Have a personal or library account? Click to login
Nematocidal activity of fungal filtrates on eggs and juveniles of five species of sedentary endoparasitic nematodes Cover

Nematocidal activity of fungal filtrates on eggs and juveniles of five species of sedentary endoparasitic nematodes

Journal of Nematology
Volume 57 (2025): Issue 1 (February 2025)
By:
Open Access
|Jun 2025

Figures & Tables

Figure 1:

Micrograph of parameters used to differentiate between intact and damaged Heterodera gycines and Globodera ellingtonae eggs. A) 4′,6-diamidino-2-phenylindole (DAPI) stained eggs viewed under the Echo Revolve fluorescent microscope; B) Bright field view with the Olympus microscope; C) Tetramethylrhodamine-5-(and 6)-isothiocyanate (TRITC) view with the Olympus microscope; and D) Fluorescein isothiocyanate (FITC) view with the Olympus microscope (ie = intact eggs, de = damaged eggs, and j2 = second-stage juveniles).
Micrograph of parameters used to differentiate between intact and damaged Heterodera gycines and Globodera ellingtonae eggs. A) 4′,6-diamidino-2-phenylindole (DAPI) stained eggs viewed under the Echo Revolve fluorescent microscope; B) Bright field view with the Olympus microscope; C) Tetramethylrhodamine-5-(and 6)-isothiocyanate (TRITC) view with the Olympus microscope; and D) Fluorescein isothiocyanate (FITC) view with the Olympus microscope (ie = intact eggs, de = damaged eggs, and j2 = second-stage juveniles).

Figure 2:

Effect of fungal filtrates on A) Heterodera glycines and B) Globodera ellingtonae eggs. Bars within a nematode species followed by the same letter are not significantly different from each other (P ≥ 0.05). Control (CTRL), Fusarium acaciae-mearnsii (JB201), Purpureocillium lilacinum (JB209), Trichoderma virens (JB98), Alternaria tenuissima (JB217), Aureobasidium sp. (JB70), and Fusarium proliferatum (JB173).
Effect of fungal filtrates on A) Heterodera glycines and B) Globodera ellingtonae eggs. Bars within a nematode species followed by the same letter are not significantly different from each other (P ≥ 0.05). Control (CTRL), Fusarium acaciae-mearnsii (JB201), Purpureocillium lilacinum (JB209), Trichoderma virens (JB98), Alternaria tenuissima (JB217), Aureobasidium sp. (JB70), and Fusarium proliferatum (JB173).

Figure 3:

Effect of fungal filtrates on A) Globodera ellingtonae (Ge), B) Meloidogyne chitwoodi (Mc), C) M. hapla (Mh), and D) M. incognita (Mi) second-stage juvenile (J2) mortality. Bars within a nematode species followed by the same letter are not significantly different from each other (P ≥ 0.05). Control (CTRL), Fusarium acaciae-mearnsii (JB201), Purpureocillium lilacinum (JB209), Trichoderma virens (JB98), and Alternaria tenuissima (JB217).
Effect of fungal filtrates on A) Globodera ellingtonae (Ge), B) Meloidogyne chitwoodi (Mc), C) M. hapla (Mh), and D) M. incognita (Mi) second-stage juvenile (J2) mortality. Bars within a nematode species followed by the same letter are not significantly different from each other (P ≥ 0.05). Control (CTRL), Fusarium acaciae-mearnsii (JB201), Purpureocillium lilacinum (JB209), Trichoderma virens (JB98), and Alternaria tenuissima (JB217).

