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Summary information of single nucleotide variations (SNV) and/or single nucleotide polymorphism (SNPs) analysis in this study for identification and genetic diversity of Tylenchulus semipenetrans populations from Fars province, Iran_
| Locus | No. of sites | Segregating sites or SNVs | SNPs | No. of haplotypes | Nucleotide diversity | Tajima’s D | Sequence conservation | Min recombination |
|---|---|---|---|---|---|---|---|---|
| COI | 603 | 16 | 11 | 10 | Pi: 0.00767 | D: 0.89661 | C: 0.973 | 1 |
| D2D3 | 676 | 24 | 12 | 33 | Pi: 0.00535 | D: −1.02740 | C: 0.964 | 6 |
| ITS | 597 | 17 | 11 | 16 | Pi: 0.00515 | D: −0.89706 | C: 0.972 | 2 |
The primers used in this study for identification and genetic diversity of Tylenchulus semipenetrans populations_
| Primer code | Primer sequence (5′-3′) | Product size (bp) | Target region | References |
|---|---|---|---|---|
| 18S | TTGATTAGGTCCCTGCCCTTT | 967 | ITS1-5.8S-ITS2 | Marek et al., 2010 |
| 21S | TTTCACTCGCCGTTACTAAGG | |||
| TW81F | GTTTCCGTAGGTGAACCTGC | 809–841 | ITS1-5.8S-ITS2 | Tanha Maafi et al., 2003 |
| AB28R | ATATGCTTAAGTTCAGCGGGT | |||
| Ts2-IF | TTCGAGAAACTTGGGGATTGGC | 770 | ITS1-5.8S-ITS2 | Present study |
| Ts2-IR | CAGGGACCTATGATCAAGTGCT | T. semipenetrans specific | ||
| D2AF | ACAAGTACCGTGAGGGAAAGTTG | 774–777 | 28S D2-D3 | Subbotin et al., 2006 |
| D3BR | TCGGAAGGAACCAGCTACTA | |||
| COI-F5 | AATWTWGGTGTTGGAACTTCTTGAAC | 790 | Cytochrome oxidase subunit I | Powers et al., 2014 |
| COI-R9 | CTTAAAACATAATGRAAATGWGCWACW | |||
| ACATAATAAGTATC- |
Morphometrics of the second-stage juveniles of 31 populations (five specimens each) of Tylenchulus semipenetrans, collected from citrus orchards of Fars Province, Iran_ Data are expressed as mean ± standard deviation (range) of population means or specimens_ Measurements are in μm_
| Character/Population code | Means of populations | Specimens |
|---|---|---|
| n | 31 | 154 |
| L | 331 ± 12.1 (304–349) | 332 ± 18 (276–376) |
| a | 27.6 ± 1.1 (24.3–29.7) | 28 ± 2 (22.4–34.7) |
| b | 3.5 ± 0.1 (3.2–3.7) | 3.5 ± .2 (3.1–4.1) |
| Stylet | 11.4 ± 0.3 (11–11.8) | 11.4 ± .4 (10.4–12.4) |
| Conus | 6.1 ± 0.2 (5.5–6.7) | 6.1 ± .4 (4.8–7.3) |
| Anterior end to the center of the median bulb | 46.7 ± 1.6 (43.4–49.5) | 46.8 ± 2.4 (39.9–53) |
| Pharynx length | 94 ± 3.7 (85–103) | 95 ± 5 (76–106.5) |
| MB | 49.3 ± 1.2 (45.5–51) | 49 ± 2 (41–55) |
| Anterior end to hemizonid | 66.1 ± 2.2 (61.3–70.4) | 66 ± 3.4 (58–77) |
| Anterior end to secretory-excretory pore (S. E. pore) | 183 ± 7.5 (168–196) | 183 ± 12 (147–222) |
| Anterior end to nerve ring | 61.1 ± 2.3 (57.1–66.6) | 61 ± 3 (51–71) |
