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Metagenomic analysis of acquired antibiotic resistance determinants in the gut microbiota of wild boars (Sus scrofa) – preliminary results Cover

Metagenomic analysis of acquired antibiotic resistance determinants in the gut microbiota of wild boars (Sus scrofa) – preliminary results

Journal of Veterinary Research
Volume 64 (2020): Issue 1 (March 2020)
By: Balázs Libisch,  Tibor Keresztény,  Zoltán Kerényi,  Róbert Kocsis,  Rita Sipos,  Péter P. Papp and  Ferenc Olasz  
Open Access
|Feb 2020

Figures & Tables

Fig. 1

Phylum-level bacterial composition of the microbiota in three sections of the gastrointestinal tract of the diarrhoeic wild boar (WB) from Hungary analysed in this work in comparison with those established earlier for the domestic pig (DP) by a meta-analysis (13)

Fig. 2

GrapeTree diagram generated in EnteroBase based on seven-gene MLST profiles of 104 E. coli strains isolated in Europe from wild boars and domestic pigs (2, 28, 29). Countries of origin (and number of strains): Denmark (48), Hungary (8), Czech Republic (7), Spain (7), Ireland (6), Luxembourg (5), Italy (4), Switzerland (3), United Kingdom (3), Croatia (2), France (2), Germany (2), Portugal (2), Austria (1), Belgium (1), Bulgaria (1), Netherlands (1), and Sweden (1). Dark blue, light blue, and orange indicate E. coli isolates of domestic pig, wild boar, and food origin, respectively. Numbers stand for sequence types by the Achtman seven-gene MLST scheme (28). The node size in the GrapeTree diagram is proportional to the number of bacterial strains with the same sequence type (ST), and the length of edges are proportional to inter-node genetic distances (29). ST388 determined for the E. coli O112ab:H2 strain characterised in this work is marked by an asterisk (*)

Fig. 3

Immediate genetic environment of the tetracycline resistance determinants identified from the gut microbiome of free-living wild boars in Hungary. Schematic diagrams of metagenomic contigs for the tetQ (A), tetW (B), and tetO genes (C), respectively. Abbreviations for selected genes: traG – conjugal transfer protein TraG; tnp – transposase; mobC – mobilisation protein MobC; tnpV – transposon-encoded protein TnpV; and ctp – conjugal transfer protein

Fig. 4

Proportions of the sales of various veterinary antimicrobial agents in three countries of the European Union in 2015 (9) compared to those in Japan in 2013 for food producing animals (4)

Examples of acquired resistance determinants against various antibiotic classes in wastewater metagenomes in Hungary

LocationTetracyclinesMacrolidesSulphonamidesβ-lactamsAminoglycosidesNCBI SRA Codes
North PesttetW, tetO, tetMmefA, mphE, ermB, ermF, ermGsul1blaOXAaadA11, aadA1, aph(6)-IdERR2607463
North PesttetQ, tetW, tetO, tetMmefA, mphE, ermB, ermF, ermGsul1, sul2cfxA , blaOXA, blaTEMaadA11, aph(6)-IdERR1713361
DOI: https://doi.org/10.2478/jvetres-2020-0015 | Journal eISSN: 2450-8608
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Language: English
Page range: 111 - 118
Submitted on: Jun 3, 2019
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Accepted on: Feb 13, 2020
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Published on: Feb 29, 2020
Published by: National Veterinary Research Institute in Pulawy
In partnership with: Paradigm Publishing Services
Publication frequency: 4 issues per year
Keywords:
wild boar,
intestinal microbiota,
antibiotic resistance,
tetracycline,
natural habitat
Related subjects:
Life sciences,
Molecular biology,
Microbiology and virology,
Life sciences, other,
Medicine,
Veterinary medicine

© 2020 Balázs Libisch, Tibor Keresztény, Zoltán Kerényi, Róbert Kocsis, Rita Sipos, Péter P. Papp, Ferenc Olasz, published by National Veterinary Research Institute in Pulawy
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

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