Influence of Microbiologically Enriched Mineral Fertilizers on Selected Groups of Microorganisms in the Rhizosphere of Strawberry Plants

Sas-Paszt, Lidia; Smolińska, Urszula; Kowalska, Beata; Szczech, Magdalena; Lisek, Anna; Trzciński, Paweł; Głuszek, Sławomir; Górnik, Krzysztof; Derkowska, Edyta; Sumorok, Beata

Influence of Microbiologically Enriched Mineral Fertilizers on Selected Groups of Microorganisms in the Rhizosphere of Strawberry Plants

Volume 29 (2021): Issue 1 (June 2021)

By:

Lidia Sas-Paszt, Urszula Smolińska, Beata Kowalska, Magdalena Szczech, Anna Lisek, Paweł Trzciński, Sławomir Głuszek, Krzysztof Górnik, Edyta Derkowska and Beata Sumorok

Open Access

|May 2021

Figures & Tables

Isolation of actinomycetes and filamentous fungi from soil of strawberry rhizosphere

Treatment no.	Treatments	Actinomycetes A (cfu × 10⁵·g DW⁻¹ of soil)	Actinomycetes B (cfu × 10⁵·g DW⁻¹ of soil)	Filamentous fungi (cfu × 10⁴·g DW⁻¹ of soil)	Actinomycetes A (cfu × 10⁵·g DW⁻¹ of soil)	Actinomycetes B (cfu × 10⁵·g DW⁻¹ of soil)	Filamentous fungi (cfu × 10⁴·g DW⁻¹ of soil)
		2018			2019
1	control	20.0 ± 2.9 b	25.0 ± 3.1 b	15.5 ± 3.0 c	7.7 ± 2.5 d	24.6 ± 6.7 b	20.3 ± 1.1 ab
2	control + NPK	36.9 ± 13.2 b	50.0 ± 15.8 b	11.7 ± 2.7 c	18.6 ± 0.9 bcd	50.6 ± 12.7 ab	22.8 ± 8.8 ab
		+Fungi
3	control + fungi	12.8 ± 0.8 b	16.1 ± 1.1 b	41.5 ± 0.1 a	14.3 ± 1.4 bcd	28.4 ± 1.0 ab	18.5 ± 3.5 ab
4	control + NPK + fungi	54.3 ± 6.5 b	68.3 ± 10.0 b	37.8 ± 3.7 ab	34.8 ± 6.3 abc	52.2 ± 6.1 ab	23.2 ± 5.9 ab
5	urea 100% + fungi	42.7 ± 10.8 b	52.0 ± 15.1 b	30.1 ± 6.8 b	52.1 ± 5.6 a	74.6 ± 8.7 a	30.9 ± 8.0 a
6	urea 60% + fungi	35.9 ± 14.2 b	50.8 ± 19.6 b	46.7 ± 0.7 a	12.7 ± 1.4 bcd	22.8 ± 0.3 b	8.0 ± 0.3 b
		+Bacteria
7	control + bacteria	103.9 ± 7.8 a	110.7 ± 8.5 a	13.5 ± 2.8 c	18.2 ± 1.1 bcd	36.5 ± 0.3 ab	7.8 ± 1.6 b
8	control + NPK + bacteria	34.4 ± 11.7 b	42.5 ± 13.7 b	10.1 ± 2.3 c	11.0 ± 1.7 cd	19.0 ± 1.7 b	12.1 ± 1.3 ab
9	Polifoska 6 100% + bacteria	23.4 ± 6.1 b	36.6 ± 15.0 b	10.3 ± 1.9 c	38.5 ± 0.1 ab	55.1 ± 0.5 ab	22.1 ± 4.5 ab
10	Polifoska 6 100% + bacteria*	21.6 ± 4.7 b	28.3 ± 4.3 b	13.5 ± 0.3 c	18.9 ± 3.4 bcd	27.6 ± 5.4 ab	9.3 ± 2.6 ab
11	Polifoska 6 60% + bacteria	49.9 ± 16.0 b	55.3 ± 15.6 b	18.7 ± 2.8 c	32.1 ± 3.7 abcd	55.8 ± 15.1 ab	15.4 ± 3.3 ab
12	Super Fos Dar 40 100% + bacteria	28.7 ± 5.9 b	33.2 ± 4.5 b	16.7 ± 0.9 c	36.0 ± 11.4 abc	68.1 ± 16.7 ab	18.8 ± 4.3 ab
13	Super Fos Dar 40 60% + bacteria	23.4 ± 1.8 b	32.6 ± 0.2 b	6.2 ± 0.8 c	30.9 ± 12.1 abcd	61.7 ± 19.5 ab	28.3 ± 0.2 ab

