Figure 1:
Figure 2:
Figure 3:
Morphometric characteristics of Bursaphelenchus xylophilus (Armenia, env_ Hankavan vill_, collected in 2023) in comparison with the typical populations from USA, Japan, and Portugal_
| Males | Females | |||||||
|---|---|---|---|---|---|---|---|---|
| Character | Armenia this study | USA (Nickle et al., 1981) | Japan (Mamiya and Kiyohara, 1972) | Portugal (Mota et al., 1999) | Armenia this study | USA (Nickle et al., 1981) | Japan (Mamiya and Kiyohara, 1972) | Portugal (Mota et al., 1999) |
| L, μm | 0.85 | 0.56 (0.52–0.60) | 0.73 (0.59–0.82) | 1.03 (0.80–1.30) | 0.98 (0.97–1.00) | 0.52 (0.45–0.61) | 0.81 (0.71–1.01) | 1.05 (0.89–1.29) |
| Stylet, μm | 11.9 | 13.3 (12.6–13.8) | 14.9 (14.0–17.0) | 12.6 (11.0–16.0) | 12.4 (12.7–12.1) | 12.8 (12.6–13.0) | 15.9 (14.0–18.0) | 12.3 (11.0–15.0) |
| Spicule, μm | 24.8 | 21.2 (18.8–23.0) | 27.0 (25.0–30.0) | 24.0 (22.0–25.0) | - | - | - | - |
| Vulva position, % of L | - | - | - | - | 75.4 (75.0–75.7) | 74.7 (73.0–78.0) | 72.7 (67.0–78.0) | 73.3 (70.0–76.0) |
Specimens of the Bursaphelenchus spp_ and outgroup sample used in phylogenetic analysis_
| Species | Country | Plant host | GenBank Accession No. mtCOI | Reference |
|---|---|---|---|---|
| B. xylophilus | China | Pinus koraiensis | MT808403 | GenBank |
| China | Pinus koraiensis | MT808404 | ||
| China | Pinus tabuliformis | MT808405 | ||
| USA* | Pinus packaging wood | JF317257 | Gu et al., 2011 | |
| Japan | Unknown | LC316944 | GenBank | |
| Japan | Unknown | LC311456 | GenBank | |
| Japan | Pinus spp. | AB067766 | Kanzaki and Futai, 2002 | |
| Portugal | Pinus pinaster | JQ514068 | Moreira et al., 2014 | |
| JQ429761 | ||||
| South Korea | Unknown | GQ332424 | Sultana et al., 2013b | |
| Armenia | Pinus sylvestris | PP989924 | This study | |
| B. mucronatus | South Korea | Unknown | NC021120 | Sultana et al., 2013a |
| South Korea | Unknown | GU177865 | Sultana et al., 2013b | |
| Japan | Pinus spp. | AB067765 | Kanzaki and Futai, 2002 | |
| Japan | Unknown | AB595193 | GenBank | |
| Japan | Pinus thunbergii | AB634848 | Matsunaga and Togashi, 2004 | |
| Japan | Pinus thunbergii | AB634847 | Matsunaga and Togashi, 2004 | |
| Japan | Unknown | AY508049 | Ye et al., 2007 | |
| Ukraine* | Pinus packaging wood | JF317258 | Gu et al., 2011 | |
| Brazil | Pinus packaging wood | JF317259 | Gu et al., 2011 | |
| Finland | Pinus sylvestris | AY508051 | Ye et al., 2007 | |
| B. sexdentati | Germany | Pinus sylvestris | MN101135 | Gu et al., 2020 |
| Greece | Pinus nigra | AY508063 | Ye et al., 2007 | |
| Aphelenchoides besseyi | USA | Fragaria ananassa | AY508072 | Ye et al., 2007 |