Fungi isolated from cyst nematodes initially screened for impacts on egg viability of Globodera ellingtonae (Ge) and Heterodera glycines (Hg)_ More information on isolation of these strains can be found in Jumbam et al_ (2024)_

Fungal SpeciesStrainSourceaSitebCountry% viablec
HgGe
Alternaria sp.JB176GpGHUSA-53
A. tenuissimaJB217HgFDUSA0-
Arthopyrenia salicisJB155GrGHFrance670
Aureobasidium sp.MCA7097AirFDPeru-17
Aureobasidium sp.JB70GeFDUSA-29
Cephalotrichum microsporumJB178GrGHFrance-20
Cladorrhinum samalaJB211HgFDUSA67-
C. cladosporioidesJB93GeFDUSA-46
C. herbarumJB179GpGHFrance-18
Cladosporium sp.JB110GpGHUK-42
Colletotrichum nigrumJB115GrGHUSA100-
Fusarium acacia-mearnsiiJB201HgFDUSA0-
F. culmorumJB121HaGHUSA69-
F. equisetiJB214HgFDUSA80-
F. equisetiJB126GrFDUSA-36
F. oxysporiumJB213HgFDUSA8150
F. proliferatumJB173GpGHUSA821
F. pseudograminearumJB276GsFDPeru-2
F. redolensJB280GsFDPeru-8
Fusarium sp.JB266GsFDPeru 48
F. tricinctumJB124HaGHUSA57-
Geomyces sp.1JB219HaFDUSA100-
Geomyces sp.2JB220HaFDUSA67-
Humicola griseaJB275GsFDPeru-48
Keithomyces carneusJB282GsFDPeru-37
Neocosmospora solaniJB123GrGHFrance6746
N. solaniJB202HgFDUSA60-
Penicillium janthinellumJB122GrFDUSA-10
P. rubensJB270GsFDPeru-47
Penicillium sp.JB204HgFDUSA0-
Purpureocillium lilacinumJB209HgFDUSA83-
Thielavia sp.JB104HaGHUSA100-
Trichocladium griseumJB274GsFDPeru-47
Trichoderma sp.1JB96GrGHUSA41-
Trichoderma sp.2JB98HaGHUSA1128
T. virensJB101GrGHFrance7145
Unknown1JB240HaFDUSA70-
Unknown2JB243HaFDUSA87-
Unknown3JB245HaFDUSA0-
Unseq2JB278GrFDUSA-42
Unseq3JB285GsFDPeru-35
Zopfiella longicaudataJB100GpFDFrance-45
Control----7351

Top six fungi retained for fungal filtrate assays against Heterodera glycines (Hg), Globerdera ellingtonae (Ge), Meloidogyne chitwoodi (Mc), M_ hapla (Mh), and M_ incognita (Mi) eggs or second-stage juveniles (J2)_

StrainFungal SpeciesNematodeLife stage assayed
JB70Aureobasidium sp.Geeggs
JB98Trichoderma virensGe, Hgeggs
Ge, Mc, Mh, MiJ2
JB173Fusarium proliferatumGeeggs
JB201F. acaciae-mearnsiiHgeggs
Ge, Mc, Mh, MiJ2
JB209Purpureocilium lilacinumGe, Hgeggs
Ge, Mc, Mh, MiJ2
JB217Alternaria tenuissimaHgeggs
Ge, Mc, Mh, MiJ2
DOI: https://doi.org/10.2478/jofnem-2025-0018 | Journal eISSN: 2640-396X | Journal ISSN: 0022-300X
Journal RSS Feed
Language: English
Submitted on: Feb 12, 2025
Published on: Jun 4, 2025
Published by: Society of Nematologists, Inc.
In partnership with: Paradigm Publishing Services
Publication frequency: 1 times per year
Keywords:
Biological control,
cyst nematodes,
fungal filtrates,
root knot nematodes,
secondary metabolites
Related subjects:
Life sciences,
Life sciences, other

© 2025 B. Jumbam, A. B. Peetz, V. S. Kunwar, L. Zhang, I. A. Zasada, M. C. Aime, published by Society of Nematologists, Inc.
This work is licensed under the Creative Commons Attribution 4.0 License.

Previous articleVolume 57 (2025): Issue 1 (February 2025)Next article