Table 1
Expression of tlr 2 at the mRNA level in the intestines of uninfected and T. spiralis-infected mice determined using qPCR
| Days post infection (dpi) | Expression of mRNA tlr2 in the jejunum | Expression of mRNA tlr2 in the colon |
|---|---|---|
| 0 (uninfected) | 0.7 ± 0.19 | 0.6 ± 0.16 |
| 4 | 1.5 ± 0.24* | 0.7 ± 0.04 |
| 8 | 1.4 ± 0.26* | 0.6 ± 0.07 |
| 16 | 1.4 ± 0.23* | 0.6 ± 0.10 |
Fig. 1
Representative microphotography showing expression of TLR2 at the protein level in intestines isolated from control (uninfected) and T. spiralis-infected mice
A – jejunum of T. spiralis-infected mouse at 4 dpi with many brown-stained, TLR2-positive epithelial cells (20×);
B – jejunum of T. spiralis-infected mouse at 8 dpi with similar, strong immunohistochemical positive reaction indicating presence of TLR2 protein in epithelial cells (40×);
C – jejunum from control (uninfected) mouse with almost no positive reaction for TLR2 protein (10×);
D – colon from uninfected mouse also with almost no positive reaction for TLR2 (20x)
Fig 2
Trends of changes in mRNA expression of the tlr2 gene in the intestinal phase of T. spiralis infection