Table 1:
Number of monoclonal antibody (mAb) nucleotide sequences generated per antigen type (HG type and race designation for soybean cyst nematode [SCN; Heterodera glycines] and root-knot nematode [RKN; Meloidogyne incognita]).
| Antigen type | No. V(D) J Spanning Cells | Total mAb Sequences |
|---|---|---|
| SCN HG type 0 | 32 | 3,978 |
| SCN HG type 0 | 5 | |
| SCN HG type 0 | 3,941 | |
| SCN HG type 1.2.3.5.6.7 | 2 | 6,183 |
| SCN HG type 1.2.3.5.6.7 | 2,784 | |
| SCN HG type 1.2.3.5.6.7 | 3,397 | |
| RKN race 3 | 8,995 | 27,098 |
| RKN race 3 | 10,178 | |
| RKN race 3 | 7,925 | |
| RKN race 4 | 8,171 | 21,313 |
| RKN race 4 | 13,142 |
Figure 1:
Indirect ELISA showing the interaction of antigen HG type 0-based monoclonal antibody (mAb) against the four nematode antigen types generated from soybean cyst nematode (SCN) and root-knot nematode (RKN): SCN 1 (HG type 0), SCN 2 (HG type 4.5.7), RKN race 3 and RKN race 4. The concentration of the mAb is in proportion to the template DNA used during the protein synthesis and is therefore in ng/μL.
