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The Effect of Trifluralin on Post-in vitro Morphogenesis of Five Genotypes of Head Cabbage (Brassica oleraceae var. capitata) Cover

The Effect of Trifluralin on Post-in vitro Morphogenesis of Five Genotypes of Head Cabbage (Brassica oleraceae var. capitata)

Journal of Horticultural Research
Volume 29 (2021): Issue 1 (June 2021)
By: ,   and    
Open Access
|May 2021
Figures & tables

Figures & Tables

Figure 1

Organogenesis of cabbage in in vitro cultures: A – shoot induction from cut ends of treated hypocotyl on shoot induction medium in control (bar = 0.5 cm); B – shoot induction from trifluralin treated callus on shoot induction medium (bar = 0.5 cm); C – green dormant callus from treated calli on shoot induction medium (bar = 0.5 cm); D – germinating seeds treated with trifluralin after seven days on seed germination medium (bar = 1 cm); E – growing calli from treated hypocotyls after 14 days on shoot induction medium (bar = 0.5 cm); F – rooted shootlets derived from treated seeds

Table 1

Results of ANOVA to test the influence of cultivar, trifluralin concentration, treatment condition on in vitro culture of cabbage starting from seeds

TraitEffectMSFP
Rate of seed germinationcultivar11339.387.40.0000*
trifluralin concentration33654.1259.30.0000*
treatment condition7613.458.6680.0000*
treatment condition × cultivar638.64.92130.0000*
treatment condition × concentration382.42.94650.0015*
concentration × cultivar925.47.1310.000*
treatment × concentration × cultivar237.41.8290.00262*
Shoots per germinated seedcultivar17.912.8130.014*
trifluralin concentration4.40.6920.5990
treatment condition6.51.0210.3867
treatment condition × cultivar10.051.57950.0799
treatment condition × concentration9.341.46740.1492
concentration × cultivar9.531.49720.0860
treatment × concentration × cultivar6.961.0940.337
Rate of root inductioncultivar10575.347.10.0000*
trifluralin concentration23284.8103.60.0000*
treatment condition6557.229.1860.0000*
treatment condition × cultivar377.31.67930.05559
treatment condition × concentration717.03.19120.00068*
concentration × cultivar1636.57.28420.0000*
treatment × concentration × cultivar446.71.9880.000749*
Figure 2

Effect of cultivar, trifluralin concentration, treatment condition on in vitro culture of seeds and their interaction with each other: A1, B1, C1 – the effect of different treatment conditions on the rate of seed germination, shoot induction, and root induction respectively; A2, B2, C2 – the effect of different cultivars on the rate of seed germination, shoot induction, and root induction respectively; A3, B3, C3 – the effect of different concentrations of trifluralin on seed germination, shoot induction, and root induction respectively. Vertical bars denote 0.95 confidence intervals

DOI: https://doi.org/10.2478/johr-2021-0007 | Journal eISSN: 2353-3978 (formerly 2300-5009) | Journal ISSN: 2300-5009
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Language: English
Page range: 47 - 54
Submitted on: Nov 1, 2020
Accepted on: Mar 1, 2021
Published on: May 3, 2021
Published by: National Institute of Horticultural Research
In partnership with: Paradigm Publishing Services
Keywords:
cabbage,
explants,
in vitro culture,
regeneration,
trifluralin,
polyploids
Related subjects:
Life sciences,
Biotechnology,
Plant science,
Ecology,
Life sciences, other

© 2021 Oushadee A.J. Abeyawardana, Iva Viehmannová, Martin Koudela, published by National Institute of Horticultural Research
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Volume 29 (2021): Issue 1 (June 2021)
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