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Changes in the expression of TLR2 during the intestinal phase of trichinellosis Cover

Changes in the expression of TLR2 during the intestinal phase of trichinellosis

Journal of Veterinary Research
Volume 64 (2020): Issue 2 (June 2020)
By: Agnieszka Wojtkowiak-Giera,  Monika Derda,  Elżbieta Wandurska-Nowak,  Paweł P. Jagodziński,  Agnieszka Kolasa-Wołosiuk,  Danuta Kosik-Bogacka and  Edward Hadaś  
Open Access
|Apr 2020

Figures & Tables

Fig. 1

Representative microphotography showing expression of TLR2 at the protein level in intestines isolated from control (uninfected) and T. spiralis-infected miceA – jejunum of T. spiralis-infected mouse at 4 dpi with many brown-stained, TLR2-positive epithelial cells (20×);B – jejunum of T. spiralis-infected mouse at 8 dpi with similar, strong immunohistochemical positive reaction indicating presence of TLR2 protein in epithelial cells (40×);C – jejunum from control (uninfected) mouse with almost no positive reaction for TLR2 protein (10×);D – colon from uninfected mouse also with almost no positive reaction for TLR2 (20x)
Representative microphotography showing expression of TLR2 at the protein level in intestines isolated from control (uninfected) and T. spiralis-infected miceA – jejunum of T. spiralis-infected mouse at 4 dpi with many brown-stained, TLR2-positive epithelial cells (20×);B – jejunum of T. spiralis-infected mouse at 8 dpi with similar, strong immunohistochemical positive reaction indicating presence of TLR2 protein in epithelial cells (40×);C – jejunum from control (uninfected) mouse with almost no positive reaction for TLR2 protein (10×);D – colon from uninfected mouse also with almost no positive reaction for TLR2 (20x)

Fig 2

Trends of changes in mRNA expression of the tlr2 gene in the intestinal phase of T. spiralis infection
Trends of changes in mRNA expression of the tlr2 gene in the intestinal phase of T. spiralis infection

Expression of tlr 2 at the mRNA level in the intestines of uninfected and T_ spiralis-infected mice determined using qPCR

Days post infection (dpi)Expression of mRNA tlr2 in the jejunumExpression of mRNA tlr2 in the colon
0 (uninfected)0.7 ± 0.190.6 ± 0.16
41.5 ± 0.24*0.7 ± 0.04
81.4 ± 0.26*0.6 ± 0.07
161.4 ± 0.23*0.6 ± 0.10
DOI: https://doi.org/10.2478/jvetres-2020-0027 | Journal eISSN: 2450-8608
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Language: English
Page range: 269 - 274
Submitted on: May 22, 2019
Accepted on: Apr 3, 2020
Published on: Apr 21, 2020
Published by: National Veterinary Research Institute in Pulawy
In partnership with: Paradigm Publishing Services
Publication frequency: 4 issues per year
Keywords:
mice,
toll-like receptor 2,
quantitative real-time PCR,
immunohistochemical staining
Related subjects:
Life sciences,
Molecular biology,
Microbiology and virology,
Life sciences, other,
Medicine,
Veterinary medicine

© 2020 Agnieszka Wojtkowiak-Giera, Monika Derda, Elżbieta Wandurska-Nowak, Paweł P. Jagodziński, Agnieszka Kolasa-Wołosiuk, Danuta Kosik-Bogacka, Edward Hadaś, published by National Veterinary Research Institute in Pulawy
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

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