Isolation of bacteria from soil of strawberry rhizosphere

Treatment no.	Treatments	Bacteria total count (cfu × 10⁶·g DW⁻¹ of soil)	Pseudomonas total count (cfu × 10³·g DW⁻¹ of soil)	Pseudomonas fluorescens (cfu × 10³·g DW⁻¹ of soil)	Bacteria total count (cfu × 10⁶·g DW⁻¹ of soil)	Pseudomonas total count (cfu × 10³·g DW⁻¹ of soil)	Pseudomonas fluorescens (cfu × 10³·g DW⁻¹ of soil)
		2018			2019
1	control	39.2 ± 7.4 ab	59.4 ± 26.8 a	27.4 ± 10.1 a	13.6 ± 3.7 e	13.9 ± 2.3 b	11.6 ± 1.8 c
2	control + NPK	30.9 ± 2.2 ab	76.5 ± 14.8 a	13.7 ± 3.9 a	7.1 ± 1.1 e	69.9 ± 2.3 a	60.3 ± 2.1 a
		+ Fungi
3	control + fungi	28.4 ± 6,3 ab	42.7 ± 14.1 a	20.6 ± 10.0 a	10.9 ± 0.0 e	9.0 ± 1.9 b	8.3 ± 1.6 c
4	control + NPK + fungi	7.2 ± 1.5 b	61.2 ± 20.3 a	78.9 ± 1.6 a	8.4 ± 0.1 e	42.9 ± 2.9 ab	20.4 ± 1.0 c
5	urea 100% + fungi	43.8 ± 13.6 ab	162.4 ± 58.0 a	78.4 ± 32.0 a	10.1 ± 0.2 e	41.9 ± 5.1 ab	32.4 ± 5.4 bc
6	urea 60% + fungi	61.7 ± 15.3 a	129.6 ± 32.2 a	77.7 ± 24.4 a	54.0 ± 0.6 b	50.1 ± 11.9 ab	26.3 ± 1.2 c
		+ Bacteria
7	control + bacteria	39.7 ± 10.1 ab	100.9 ± 39.5 a	54.2 ± 18.7 a	32.6 ± 6.6 d	35.4 ± 1.8 ab	12.2 ± 2.9 c
8	control + NPK + bacteria	18.2 ± 0.7 b	72.6 ± 29.9 a	48.8 ± 14.4 a	7.4 ± 0.8 e	28.5 ± 6.1 ab	22.9 ± 5. c
9	Polifoska 6 100% + bacteria	25.1 ± 6.7 ab	172.6 ± 54.9 a	24.3 ± 2.9 a	69.3 ± 10.2 a	41.6 ± 8.3 ab	34.6 ± 7.7 bc
10	Polifoska 6 100% + bacteria*	36.9 ± 5.3 ab	80.3 ± 31.2 a	32.0 ± 0.3 a	31.6 ± 1.2 cd	49.3 ± 17.2 ab	15.4 ± 1.6 c
11	Polifoska 6 60% + bacteria	61.5 ± 2.3 a	138.2 ± 41.5 a	47.2 ± 16.1 a	7.7 ± 0.2 e	54.6 ± 14.8 ab	40.0 ± 7.5 abc
12	Super Fos Dar 40 100% + bacteria	34.2 ± 14.5 ab	79.2 ± 30.9 a	54.4 ± 19.7 a	21.2 ± 0.5 de	66.3 ± 18.1 a	56.6 ± 17.1 ab
13	Super Fos Dar 40 60% + bacteria	29.8 ± 2.9 ab	92.9 ± 23.3 a	39.6 ± 12.2 a	43.5 ± 1.0 c	38.1 ± 11.5 ab	19.7 ± 8.0 c