| S. E. pore to genital primordium (GP) | 19.4 ± 2.8 (12.8–24.9) | 20 ± 4.2 (12–32) |
| Anterior end to GP | 200 ± 6.7 (184–212) | 200 ± 10 (165–222) |
| GP length | 12.6 ± 1.6 (9.8–17.4) | 13 ± 2 (8.5–21) |
| GP to the posterior end | 118 ± 7.4 (101–130) | 119 ± 11.7 (90–155) |
| Excretory pore from the anterior end (% of L) | 56.2 ± 2.1 (53.2–60.4) | 56 ± 3 (47–64) |
| Anterior end to GB (% of L) | 60.6 ± 1.3 (58.4–63.2) | 60 ± 2 (54–67) |
| Median bulb width | 6.2 ± 0.3 (5.4–6.6) | 6 ± .5 (4.9–7.5) |
| Median bulb length | 13.2 ± 0.9 (11.9–16) | 13 ± 1.4 (10–18) |
| Median bulb length/diam. ratio | 211 ± 16.5 (182–262) | 212 ± 26 (104–282) |
| body width (BW) in Pharynx | 11.2 ± 0.4 (10.3–11.9) | 11 ± .6 (9.6–13) |
| Max. BW | 12.0 ± 0.4 (10.7–12.6) | 12 ± .6 (10–14) |
| M | 0.5 ± 0.02 (0.5–0.6) | .5 ± 0 (.4–.7) |
| Stylet / L (%) | 3.5 ± 0.1 (3.2–3.9) | 3.4 ± .2 (3–4) |
| S. E. pore / L (%) | 55.4 ± 2.1 (51.8–60) | 55 ± 3 (47–64) |
The sampling sites of Tylenchulus semipenetrans the corresponding GenBank accession numbers for ITS, D2-D3 expansion segments of 28S and COI mtDNA sequences obtained in this study_
| Soil sample codes | GPS location | Locality | Host (Citrus spp.) | rDNA genes | Mt DNA COI | ||
|---|---|---|---|---|---|---|---|
| latitude | longitude | D2–D3 | ITS | ||||
| 25 | 28.6694 | 53.60647 | Qotb Abad, Jahrom | C. limetta | OP723626 | OP722727 | OP739535 |
| 32 | 28.52135 | 53.67221 | Jahrom | C. sinensis | OP723604 | OP722708 | OP739514 |
| 42 | 29.57487 | 51.73012 | Ahmad Abad, Kazerun | C. sinensis | OP723627 | OP722728 | OP739536 |
| 112 | 28.95546 | 53.60143 | Phase-e5, Fasa | C. sinensis | OP723629 | OP722729 | OP739537 |
| 411 | 29.62299 | 51.58779 | Hasan Abad, Kazerun | C. sinensis | OP723616 | OP722718 | OP739524 |
| 678 | 28.99909 | 53.12713 | Aliabad, Khafr | C. aurantium | OP723630 | OP722730 | - |
| 682 | 28.98881 | 53.15637 | Balashahr, Khafr | C. sinensis | - | - | - |
| 698 | 28.51967 | 53.60683 | Heydarabad, Jahrom | C. limetta | OP723597 | - | OP739507 |
| 707 | 28.53547 | 53.65353 | Najib Abad, Jahrom | C. aurantium | OP723610 | OP722704 | OP739510 |
| 710 | 28.5391 | 53.53022 | Maghsudabad, Jahrom | C. limetta | OP723606 | OP722696 | OP739515 |
| 712 | 28.5397 | 53.53049 | Maghsudabad, Jahrom | C. aurantium | OP723631 | OP722731 | OP739538 |
| 716 | 28.47551 | 53.49098 | Mill, Jahrom | C. aurantium | OP723607 | OP722710 | OP739516 |
| 717 | 28.66863 | 53.60608 | Qotb Abad, Jahrom | C. limetta | OP723591 | OP722698 | OP739501 |
| 720 | 28.66878 | 53.6059 | Yousofabad, Jahrom | C. limetta | OP723592 | OP722699 | OP739502 |
| 733 | 28.93138 | 53.60712 | Kazemabad, Fasa | C. sinensis | OP723601 | OP722705 | OP739511 |
