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GLP-1 localisation and proglucagon gene expression in healthy and diabetic mouse ileum

Journal of Veterinary Research
Volume 62 (2018): Issue 2 (June 2018)
By:
Open Access
|Jul 2018

Figures & Tables

Fig. 1

General view of ileum tissue. Diabetic group. Triple staining. Bar: 100 μm
General view of ileum tissue. Diabetic group. Triple staining. Bar: 100 μm

Fig. 2

GLP-1 immunoreactivity. Diabetic group. Arrows show GLP-1 immunoreactivity. Bar: 50 μm
GLP-1 immunoreactivity. Diabetic group. Arrows show GLP-1 immunoreactivity. Bar: 50 μm

Fig. 3

GLP-1 immunoreactivity. Diabetic group. Arrow shows GLP-1 immunoreactivity. Bar: 20 μm
GLP-1 immunoreactivity. Diabetic group. Arrow shows GLP-1 immunoreactivity. Bar: 20 μm

Fig. 4

GLP-1 immunoreactivity. Sham group. Bar: 50 μm
GLP-1 immunoreactivity. Sham group. Bar: 50 μm

Fig. 5

GLP-1 Immunoreactivity in diabetic, control, and sham groups. Arrows show GLP-1 immunoreactivity. Bars: 20 μm
GLP-1 Immunoreactivity in diabetic, control, and sham groups. Arrows show GLP-1 immunoreactivity. Bars: 20 μm

Fig. 6

Negative control group. Bar: 100 μm
Negative control group. Bar: 100 μm

Fig. 7

RT PCR results for proglucagon and β-actin genes. M – 100 bp DNA ladder, 1–6 – diabetic group, 7–12 – control group, 12– 18 – sham group
RT PCR results for proglucagon and β-actin genes. M – 100 bp DNA ladder, 1–6 – diabetic group, 7–12 – control group, 12– 18 – sham group

Fig. 8

Comparison of mean ±standard deviation among groups in terms of proglucagon gene expression. P > 0.05
Comparison of mean ±standard deviation among groups in terms of proglucagon gene expression. P > 0.05

Blood glucose level (mg/dL) on the 3rd day_ Comparison among groups

GroupNBlood glucose (mg/dL) minimumBlood glucose (mg/dL) maximumBlood glucose (mg/dL) mean ± SD
Diabetic6240360275.66a ± 45.71
Control6748379.50b ± 3.27
Sham6728479.33b ± 4.13

Sequences of β-actin and proglucagon (GCG) specific primer pairs

GenePrimers of genesReference number
β-actin 5'5'-TCATGAAGTGTGACGTTGACATCCGT-3'20
β-actin 3'5'-CCTAGAAGCATTTGCGGTGCACGATG-3'20
Proglucagon(GCG) 5'5'-GACTTCCCAGAAGAAGTCGCCAT-3'28
Proglucagon(GCG) 3'5'-CTACGGTTACCAGGTGGTCATGT-3'28
DOI: https://doi.org/10.2478/jvetres-2018-0033 | Journal eISSN: 2450-8608
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Language: English
Page range: 237 - 242
Submitted on: Mar 29, 2018
Accepted on: Jun 22, 2018
Published on: Jul 7, 2018
Published by: National Veterinary Research Institute in Pulawy
In partnership with: Paradigm Publishing Services
Publication frequency: 4 issues per year
Keywords:
mice,
diabetes mellitus,
GLP-1,
L cells,
proglucagon
Related subjects:
Life sciences,
Molecular biology,
Microbiology and virology,
Life sciences, other,
Medicine,
Veterinary medicine

© 2018 Serap Koral Taşçı, Seyit Ali Bingöl, published by National Veterinary Research Institute in Pulawy
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

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