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The Effect of Trifluralin on Post-in vitro Morphogenesis of Five Genotypes of Head Cabbage (Brassica oleraceae var. capitata) Cover

The Effect of Trifluralin on Post-in vitro Morphogenesis of Five Genotypes of Head Cabbage (Brassica oleraceae var. capitata)

Journal of Horticultural Research
Volume 29 (2021): Issue 1 (June 2021)
By:
Open Access
|May 2021

Figures & Tables

Figure 1

Organogenesis of cabbage in in vitro cultures: A – shoot induction from cut ends of treated hypocotyl on shoot induction medium in control (bar = 0.5 cm); B – shoot induction from trifluralin treated callus on shoot induction medium (bar = 0.5 cm); C – green dormant callus from treated calli on shoot induction medium (bar = 0.5 cm); D – germinating seeds treated with trifluralin after seven days on seed germination medium (bar = 1 cm); E – growing calli from treated hypocotyls after 14 days on shoot induction medium (bar = 0.5 cm); F – rooted shootlets derived from treated seeds
Organogenesis of cabbage in in vitro cultures: A – shoot induction from cut ends of treated hypocotyl on shoot induction medium in control (bar = 0.5 cm); B – shoot induction from trifluralin treated callus on shoot induction medium (bar = 0.5 cm); C – green dormant callus from treated calli on shoot induction medium (bar = 0.5 cm); D – germinating seeds treated with trifluralin after seven days on seed germination medium (bar = 1 cm); E – growing calli from treated hypocotyls after 14 days on shoot induction medium (bar = 0.5 cm); F – rooted shootlets derived from treated seeds

Figure 2

Effect of cultivar, trifluralin concentration, treatment condition on in vitro culture of seeds and their interaction with each other: A1, B1, C1 – the effect of different treatment conditions on the rate of seed germination, shoot induction, and root induction respectively; A2, B2, C2 – the effect of different cultivars on the rate of seed germination, shoot induction, and root induction respectively; A3, B3, C3 – the effect of different concentrations of trifluralin on seed germination, shoot induction, and root induction respectively. Vertical bars denote 0.95 confidence intervals
Effect of cultivar, trifluralin concentration, treatment condition on in vitro culture of seeds and their interaction with each other: A1, B1, C1 – the effect of different treatment conditions on the rate of seed germination, shoot induction, and root induction respectively; A2, B2, C2 – the effect of different cultivars on the rate of seed germination, shoot induction, and root induction respectively; A3, B3, C3 – the effect of different concentrations of trifluralin on seed germination, shoot induction, and root induction respectively. Vertical bars denote 0.95 confidence intervals

Results of ANOVA to test the influence of cultivar, trifluralin concentration, treatment condition on in vitro culture of cabbage starting from seeds

TraitEffectMSFP
Rate of seed germinationcultivar11339.387.40.0000*
trifluralin concentration33654.1259.30.0000*
treatment condition7613.458.6680.0000*
treatment condition × cultivar638.64.92130.0000*
treatment condition × concentration382.42.94650.0015*
concentration × cultivar925.47.1310.000*
treatment × concentration × cultivar237.41.8290.00262*
Shoots per germinated seedcultivar17.912.8130.014*
trifluralin concentration4.40.6920.5990
treatment condition6.51.0210.3867
treatment condition × cultivar10.051.57950.0799
treatment condition × concentration9.341.46740.1492
concentration × cultivar9.531.49720.0860
treatment × concentration × cultivar6.961.0940.337
Rate of root inductioncultivar10575.347.10.0000*
trifluralin concentration23284.8103.60.0000*
treatment condition6557.229.1860.0000*
treatment condition × cultivar377.31.67930.05559
treatment condition × concentration717.03.19120.00068*
concentration × cultivar1636.57.28420.0000*
treatment × concentration × cultivar446.71.9880.000749*
DOI: https://doi.org/10.2478/johr-2021-0007 | Journal eISSN: 2353-3978 | Journal ISSN: 2300-5009
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Language: English
Page range: 47 - 54
Submitted on: Nov 1, 2020
Accepted on: Mar 1, 2021
Published on: May 3, 2021
Published by: National Institute of Horticultural Research
In partnership with: Paradigm Publishing Services
Publication frequency: 2 times per year
Keywords:
cabbage,
explants,
culture,
regeneration,
trifluralin,
polyploids
Related subjects:
Life sciences,
Biotechnology,
Plant science,
Ecology,
Life sciences, other

© 2021 Oushadee A.J. Abeyawardana, Iva Viehmannová, Martin Koudela, published by National Institute of Horticultural Research
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

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