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First Report of Root-Knot Nematode, Meloidogyne incognita, on Tree Houseleek (Aeonium arboreum) in the United States Cover

First Report of Root-Knot Nematode, Meloidogyne incognita, on Tree Houseleek (Aeonium arboreum) in the United States

Journal of Nematology
Volume 57 (2025): Issue 1 (February 2025)
By:
Open Access
|Aug 2025

Figures & Tables

Figure 1:

(A) Amplified products were obtained using M. incognita-specific (MIF/MIR) and M. javanica-specific (Fjav/Rjav) primers. Only the MIF/MIR primers produced a 999-bp band, indicating that the root-knot nematode infecting the Aeonium was M. incognita, while no amplification was observed with the Fjav/Rjav primers. Two mitochondrial DNA regions, covering the intergenic spacer and part of the adjacent large subunit ribosomal RNA gene (lrDNA), were amplified using the TRNAH/MHR106 (approximately 550-bp) and MORF/MTHIS (approximately 750 bp) primer sets; (B) The intergenic spacer, tRNAHis, and the large subunit ribosomal RNA gene (rRNA) were amplified using the C2F3/1108 primers, yielding an approximately 1550-bp product.
(A) Amplified products were obtained using M. incognita-specific (MIF/MIR) and M. javanica-specific (Fjav/Rjav) primers. Only the MIF/MIR primers produced a 999-bp band, indicating that the root-knot nematode infecting the Aeonium was M. incognita, while no amplification was observed with the Fjav/Rjav primers. Two mitochondrial DNA regions, covering the intergenic spacer and part of the adjacent large subunit ribosomal RNA gene (lrDNA), were amplified using the TRNAH/MHR106 (approximately 550-bp) and MORF/MTHIS (approximately 750 bp) primer sets; (B) The intergenic spacer, tRNAHis, and the large subunit ribosomal RNA gene (rRNA) were amplified using the C2F3/1108 primers, yielding an approximately 1550-bp product.

Figure 2:

(A) Tree houseleek (Aeonium arboreum) plant received from Los Angeles, California for diagnosis; (B) Heavy galling on the plant roots, indicating root-knot nematode (RKN) infection; (C) Tomato plants used to test for RKN infection; (D) and (F) Visible galls on the infected tomato roots, both in the pot and after washing; (E) Acid fuchsin staining of the tomato roots, showing the female nematodes inside the roots, along with the morphology of adult and early female nematodes; (G) Cuttings of the Aeonium plants inoculated with nematodes; (H and I) Acid fuchsin staining of the Aeonium arboreum cuttings, showing characteristic symptoms of nematode infection.
(A) Tree houseleek (Aeonium arboreum) plant received from Los Angeles, California for diagnosis; (B) Heavy galling on the plant roots, indicating root-knot nematode (RKN) infection; (C) Tomato plants used to test for RKN infection; (D) and (F) Visible galls on the infected tomato roots, both in the pot and after washing; (E) Acid fuchsin staining of the tomato roots, showing the female nematodes inside the roots, along with the morphology of adult and early female nematodes; (G) Cuttings of the Aeonium plants inoculated with nematodes; (H and I) Acid fuchsin staining of the Aeonium arboreum cuttings, showing characteristic symptoms of nematode infection.
DOI: https://doi.org/10.2478/jofnem-2025-0031 | Journal eISSN: 2640-396X | Journal ISSN: 0022-300X
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Language: English
Submitted on: Jan 23, 2025
Published on: Aug 31, 2025
Published by: Society of Nematologists, Inc.
In partnership with: Paradigm Publishing Services
Publication frequency: 1 times per year
Keywords:
Diagnosis,
California,
mitochondrial haplotyping,
root galls,
root-knot nematode,
species-specific primers
Related subjects:
Life sciences,
Life sciences, other

© 2025 Anil Baniya, Omar Zayed, Perla Achi, Pierluigi Perfetto, Adler Dillman, published by Society of Nematologists, Inc.
This work is licensed under the Creative Commons Attribution 4.0 License.

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