| 735 | 28.97311 | 53.63675 | Banyan, Fasa | C. sinensis | OP723613 | OP722715 | OP739521 |
| 737 | 28.95768 | 53.5981 | Phase-e5, Fasa | C. sinensis | OP723614 | OP722716 | OP739522 |
| 743 | 29.03657 | 53.64304 | Akbarabad, Fasa | C. bigaradia | OP723615 | OP722717 | OP739523 |
| 746 | 28.668855 | 54.665161 | Bagh-e Morakabat, Darab | C. sinensis | OP723628 | OP722722 | OP739529 |
| 749 | 28.72201 | 54.57227 | Naghsh Shapour, Darab | C. sinensis | OP723632 | OP722732 | OP739539 |
| 755 | 28.67927 | 54.65618 | Jannat Shahr, Darab | C. bigaradia | OP723639 | OP722738 | OP739546 |
| 759 | 28.64105 | 54.64284 | Deh Kheyr Payin, Darab | C. sinensis | OP723633 | OP722733 | OP739540 |
| 763 | 28.75331 | 54.44562 | Sharak-e Sarollah, Darab | C. sinensis | OP723618 | OP722719 | OP739526 |
| 765 | 28.95749 | 53.60026 | Hasan Abad, Darab | C. limetta | OP723634 | - | OP739541 |
| 771 | 28.786689 | 54.339938 | Fasarood, Darab | C. aurantium | OP723593 | OP722700 | OP739503 |
| 772 | 28.687654 | 54.647131 | Zein Abad Sangi, Darab | C. sinensis | OP723608 | OP722712 | OP739517 |
| 773 | 28.76994 | 54.22515 | Eij, Darab | C. sinensis | OP723602 | OP722706 | OP739512 |
| 777 | 28.445396 | 53.042906 | Gandoman, Karzin | C. reticulata | OP723594 | OP722701 | OP739504 |
| 780 | 28.442301 | 53.143399 | Emam Shahr, Ghir | C. limetta | OP723596 | OP722702 | OP739506 |
| 785 | 28.34352 | 53.25282 | Tang-e Ruein, Ghir | C. aurantium | OP723636 | OP722735 | OP739543 |
| 789 | 28.452529 | 53.127036 | Deh Beh, Ghir | C. aurantium | OP723609 | OP722711 | OP739518 |
| 793 | 28.285395 | 53.074062 | Mand, Karzin | C. bigaradia | OP723619 | OP722720 | OP739527 |
| 795 | 28.328269 | 53.038029 | Eslam Abad, Karzin | C. aurantium | OP723620 | OP722721 | OP739528 |
| 801 | 29.56717 | 51.75703 | Ahmadabad, Kazerun | C. aurantium | OP723611 | OP722713 | OP739519 |
| 802 | 29.56924 | 51.75969 | Ahmadabad, Kazerun | C. aurantium | OP723637 | OP722736 | OP739544 |
| 812 | 29.79435 | 51.57338 | Ganjeii, Kazerun | C. sinensis | OP723640 | OP722739 | OP739547 |
| 818 | 29.7594 | 51.55155 | Sheykhi, Kazerun | C. sinensis | OP723642 | OP722741 | OP739549 |
| 821 | 29.72973 | 51.53522 | Anarestan, Kazerun | C. sinensis | OP723612 | OP722714 | OP739520 |
| 882 | 29.56109 | 51.77738 | Zavali, Kazerun | C. aurantium | OP723638 | OP722737 | OP739545 |
| 908 | 29.00358 | 53.11102 | Karadeh, Khafr | C. aurantium | OP723603 | OP722707 | OP739513 |
| 921 | 28.92434 | 53.33666 | Khavaran, Khafr | C. sinensis | OP723641 | OP722740 | OP739548 |
| 740-2 | 28.89014 | 53.68479 | Dastjeh, Fasa | C. sinensis | OP723617 | - | OP739525 |
| Ami | 28.96260 | 54.04458 | Darab | C. aurantium | OP723621 | OP722723 | OP739530 |
