (June 2020)
By: Kazusa Mori,  Toshihide Kato,  Osamu Yokota and  Hiromichi Ohtsuka  
Open Access
|Jun 2020

Figures & Tables

Fig. 1

Antibody response to the vaccinationsAntibody titres against H. somni (Hs), P. multocida (Pm), and M. haemolytica (Mh) in (1) the first experiment and (2) the second experiment. Calves received (1) the primary vaccination at the age of 5–12 days and the booster dose at 2, 3 or 4 weeks later or (2) the primary vaccination at 1, 2 or 3 weeks of age and the booster dose at 4 weeks later. (1) In control group calves, blood was drawn at the age of 5–12 days and calves received the vaccination only once 2 weeks later (between 19 and 26 days of age). A, B – P < 0.01; a, b – P < 0.05; arrows – time of vaccination
Antibody response to the vaccinationsAntibody titres against H. somni (Hs), P. multocida (Pm), and M. haemolytica (Mh) in (1) the first experiment and (2) the second experiment. Calves received (1) the primary vaccination at the age of 5–12 days and the booster dose at 2, 3 or 4 weeks later or (2) the primary vaccination at 1, 2 or 3 weeks of age and the booster dose at 4 weeks later. (1) In control group calves, blood was drawn at the age of 5–12 days and calves received the vaccination only once 2 weeks later (between 19 and 26 days of age). A, B – P < 0.01; a, b – P < 0.05; arrows – time of vaccination

Fig. 2

Age at the primary vaccinationCumulative distribution of age in days at the time of the primary vaccination in the first experiment; a–d – comparison between M. haemolytica (Mh) positive and negative; e – comparison between H. somni (Hs) positive and negative; ** – P < 0.01; # – P = 0.11
Age at the primary vaccinationCumulative distribution of age in days at the time of the primary vaccination in the first experiment; a–d – comparison between M. haemolytica (Mh) positive and negative; e – comparison between H. somni (Hs) positive and negative; ** – P < 0.01; # – P = 0.11

Fig. 3

Antibody response to the vaccination among calves of different ages at the primary dose Antibody titres against H. somni (Hs), P. multocida (Pm), and M. haemolytica (Mh) of calves which received the primary vaccination at the age of 7–12 days (7–12 day group) and 5 or 6 days (5–6 day group). Calves were also vaccinated 2, 3, or 4 weeks later. A, B, C – P < 0.01; a, b – P < 0.05; # – P = 0.051; α, β – 0.073; arrows – time of vaccination
Antibody response to the vaccination among calves of different ages at the primary dose Antibody titres against H. somni (Hs), P. multocida (Pm), and M. haemolytica (Mh) of calves which received the primary vaccination at the age of 7–12 days (7–12 day group) and 5 or 6 days (5–6 day group). Calves were also vaccinated 2, 3, or 4 weeks later. A, B, C – P < 0.01; a, b – P < 0.05; # – P = 0.051; α, β – 0.073; arrows – time of vaccination

Fig. 4

Antibody response to the vaccination among calves with different maternal antibody levels (MAL) Antibody titres against M. haemolytica (Mh) and P. multocida (Pm) in (1) the first experiment and in (2) the second experiment for the high, low, and control groups. A, B P < 0.01; a, b P < 0.05; # P = 0.18; arrows – time of vaccination
Antibody response to the vaccination among calves with different maternal antibody levels (MAL) Antibody titres against M. haemolytica (Mh) and P. multocida (Pm) in (1) the first experiment and in (2) the second experiment for the high, low, and control groups. A, B P < 0.01; a, b P < 0.05; # P = 0.18; arrows – time of vaccination

Time schedules of vaccination and blood sampling

First experiment
       Weeks after primary vaccination (or first blood sampling)01234567Sample size
       +2 groupPrimary*BoosterBSn = 21
       +3 groupPrimary*BoosterBSn = 16
       +4 groupPrimary*BoosterBSn = 19
       Control groupBS**VaccinationBSn = 15

j_jvetres-2020-0042_tab_005_w2aab3b7c90b1b6b1ab1b1b8Aa

Maternal antibody levelPrimary vaccinationBooster vaccinationBlood samplingSample size
       Mh high groupMh: 200 or overn = 11
       Mh low groupMh: under 200the age of 1–3 weeks4 weeks3 weeksn = 20
       Pm high groupPm: 100 or overafter primary vaccinationafter booster vaccinationn = 18
       Pm low groupPm: under 100n = 13

j_jvetres-2020-0042_tab_004_w2aab3b7c90b1b6b1ab1b1b7Aa

Second experiment
       Age (weeks)123456789101112Sample size
       1+4 GroupPrimaryBoosterBSBSn = 10
       2+4 GroupPrimaryBoosterBSBSn = 11
       3+4 GroupPrimaryBoosterBSBSn = 10

Number and percentage of seropositive or seronegative calves at 3 weeks after the booster dose

First ExperimentSecond Experiment
+2+3+4Cont.7–125–6HighLow1+42+43+4HighLow
H. somniPositive19141933616--10108--
90.5%87.5%100%20.0%90.0%100%--100%91.0%80.0%--
Negative2201240--012--
9.5%12.5%0.0%80.0%10.0%0.0%--0.0%9.0%20.0%--
P. multocidaPositive2016199391625141011101813
92.5%100%100%60.0%97.5%100%100%93.3%100%100%100%100%100%
Negative1006100100000
4.8%0.0%0.0%40.0%2.5%0.0%0.0%6.7%0.0%0.0%0.0%0.0%0.0%
M. haemolyticaPositive1110120294181162154
52.4%62.5%63.2%0.0%72.5%25.0%85.7%57.9%60.0%18.0%10.0%45.5%20.0%
Negative106715111238499616
47.6%37.5%36.8%100.0%27.5%75.0%14.3%42.1%40.0%82.0%90.0%54.5%80.0%

j_jvetres-2020-0042_tab_003_w2aab3b7c90b1b6b1ab1b1b6Aa

Maternal antibody levelPrimary vaccinationBooster vaccinationBlood samplingSample size
       Mh High groupMh: 200 or overn = 21
       Mh Low groupMh: under 2002, 3 or 4 weeks3 weeksn = 19
       Pm High groupPm: 100 or overthe age of 7–12 daysafter primary vaccinationafter booster vaccinationn = 25
       Pm Low groupPm: under 100n = 15

j_jvetres-2020-0042_tab_002_w2aab3b7c90b1b6b1ab1b1b5Aa

Primary vaccinationBooster vaccinationBlood samplingSample size
       7–12 day groupage of 7–12 days2, 3 or 4 weeks after3 weeks after boostern = 40
       5–6 day groupage of 5 or 6 daysprimary vaccinationvaccinationn = 16
DOI: https://doi.org/10.2478/jvetres-2020-0042 | Journal eISSN: 2450-8608
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Language: English
Page range: 223 - 230
Submitted on: Sep 26, 2019
Accepted on: Jun 1, 2020
Published on: Jun 16, 2020
Published by: National Veterinary Research Institute in Pulawy
In partnership with: Paradigm Publishing Services
Publication frequency: 4 issues per year
Keywords:
bovine respiratory disease,
young calves,
early vaccination
Related subjects:
Life sciences,
Molecular biology,
Microbiology and virology,
Life sciences, other,
Medicine,
Veterinary medicine

© 2020 Kazusa Mori, Toshihide Kato, Osamu Yokota, Hiromichi Ohtsuka, published by National Veterinary Research Institute in Pulawy
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

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