Figure 1:
Line drawings of Deladenus uljinensis n. sp. (A–I): A: Female whole body; B: Male whole body; C: Female reproductive system; D: Female anterior region; E: Male anterior region; F, G: Female tail region; H: Lateral field; I: Male tail region.
Figure 2:
Photomicrographs of Deladenus uljinensis n. sp. (A–K). A, B: Female reproductive system; C: Female anterior region; D: Female pharyngeal and glandular region; E: Male anterior region; F: Excretory pore / hemizonid position; G–I: Variation in female tail region; J: Lateral field; K: Male tail region. The arrow labeled n indicates the position of glandular nucleus. All other arrows indicate the collective position of both the excretory pore and the hemizonid (Scale bars: A = 50 μm; B, C, D, E, G, H, I and K = 20 μm; F and J = 10 μm).
Table 1:
Morphometrics of mycetophagous population of Deladenus uljinensis n. sp. and Deladenus posteroporus from Korea.
| Character | Deladenus uljinensis n. sp. | Deladenus posteroporus | |||
|---|---|---|---|---|---|
| Holotype ♀ | ♀♀ | ♂♂ | ♀♀ | ♂♂ | |
| n | – | 30 | 15 | 18 | 10 |
| L | 681.0 | 641.6±53.1 (504.0–736.0) | 524.2±24.9 (482.0–561.0) | 1007.7±156.3 (819.0–1199.0) | 1039.8±62.3 (943.0–1150.0) |
| a | 26.2 | 24.1±1.9 (21.1–28.3) | 24.9±2.4 (21.3–29.3) | 45.4±4.9 (36.2–52.6) | 48.8±3.0 (42.3–51.7) |
| b | 10.1 | 9.3±0.7 (7.7–11.0) | 8.0±0.9 (6.7–9.3) | 11.2±1.4 (9.4–13.7) | 11.1±0.7 (10.1–11.9) |
| b′ | 4.7 | 4.5±0.4 (3.7–5.2) | 3.9±0.4 (3.5–4.6) | 5.4±0.4 (4.6–6.0) | 5.6±0.3 (5.0–6.0) |
| c | 21.0 | 20.8±1.7 (17.5–24.3) | 17.8±1.6 (14.8–19.9) | 36.2±6.5 (30.0–49.1) | 25.5±1.5 (23.4–27.2) |
| c′ | 1.9 | 2.0±0.2 (1.7–2.4) | 2.3±0.3 (2.0–3.2) | 2.2±0.3 (1.7–2.8) | 2.9±0.3 (2.6–3.4) |
| V | 89.9 | 90.4±0.7 (88.6–91.7) | – | 93.0±1.2 (90.9–94.3) | – |
| G1/T | 67.0 | 60.6±5.5 (52.6–71.5) | 58.4±3.3 (53.0–66.4) | 60.1±8.2 (51.3–74.3) | 55.0±5.1 (44.8–58.6) |
| Lip diam. | 6.0 | 6.6±0.5 (5.0–7.0) | 5.6±0.3 (5.0–6.5) | 6.3±0.5 (5.0–7.0) | 6.0±0.3 (5.5–6.5) |
| Stylet length | 8.5 | 8.8±0.3 (8.0–9.5) | 8.5±0.3 (8.0–9.0) | 9.6±0.4 (8.5–10.0) | 9.6±0.3 (9.0–10.0) |
| Anterior to nerve ring | 60.0 | 62.3±4.8 (55.0–72.0) | 58.5±5.8 (50.0–70.5) | 79.2±8.4 (66.5–99.0) | 82.5±6.5 (71.0–90.0) |
| Oesophageal-intestinal junction | 67.0 | 69.2±5.3 (60.5–80.5) | 65.9±6.4 (56.0–78.0) | 89.7±7.9 (77.5–109.5) | 93.7±8.1 (82.0–105.5) |
| Anterior to Hemizonid | 100.5 | 99.0±5.5 (90.0–109.5) | 89.0±3.9 (82.5–95.5) | 110.5±7.9 (97.0–127.5) | 111.0±6.0 (99.5–118.5) |
| Anterior to excretory pore | 102.0 | 102.0±5.5 (91.0–111.0) | 91.3±4.2 (83.0–98.0) | 111.6±8.2 (98.0–132.0) | 112.5±6.9 (102.0–126.0) |
| Glandular overlap | 76.0 | 72.4±7.0 (57.0–84.0) | 69.2±8.4 (52.0–82.5) | 97.5±20.9 (70.0–122.0) | 91.8±14.0 (80.0–109.5) |
| Maximum body diam. | 26.0 | 26.7±2.8 (21.5–33.0) | 21.2±1.4 (18.5–23.0) | 22.4±3.7 (16.5–30.0) | 21.4±1.8 (18.5–25.0) |
| Vulval body diam. | 24.5 | 22.3±2.2 (16.0–26.5) | – | 19.5±4.4 (13.0–26.0) | – |
| Vulva to anus distance (VA) | 33.5 | 29.4±3.4 (21.5–37.0) | – | 39.9±4.1 (34.0–49.0) | – |
| Anal / cloacal body diam. | 17.0 | 15.6±1.4 (12.5–18.5) | 12.8±0.8 (11.0–14.0) | 12.6±1.3 (10.5–15.0) | 14.0±0.4 (13.5–15.0) |
| Tail length | 32.5 | 30.9±2.2 (26.0–36.0) | 29.7±3.1 (24.5–35.0) | 28.2±4.1 (22.5–35.0) | 40.9±3.6 (36.5–46.5) |
| Ratio of VA to tail length | 1.0 | 1.0±0.1 (0.7–1.2) | – | 1.5±0.3 (1.0–2.0) | – |
| Spicules | – | – | 19.4±1.0 (18.5–21.5) | – | 21.9±1.1 (20.0–23.0) |
| Gubernaculum | – | – | 5.6±0.4 (5.0–6.0) | – | 6.1±0.7 (5.0–7.0) |
Figure 3:
Photomicrographs of Deladenus posteroporus (A–J). A: Female anterior region; B: Male anterior region; C: Female pharyngeal and glandular region; D: Excretory pore / hemizonid position; E–G: Variation in female tail region; H: Lateral field; I: Male tail region; J: Vulval region with spermatheca. The two arrows labeled h and e indicate the position of hemizonid and excretory pore, respectively (Scale bars: A–I = 20 μm; J = 10 μm).
Figure 4:
Bayesian tree inferred under the GTR + I + G model from 18S-rRNA gene sequences of Deladenus spp. and other related species from various genera. Posterior probability values exceeding 50% are given on appropriate clades. The studied population is indicated in bold text. Outgroup taxa: Acrobeloides varius and Cephalobus cubaensis.
Figure 5:
Bayesian tree inferred under the GTR + I + G model from LSU D2–D3 partial sequences of Deladenus spp. and other related species from various genera. Posterior probability values exceeding 50% are given on appropriate clades. The studied population is indicated in bold text. Outgroup taxa: Oscheius myriophilus and Poikilolaimus oxycercus.
Figure 6:
Bayesian tree inferred under the GTR + I + G model from COI gene sequences of Deladenus spp. and other related species from various genera. Posterior probability values exceeding 50% are given on appropriate clades. The studied population is indicated in bold text. Outgroup taxa: Ektaphelenchus joyceae and Devibursaphelenchus eproctatus.