| ARE | 29.61910 | 52.57446 | Sardaran, Shiraz | C. limetta | OP723635 | OP722734 | OP739542 |
| Beh | 28.47110 | 53.03187 | Karzin | C. aurantium | OP723622 | OP722724 | OP739531 |
| Behz-Greenhouse | 29.63544 | 52.52485 | Eram Garden, Shiraz | C. aurantium | OP723623 | OP722725 | OP739532 |
| Sh1 | 36.613791 | 53.258824 | Behshahr1, Mazandaran | C. sinensis | OP723624 | - | OP739533 |
| Sh2 | 36.625476 | 52.931576 | Behshahr2, Mazandaran | C. sinensis | OP723625 | OP722726 | OP739534 |
Single nucleotide polymorphism in the alignment of the citrus nematode (Tylenchulus semipenetrans) ITS of rDNA gene partial sequences_
| ITS haplotypes | Isolate(s) | Position of the single nucleotide variations/polymorphisms (SNVs/SNPs) on the sequences | ||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 32 | 37 | 46 | 71 | 92 | 122 | 139 | 151 | 159 | 161 | 162 | 188 | 225 | 354 | 405 | 423 | 523 | ||
| T. semipenetrans JN112270.1 | CD1_cl2 | A | G | G | T | C | A | G | C | A | C | T | T | C | T | C | C | C |
| TsA (n = 20) | 37, 42, 112, 678, 706, 716, 717, 733, 743, 755, 763, 777, 778, 789, 795, 821,882, 921 BEHZ & SH2 | A | G | G | T | C | A | G | C | A | C | T | T | C | T | C | C | C |
| TsB (n = 6) | 707, 710, 754, 801, 818 & ARE | C | A | G | A | C | A | G | C | A | C | T | T | C | T | C | C | C |
| TsC (n = 1) | 812 | C | A | G | T | C | A | G | C | A | C | C | T | C | T | C | C | C |
| TsD (n = 3) | 720, 773 & 802 | C | A | A | A | C | A | C | C | A | C | T | T | C | T | C | C | C |
| TsE (n = 1) | 785 | C | A | G | A | C | A | A | C | A | C | T | C | T | T | C | C | C |
| TsF (n = 1) | 759 | C | A | G | A | T | A | A | C | A | C | T | C | C | T | C | C | C |
| TsG (n = 6) | 712, 735, 737, 749, 771, & 908 | A | G | G | T | C | A | G | C | A | C | C | T | C | T | C | C | C |
| TsH (n = 1) | 25 | C | A | G | A | T | A | A | C | A | T | T | C | C | T | C | C | C |
| TsI (n = 1) | BEH | A | G | G | T | C | A | G | C | A | C | T | T | T | T | C | C | C |
| TsJ (n = 1) | AMI | A | G | G | T | C | A | G | C | A | C | T | C | C | T | C | C | C |
| TsK (n = 1) | 746 | C | A | G | A | T | A | A | C | A | C | T | C | C | A | T | C | T |
| TsL (n = 1) | 793 | C | A | G | A | C | A | G | C | G | C | T | T | C | T | C | C | C |
| TsM (n = 1) | 411 | C | A | G | T | T | A | A | C | A | C | T | C | C | T | C | C | C |
| TsN (n = 1) | 772 | C | A | G | T | C | A | G | T | T | C | T | T | C | T | C | T | T |
| TsO (n = 1) | 32 | C | A | G | A | T | G | A | C | A | C | T | C | C | T | C | C | C |
| TsP (n = 1) | 780 | C | G | G | T | C | A | G | C | A | C | C | T | C | T | C | C | C |
Single nucleotide polymorphism in the alignment of the citrus nematode (Tylenchulus semipenetrans) D2-D3 of 28 S rDNA gene_
