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Development of Monoclonal Antibodies for Identifying Plant-Parasitic Nematodes Cover

Development of Monoclonal Antibodies for Identifying Plant-Parasitic Nematodes

Open Access
|Jul 2025

Figures & Tables

Figure 1:

Indirect ELISA showing the interaction of antigen HG type 0-based monoclonal antibody (mAb) against the four nematode antigen types generated from soybean cyst nematode (SCN) and root-knot nematode (RKN): SCN 1 (HG type 0), SCN 2 (HG type 4.5.7), RKN race 3 and RKN race 4. The concentration of the mAb is in proportion to the template DNA used during the protein synthesis and is therefore in ng/μL.
Indirect ELISA showing the interaction of antigen HG type 0-based monoclonal antibody (mAb) against the four nematode antigen types generated from soybean cyst nematode (SCN) and root-knot nematode (RKN): SCN 1 (HG type 0), SCN 2 (HG type 4.5.7), RKN race 3 and RKN race 4. The concentration of the mAb is in proportion to the template DNA used during the protein synthesis and is therefore in ng/μL.
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Number of monoclonal antibody (mAb) nucleotide sequences generated per antigen type (HG type and race designation for soybean cyst nematode [SCN; Heterodera glycines] and root-knot nematode [RKN; Meloidogyne incognita])_

Antigen typeNo. V(D) J Spanning CellsTotal mAb Sequences
SCN HG type 0323,978
SCN HG type 05
SCN HG type 03,941
SCN HG type 1.2.3.5.6.726,183
SCN HG type 1.2.3.5.6.72,784
SCN HG type 1.2.3.5.6.73,397
RKN race 38,99527,098
RKN race 310,178
RKN race 37,925
RKN race 48,17121,313
RKN race 413,142
DOI: https://doi.org/10.2478/jofnem-2025-0024 | Journal eISSN: 2640-396X | Journal ISSN: 0022-300X
Language: English
Submitted on: Feb 13, 2025
Published on: Jul 19, 2025
Published by: Society of Nematologists, Inc.
In partnership with: Paradigm Publishing Services
Publication frequency: 1 times per year

© 2025 M. Bogale, E. Sampson, W. Hu, A. Baniya, S. Mishra, K. Kwon, H. D. Lopez-Nicora, P. DiGennaro, published by Society of Nematologists, Inc.
This work is licensed under the Creative Commons Attribution 4.0 License.