Isolation of phosphorus bacteria from soil of strawberry rhizosphere

Treatment no.	Treatments	Phosphate-solubilizing bacteria A (cfu × 10⁴·g DW⁻¹ of soil)	Phosphate-solubilizing bacteria B (cfu × 10⁴·g DW⁻¹ of soil)	Phosphate-solubilizing bacteria A (cfu × 10⁴·g DW⁻¹ of soil)	Phosphate-solubilizing bacteria B (cfu × 10⁴·g DW⁻¹ of soil)
		2018		2019
1	control	6.8 ± 0.7 bc	51.3 ± 7.1 a	14.6 ± 1.4 bc	36.1 ± 7.5 b
2	control + NPK	9.6 ± 1.8 bc	38.4 ± 0.8 a	28.5 ± 4.5 abc	54.7 ± 10.9 ab
		+Fungi
3	control + fungi	5.3 ± 1.3 bc	49.1 ± 18.4 a	18.9 ± 4.3 bcd	45.0 ± 9.0 ab
4	control + NPK + fungi	13.2 ± 0.8 ab	74.3 ± 21.5 a	31.4 ± 0.8 ab	60.1 ± 3.6 ab
5	urea 100% + fungi	18.6 ± 5.2 ab	56.0 ± 9.3 a	14.8 ± 2.3 bc	34.4 ± 7.5 b
6	urea 60% + fungi	7.0 ± 1.7 bc	66.6 ± 13.9 a	35.2 ± 2.9 a	75.5 ± 5.2 a
		+Bacteria
7	control + bacteria	6.7 ± 0.8 bc	62.9 ± 7.1 a	20.8 ± 3.2 bcd	37.3 ± 4.8 b
8	control + NPK + bacteria	7.0 ± 3.2 bc	48.7 ± 3.2 a	12.5 ± 1.6 d	27.8 ± 2.0 b
9	Polifoska 6 100% + bacteria	9.3 ± 2.0 bc	49.3 ± 6.2 a	25.7 ± 1.5 abc	52.4 ± 4.7 ab
10	Polifoska 6 100% + bacteria*	25.5 ± 0.1 a	52.2 ± 4.7 a	16.6 ± 4.3 cd	31.9 ± 6.7 b
11	Polifoska 6 60% + bacteria	3.0 ± 0.8 c	64.5 ± 6.7 a	20.8 ± 2.0 bcd	51.9 ± 3.3 ab
12	Super Fos Dar 40 100% + bacteria	14.3 ± 5.9 ab	88.0 ± 27.7 a	20.4 ± 3.2 bcd	46.0 ± 13.2 ab
13	Super Fos Dar 40 60% + bacteria	14.0 ± 3.7 ab	54.5 ± 12.6 a	17.8 ± 3.9 bcd	43.1 ± 12.6 ab

The list of treatments (dose of fertilizers per one stoneware)

Treatment no.	Treatments	Mineral fertilizers	Microorganisms
1	control	0	0
2	control + NPK	NPK (12 g Super Fos Dar 40, 50 g potassium salt, 35 g urea before planting and 12 g in midsummer)	0
3	control + fungi	0	Fungi (A. niger and P. lilacinum)
4	control + NPK + fungi	NPK (as in 2)	Fungi (as above)
5	urea 100% + fungi	NPK, except urea in midsummer, 20 g	Fungi (as above)
6	urea 60% + fungi	NPK, except urea before planting, 20 g and 12 g in midsummer	Fungi (as above)
7	control + bacteria	0	Bacteria (Bacillus sp., B. amyloliquefaciens, P. polymyxa added before planting)
8	control + NPK + bacteria	NPK (as in 2)	Bacteria (as above)
9	Polifoska 6 100% + bacteria	Polifoska 6, 26 g, 33 g potassium salt, 30 g urea before planting and 20 g in midsummer	Bacteria (as above, incorporated into the fertilizer during production of granules)
10	Polifoska 6 100% + bacteria*	as in 9	Bacteria (as above but added to the soil before planting)
11	Polifoska 6 60% + bacteria	Polifoska 6 decreased to 14 g and urea decreased to 18 g before planting, 30 g potassium salt	Bacteria (as in 7)
12	Super Fos Dar 40 100% + bacteria	NPK (as in 2)	Bacteria (as in 7)
13	Super Fos Dar 40 60% + bacteria	NPK (as in 2 but Super Fos Dar 40 decreased to 7 g, potassium salt decreased to 30 g and urea decreased to 20 g before planting	Bacteria (as in 7)

DOI: https://doi.org/10.2478/johr-2021-0005 | Journal eISSN: 2353-3978 | Journal ISSN: 2300-5009

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Language: English

Page range: 35 - 46

Submitted on: Aug 1, 2020

Accepted on: Mar 1, 2021

Published on: May 6, 2021

Published by: National Institute of Horticultural Research

In partnership with: Paradigm Publishing Services

Publication frequency: 2 times per year

Keywords:

bio-fertilizers,

rhizosphere of strawberry,

Related subjects:

Ecology,

© 2021 Lidia Sas-Paszt, Urszula Smolińska, Beata Kowalska, Magdalena Szczech, Anna Lisek, Paweł Trzciński, Sławomir Głuszek, Krzysztof Górnik, Edyta Derkowska, Beata Sumorok, published by National Institute of Horticultural Research
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

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