| D2D3 haplotypes | Isolate(s) | Position of the single nucleotide variations/polymorphisms (SNVs/SNPs) on the sequences | |||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 34 | 55 | 59 | 73 | 84 | 94 | 104 | 113 | 119 | 125 | 157 | 167 | 223 | 238 | 260 | 263 | 340 | 355 | 369 | 456 | 497 | 502 | 616 | 661 | ||
| T. semipenetrans KM598334.1 | ES-Jirof2 | G | C | T | C | C | T | A | G | G | A | C | G | T | T | C | C | G | C | G | A | C | T | G | A |
| Ts1 (n = 6) | 720, 773, 32, 759, 765 & 818 | G | C | T | C | C | T | A | G | G | A | C | G | T | T | C | C | G | C | G | A | C | T | G | A |
| Ts2 (n = 2) | BEH & 921 | G | C | T | C | T | T | A | G | G | A | C | C | T | T | C | C | T | C | G | A | T | T | G | A |
| Ts3 (n = 2) | AMI & 812 | G | C | T | C | T | T | A | G | G | A | C | C | T | T | C | C | T | C | G | A | C | T | G | A |
| Ts4 (n = 1) | 755 | G | C | T | C | C | T | A | G | G | A | C | G | G | C | C | C | G | C | G | G | C | T | G | A |
| Ts5 (n = 1) | 882 | G | C | T | C | C | T | A | G | G | A | C | G | T | C | C | C | G | C | G | G | C | T | A | A |
| Ts6 (n = 1) | 802 | G | C | T | C | T | T | A | G | G | A | C | G | T | T | C | C | T | C | T | A | C | T | G | A |
| Ts7 (n = 1) | 785 | G | C | T | C | C | T | A | A | G | A | C | G | T | T | C | C | G | C | G | A | C | T | G | A |
| Ts8 (n = 1) | ARE | G | C | T | C | T | T | A | G | G | A | C | C | T | T | C | C | T | T | G | A | T | T | G | A |
| Ts9 (n = 2) | 740 & 749 | G | C | T | C | C | T | A | G | G | G | C | G | T | T | C | C | G | C | G | A | C | T | G | A |
| Ts10 (n = 1) | 712 | G | C | T | C | C | T | A | G | G | G | T | G | T | T | C | C | G | C | G | A | C | T | G | A |
| Ts11 (n = 1) | 678 | G | C | T | C | T | T | A | G | G | A | C | G | T | C | C | C | G | C | G | A | C | T | G | A |
| Ts12 (n = 2) | 112 & 777 | G | C | T | C | T | T | A | G | G | A | C | C | T | T | C | T | T | C | G | A | T | T | G | A |
| Ts13 (n = 1) | 746 | G | C | T | C | C | T | T | G | G | G | T | G | T | T | C | C | G | C | G | A | C | T | G | A |
| Ts14 (n = 1) | 42 | G | C | T | C | T | T | A | G | G | A | C | G | T | T | C | C | T | C | G | A | C | T | G | A |
| Ts15 (n = 1) | 25 | G | C | T | C | C | T | A | G | T | G | C | G | T | T | C | C | G | C | G | A | C | T | G | A |
| Ts16 (n = 9) | 717, 698, 706, 716, 789, 821, 743, 763 & SH2 | G | C | T | C | C | T | A | G | G | A | C | G | T | C | C | C | G | C | G | G | C | T | G | A |
| Ts17 (n = 2) | 733 & SH1 | G | C | T | C | C | T | A | G | G | A | C | G | T | C | C | G | C | G | A | C | T | G | A | |
| Ts18 (n = 1) | BEHZ | G | C | T | C | C | T | A | G | G | A | C | C | T | T | C | C | T | C | G | A | C | T | G | A |
