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Molecular detection and genetic diversity of porcine circovirus type 3 in commercial pig farms in Xinjiang province, China Cover

Molecular detection and genetic diversity of porcine circovirus type 3 in commercial pig farms in Xinjiang province, China

Journal of Veterinary Research
Volume 63 (2019): Issue 4 (December 2019)
By: Qiao Mengfan,  Wang Xifeng,  Zhang Guowu,  Meng Qingling,  Qiao Jun,  Wang Lixia,  Cai Kuojun,  Zhang Jinsheng,  Zhang Zaichao,  Yu Weiwei,  Peng Yelong and  Cai Xuepeng  
Open Access
|Dec 2019

Figures & Tables

Fig. 1

A – geographic distribution of sampled farms, B – positive rates of PCV3 infection in different commercial pig farms in Xinjiang province, China Note: Positive rate was determined based on PCR analysis of clinical samples.
A – geographic distribution of sampled farms, B – positive rates of PCV3 infection in different commercial pig farms in Xinjiang province, China Note: Positive rate was determined based on PCR analysis of clinical samples.

Fig. 2

Phylogenetic analysis of PCV3 strains based on the full-length cap gene using the neighbour-joining method Note: The nucleotide sequences of cap gene of PCV3 strains obtained in this study and available in GenBank were used to construct a phylogenetic tree by the neighbour-joining method. Bootstrap values were calculated with 1,000 replicates. Vertical lines are used to indicate groups (or subgroups) which were referred to in the text. Filled circles indicate the PCV3 strains identified in this study
Phylogenetic analysis of PCV3 strains based on the full-length cap gene using the neighbour-joining method Note: The nucleotide sequences of cap gene of PCV3 strains obtained in this study and available in GenBank were used to construct a phylogenetic tree by the neighbour-joining method. Bootstrap values were calculated with 1,000 replicates. Vertical lines are used to indicate groups (or subgroups) which were referred to in the text. Filled circles indicate the PCV3 strains identified in this study

Genetic diversity of PCV3 strains circulating in commercial pig farms in Xinjiang province, China

PCV3 strainsGroupSubgroup
CN/Xinjiang-SH6/201811.1
CN/Xinjiang-TA36/201811.1
CN/Xinjiang-AL5/201811.2
CN/Xinjiang-UR22/201811.1
CN/Xinjiang-YI7/201822.1
CN/Xinjiang-CH29/201822.2
CN/Xinjiang-AK16/201822.1
CN/Xinjiang-KO17/201822.2
CN/Xinjiang-KA2/201811.1

List of primer sequences used in this study

Primer nameNucleotide sequencePosition in referenceProduct size
(5′→ 3′)sequence(bp)
FP1CCGTAGAAGTCTGTCATTCCAG1383–1404
RP1AAGCCCTGGCACGCCAACCAC1796–1816434
FP2TTAGAGAACGGACTTGTAACGA1343–1364
RP2ATGAGACACAGAGCTATATTCAG1965–1987645

GenBank accession numbers of PCV3 strains

StrainAccession number
2164KX458235
PCV3-US/MN2016KX898030
PCV3-US/SD2016KX966193
PCV3/CN/Fujian-5/2016KY075986
PCV3/CN/Henan-13/2016KY075988
PCV3/CN/Jiangxi-62/2016KY075989
PCV3/CN/Chongqing-150/2016KY075992
CN/Hubei-618/2016KY354039
CCV-AKY363870
CCV-BKY363871
CCV-CKY363872
PCV3-China/GD2016KY418606
PCV3/KU-1601KY996337
PCV3/KU-1604KY996340
PCV3/KU-1606KY996342
PCV3/KU-1608KY996344
PCV3/KU-1609KY996345
P1705SCYC/2017MF063070
16R927/2016MF063071
PCV3-BR/RS/6MF079253
PCV3/CN/Jiangxi-B1/2017MF589107
PCV3/CN/Jiangxi-S1/2017MF589133
PCV3/CN/Guangdong-CH/2016MF589112
PCV3/CN/Guangdong-X1/2016MF589118
309MF589652
JX-1/CH/2017MF677838
1621_Italy_2017MF805719
4332-5_Denmark_2017MF805723
4332-7_Denmark_2017MF805724
DE2.8MG014377
DE19.15MG014367
DE27.16MG014370
PCV3/HU/Szerencs/2017MG595741
PCV3-RU/TY17MG679916
PCV3-JSXY-201701MG868940
PCV3-HBWH-201703MG868941
PCV3-SH-201705MG868945
SDMG947596
PCV3-CN2018HLG-5MH277111
PCV3-CN2018JL-1MH277112
PCV3-CN2018LN-3MH277117
COL/Cundinamarca2/2018MH327785
CN/Xinjiang-AK16/2018MK562412
CN/Xinjiang-AL5/2018MK562413
CN/Xinjiang-CH29/2018MK562414
CN/Xinjiang-KA2/2018MK562415
CN/Xinjiang-KO17/2018MK562416
CN/Xinjiang-SH6/2018MK562417
CN/Xinjiang-TA36/2018MK562418
CN/Xinjiang-UR22/2018MK562419
CN/Xinjiang-YI7/2018MK562420
29160NC031753

Detection of PCV3 infection in different samples from commercial pig farms in Xinjiang province, China

Clinical samplesNumber of samplesNumber of positive samplesPositive rate (%) of PCV3
Lymph nodes792936.71 (29/79) a
Spleen932223.66 (22/93) a
Lung62914.52 (9/62) b
Pleural effusion571322.81 (13/57) a
Serum1021514.71 (15/102) b
Total3938822.39 (88/393)
DOI: https://doi.org/10.2478/jvetres-2019-0071 | Journal eISSN: 2450-8608
Journal RSS Feed
Language: English
Page range: 481 - 488
Submitted on: May 7, 2019
|
Accepted on: Nov 22, 2019
|
Published on: Dec 19, 2019
Published by: National Veterinary Research Institute in Pulawy
In partnership with: Paradigm Publishing Services
Publication frequency: 4 issues per year
Keywords:
type 3,
molecular detection,
genetic diversity,
gene,
China
Related subjects:
Life sciences,
Molecular biology,
Microbiology and virology,
Life sciences, other,
Medicine,
Veterinary medicine

© 2019 Qiao Mengfan, Wang Xifeng, Zhang Guowu, Meng Qingling, Qiao Jun, Wang Lixia, Cai Kuojun, Zhang Jinsheng, Zhang Zaichao, Yu Weiwei, Peng Yelong, Cai Xuepeng, published by National Veterinary Research Institute in Pulawy
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

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