Have a personal or library account? Click to login

Description and Molecular Characterization of a New Dorylaimid Nematode, Mesodorylaimus pini n. sp. (Nematoda: Dorylaimidae) from Korea

Open Access
|Sep 2024

Figures & Tables

Figure 1.

Illustrations of Mesodorylaimus pini n. sp. (A-L): A: Female whole body; B: Male whole body; C: Female anterior region; D: Female rectum and prerectum region; E: The expanded part of pharynx; F: Junction between intestine and prerectum with a long conical or tongue-like structure; G: Posterior end of male, including copulatory apparatus and the arrangement of ventromedian supplements; H, I: Vulval region, with variation in shape of sclerotizations; J: Female tail region; K: Female reproductive system; L: Sperm cells in testis and uterus.
Illustrations of Mesodorylaimus pini n. sp. (A-L): A: Female whole body; B: Male whole body; C: Female anterior region; D: Female rectum and prerectum region; E: The expanded part of pharynx; F: Junction between intestine and prerectum with a long conical or tongue-like structure; G: Posterior end of male, including copulatory apparatus and the arrangement of ventromedian supplements; H, I: Vulval region, with variation in shape of sclerotizations; J: Female tail region; K: Female reproductive system; L: Sperm cells in testis and uterus.

Figure 2.

Photomicrographs of Mesodorylaimus pini n. sp. (A-J). A, B: Variation in female anterior region; C: Junction between intestine and prerectum with a long conical or tongue-like structure; D, E, F: Variation in female tail region; G: Male tail region; H: Male posterior region with ventromedian supplements and copulatory apparatus; I, J: Vulval region and variation in shape of sclerotizations (Scale bars: A, B, C, E, F, G, I and J = 10 μm; D and H = 20 μm).
Photomicrographs of Mesodorylaimus pini n. sp. (A-J). A, B: Variation in female anterior region; C: Junction between intestine and prerectum with a long conical or tongue-like structure; D, E, F: Variation in female tail region; G: Male tail region; H: Male posterior region with ventromedian supplements and copulatory apparatus; I, J: Vulval region and variation in shape of sclerotizations (Scale bars: A, B, C, E, F, G, I and J = 10 μm; D and H = 20 μm).

Figure 3.

Bayesian tree inferred under the GTR + I + G model from 18S-rRNA gene sequences of Dorylaimid species. Posterior probability values exceeding 50% are given on appropriate clades. The studied population is indicated in bold text. Outgroup taxa: Paravulvus hartingii and Nygolaimus cf. parvus.
Bayesian tree inferred under the GTR + I + G model from 18S-rRNA gene sequences of Dorylaimid species. Posterior probability values exceeding 50% are given on appropriate clades. The studied population is indicated in bold text. Outgroup taxa: Paravulvus hartingii and Nygolaimus cf. parvus.

Figure 4.

Bayesian tree inferred under the GTR + I + G model from LSU D2–D3 partial sequences of Dorylaimid species. Posterior probability values exceeding 50% are given on appropriate clades. The studied population is indicated in bold text. Outgroup taxa: Mononchus tunbridgensis and Anatonchus tridentatus.
Bayesian tree inferred under the GTR + I + G model from LSU D2–D3 partial sequences of Dorylaimid species. Posterior probability values exceeding 50% are given on appropriate clades. The studied population is indicated in bold text. Outgroup taxa: Mononchus tunbridgensis and Anatonchus tridentatus.

Morphometrics of Mesodorylaimus pini n_ sp_ from Korea

CharacteristicHolotype♀♀♂♂

n 3132
L18021702.6 ±101.5 (1503–1889)1558.2 ± 103.0 (1366–1775)
a44.543.0 ± 2.2 (37.7–47.0)40.6 ± 2.2 (35.3–44.9)
b4.84.6 ± 0.3 (4.0–5.1)4.3 ± 0.2 (3.6–4.8)
c10.611.9 ± 1.0 (10.0–13.6)73.1 ± 8.1 (56.3–87.5)
c’6.55.8 ± 0.6 (4.9–7.0)0.8 ± 0.1 (0.6–0.8)
V50.551.4 ± 1.2 (50.0–54.9)_
G1%17.417.8 ± 1.7 (14.3–19.6)_
G2%17.818.6 ± 1.8 (14.6–20.7)_
Lip height5.05.0 ± 0.4 (4.5–6.0)5.2 ± 0.5 (4.5–6.0)
Lip diam.12.512.2 ± 0.5 (11.0–13.5)12.3 ± 0.5 (11.0–13.0)
Anterior to guiding ring9.09.2 ± 0.3 (8.5–9.5)9.2 ± 0.2 (9.0–9.5)
Odontostyle18.018.2 ± 1.0 (17.0–19.0)18.1 ± 0.9 (17.0–19.0)
Odontophore21.522.0 ± 1.2 (19.0–24.0)21.7 ± 1.5 (19.0–23.5)
Total spear39.540.2 ±1.7 (36.0–42.5)39.9 ±1.9 (36.0–42.0)
Anterior to nerve ring127.0126.5 ± 5.2 (119.0–143.0)124.4 ± 2.7 (118.0–132.0)
Pharynx length377369.2 ± 16.7 (333–398)358.7 ± 16.7 (326–390)
Expanded part of pharynx160161.3 ± 9.6 (142.0–186.0)151.2 ± 11.1 (133.0–179.5)
Cardia length21.020.1 ± 3.6 (14.5–27.5)20.8 ± 3.3 (14.0–25.5)
Maximum body diam.40.539.7 ± 2.2 (32.0–43.5)38.4 ± 2.1 (33.0–42.0)
Vulva to anus739729.2 ± 35.3 (647–817)_
Prerectum78.580.8 ± 6.5 (69.0–99.0)119.2 ± 9.1 (110.5–151.5)
Rectum31.532.0 ± 2.6 (28.0–38.0)44.0 ± 4.0 (35.0–49.5)
Anal / cloacal body diam.26.024.7±1.3 (22.0–26.5)28.5 ± 1.6 (25.0–32.0)
Tail length179.0144.4 ± 18.4 (115.0–187.0)21.5 ± 1.8 (18.0–25.0)
Spicules__52.3 ± 1.8 (48.0–57.0)
No. of ventromedian supplements__7–8
DOI: https://doi.org/10.2478/jofnem-2024-0028 | Journal eISSN: 2640-396X | Journal ISSN: 0022-300X
Language: English
Submitted on: Mar 30, 2024
Published on: Sep 30, 2024
Published by: Society of Nematologists, Inc.
In partnership with: Paradigm Publishing Services
Publication frequency: 1 issue per year

© 2024 Abraham Okki Mwamula, Sang Myeong Lee, Young Hak Jung, Yi Seul Kim, Dong Woon Lee, published by Society of Nematologists, Inc.
This work is licensed under the Creative Commons Attribution 4.0 License.