| Ts19 (n = 1) | 795 | G | C | T | C | T | T | A | G | G | A | C | G | T | C | C | C | T | C | G | A | C | T | G | A |
| Ts20 (n = 2) | 754 & 793 | G | C | T | C | C | T | A | G | G | G | T | G | T | T | T | C | G | C | G | A | C | T | G | A |
| Ts21 (n = 1) | 411 | G | C | T | C | C | T | A | G | G | A | C | G | T | T | C | C | G | C | G | A | C | C | G | A |
| Ts22 (n = 1) | 737 | G | C | T | C | T | T | A | G | G | A | C | G | T | T | C | C | G | C | G | A | C | T | G | A |
| Ts23 (n = 1) | 735 | G | C | T | C | T | T | A | G | G | G | T | G | T | T | C | C | T | C | G | A | C | T | G | A |
| Ts24 (n = 1) | 801 | G | C | T | C | T | T | A | G | G | A | C | C | T | T | C | C | T | C | T | A | T | T | G | A |
| Ts25 (n = 1) | 707 | G | C | T | C | T | T | A | G | G | A | C | G | T | C | C | C | G | C | T | A | C | T | G | A |
| Ts26 (n = 1) | 772 | T | C | C | G | T | T | A | G | G | A | C | G | T | T | C | C | T | C | G | A | C | T | G | A |
| Ts27 (n = 1) | 710 | G | C | T | C | T | T | A | G | G | A | C | C | T | T | C | C | G | C | G | A | C | T | G | A |
| Ts28 (n = 1) | 37 | G | T | C | C | C | T | A | G | G | A | C | G | T | T | C | C | G | C | G | A | C | T | G | A |
| Ts29 (n = 1) | 908 | G | C | T | C | C | C | A | G | G | G | C | G | T | T | C | C | G | C | G | A | C | T | G | A |
| Ts30 (n = 1) | 788 | G | C | T | C | T | T | A | G | G | A | C | G | T | C | C | C | G | C | G | G | C | T | G | A |
| Ts31 (n = 1) | 780 | G | C | T | C | C | T | A | G | G | G | T | G | T | T | C | C | G | C | T | A | C | T | G | A |
| Ts32 (n = 1) | 778 | G | C | T | C | T | T | A | G | G | A | C | C | T | C | C | C | G | C | G | A | C | T | G | A |
| Ts33 (n = 1) | 771 | G | C | T | C | C | T | A | G | G | G | T | G | T | T | C | C | G | C | G | A | C | T | G | C |
Single nucleotide polymorphism (SNPs) in the alignment of the citrus nematode (Tylenchulus semipenetrans) COI mtDNA partial gene_
| COI haplotypes | Isolate(s) | Position of the single nucleotide variations/polymorphisms (SNVs/SNPs) on the sequences | |||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 49 | 250 | 353 | 385 | 393 | 406 | 425 | 448 | 469 | 481 | 515 | 532 | 535 | 562 | 574 | 580 | ||
| TsI (n = 21) | 771, 908, 32, 710, 801, 735, 737, 793, 795, 746, AMI, BEHZ, 25, 42, 112, 749, 759, ARE, 802, 25 & 812 | C | G | A | T | A | A | T | A | T | A | A | G | A | C | A | T |
| TsII (n = 10) | 698, 754, 707, 733, 716, 763, BEH, 712, 765 & 921 | T | A | A | C | A | A | T | A | A | A | A | A | A | C | A | T |
| TsIII (n = 11) | 720, 772, 773, 821, 743, 411, SH1, SH2, 785, 755 & 818 | T | A | A | T | A | A | T | T | A | G | A | A | G | T | G | T |
| TsIV (n = 1) | 740-2 | T | A | A | C | A | A | T | T | A | G | A | A | G | T | G | T |
| TsV (n = 1) | 789 | T | A | A | C | A | A | T | A | A | A | A | A | A | T | G | T |
| TsVI (n = 1) | 706 | C | G | A | T | A | A | T | A | T | A | G | G | A | C | A | T |
| TsVII (n = 1) | 780 | C | G | A | T | C | G | T | A | T | A | A | G | A | C | A | T |
| TsVIII (n = 1) | 778 | C | G | C | T | A | A | T | A | T | A | G | G | A | C | A | T |
| TsIX (n = 1) | 777 | C | G | A | T | A | A | T | A | T | A | A | G | A | C | A | G |
| TsX (n = 1) | 717 | C | G | A | T | A | A | G | A | T | A | A | G | A | C | A | T |
Morphometrics of males of 30 populations (five specimens each) of Tylenchulus semipenetrans, collected from citrus orchards of Fars Province, Iran_ Data are given as mean ± standard deviation (range) of population means or specimens_ Measurements are in μm_
| Characters | Means of populations | Specimens |
|---|---|---|
| n | 30 | 143 |
| L | 346 ± 13.2 (318.3–375) | 347 ± 21 (296–424) |
| a | 34.9 ± 1.9 (31.7–39.7) | 34.9 ± 2.8 (29.6–44.9) |
| b | 3.4 ± 0.2 (3.1–3.8) | 3.5 ± 0.2 (2.8–4.3) |
| c | 9.7 ± 0.5 (8.5–10.8) | 9.7 ± 0.7 (8.0–12.1) |
| c’ | 4.7 ± 0.3 (4.1–5.5) | 4.7 ± 0.5 (3.5–6.1) |
| Stylet | 8.4 ± 0.4 (7.7–9.2) | 8.4 ± 0.6 (7–11.1)a |
| Conus | 4.9 ± 0.3 (4.3–5.7) | 4.9 ± 0.5 (3.5–6.4) |
| Anterior end to the center of the median bulb | 43.7 ± 3.2 (34.2–50) | 43.7 ± 4.6 (31–64) |
| Pharynx length | 100 ± 4.8 (90–111) | 101 ± 7.6 (83–123) |
| Anterior end to hemizonid | 65.6 ± 2.8 (58.6–70.6) | 65.6 ± 4.1 (52–77) |
| Anterior end to nerve ring | 61.1 ± 3.0 (53.0–66.7) | 61.1 ± 4.2 (48.5–72.5) |
| Secretory-excretory pore (S. E. pore) to cloaca | 100 ± 6.1 (87–110) | 101 ± 8.9 (75–119.5) |
| Anterior end to S. E. pore | 231 ± 20.7 (198–279) | 229 ± 44 (161–333) |
| S. E. pore from the anterior end (% of L) | 66.9 ± 5.3 (58.0–78.) | 66 ± 12 (54–91.2) |
| Median bulb width | 4.5 ± 0.6 (3.2–5.6) | 4.5 ± 0.7 (2.7–6.2) |
| Median bulb length | 11.2 ± 0.9 (9.3–13.0) | 11.2 ± 1.4 (7.1–14) |
| Median bulb length/diam. Ratio | 252 ± 30.3 (199–328) | 251 ± 41 (176–385) |
| Max. body width (BW) | 10.0 ± 0.5 (9.1–11.0) | 10.0 ± 0.8 (8.3–13) |
| BW in Pharynx | 8.8 ± 0.4 (8.0–9.6) | 8.8 ± 0.6 (7.3–10.7) |
| Anal BW | 7.7 ± 0.5 (7.3–10.2) | 7.7 ± 0.9 (6.7–16.4) |
| Spicules (curved median line) | 16.6 ± 0.7 (15.5–17.8) | 16.6 ± 1.1 (14–20) |
| Gubernaculum | 3.7 ± 0.3 (3.1–4.3) | 3.7 ± 0.5 (2.5–5.0) |
| Tail | 36.0 ± 2.4 (33.1–42.0) | 36 ± 3.4 (29–47.5) |
| M | 0.6 ± 0.0 (0.5–0.6) | 60 ± 6 (41–77) |
| Stylet / L (%) | 2.4 ± 0.1 (2.2–2.6) | 2.4 ± 0.2 (2.1–3.2) |
| S. E. pore / L (%) | 66.9 ± 5.3 (58.0–78.7) | 66 ± 12 (53.9–91.2) |