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Description of Nothotylenchus savadkoohensis n. sp. (Rhabditida, Anguinidae) from Iran based on morphological and molecular data Cover

Description of Nothotylenchus savadkoohensis n. sp. (Rhabditida, Anguinidae) from Iran based on morphological and molecular data

Open Access
|Apr 2024

Figures & Tables

Figure 1:

Line drawings of Nothotylenchus savadkoohensis n. sp. A, B: Male and female entire body; C: Anterior body region; D: Female pharyngeal region; E: Male posterior body region; F: Female posterior body region.
Line drawings of Nothotylenchus savadkoohensis n. sp. A, B: Male and female entire body; C: Anterior body region; D: Female pharyngeal region; E: Male posterior body region; F: Female posterior body region.

Figure 2:

Light micrographs of Nothotylenchus savadkoohensis n. sp. A: Anterior body region; B & C: Non-muscular metacorpus; D: Vulva region and postvulval uterine sac; E: Female posterior body region (tail); F: Female tail tip; G: Male posterior body region and bursa; H & I: Short overlapping of pharyngeal bulb; J: Lateral field; K: Spicules (Scale bars: A-I, K=10 μm, J=5 μm).
Light micrographs of Nothotylenchus savadkoohensis n. sp. A: Anterior body region; B & C: Non-muscular metacorpus; D: Vulva region and postvulval uterine sac; E: Female posterior body region (tail); F: Female tail tip; G: Male posterior body region and bursa; H & I: Short overlapping of pharyngeal bulb; J: Lateral field; K: Spicules (Scale bars: A-I, K=10 μm, J=5 μm).

Figure 3:

Bayesian 50% majority rule consensus tree inferred from D2–D3 expansion region of LSU rDNA sequence of Nothotylenchus savadkoohensis n. sp. from Mazandaran province under the GTR + G + I model. Bayesian posterior probability (BPP) values >0.50 are given for appropriate clades. The newly generated sequence of the new species is in bold font.
Bayesian 50% majority rule consensus tree inferred from D2–D3 expansion region of LSU rDNA sequence of Nothotylenchus savadkoohensis n. sp. from Mazandaran province under the GTR + G + I model. Bayesian posterior probability (BPP) values >0.50 are given for appropriate clades. The newly generated sequence of the new species is in bold font.

Figure 4:

Bayesian 50% majority rule consensus tree inferred from ITS rDNA sequence of Nothotylenchus savadkoohensis n. sp. from Mazandaran province under the HKY + G model. Bayesian posterior probability (BPP) values >0.50 are given for appropriate clades. The newly generated sequence of the new species is in bold font.
Bayesian 50% majority rule consensus tree inferred from ITS rDNA sequence of Nothotylenchus savadkoohensis n. sp. from Mazandaran province under the HKY + G model. Bayesian posterior probability (BPP) values >0.50 are given for appropriate clades. The newly generated sequence of the new species is in bold font.

Morphometrics of Nothotylenchus savadkoohensis n_ sp_ All measurements are in μm and in the form: mean ± standard deviation (range)_

CharacterFemalesMales

HolotypeParatypesParatypes
n-124
L485526±80 (379–662)496.3±8.5 (485–505)
a37.338.4±5.2 (27.1–47.3)37.9±2.4 (34.6–40.4)
b4.85.4±0.7 (4.2–6.3)5.2±0.2 (5.0–5.4)
c9.711.1±1.4 (8.4–13.9)10.1±0.9 (9.2–11.2)
c′6.76.1±0.6 (4.9–7.3)6.3±0.9 (5.0–6.9)
V79.481.8±2.3 (76.5–84.0)-
Anterior end to vulva385426±74 (290–550)-
Lip region height32.5±0.5 (2.0–3.2)2.3±0.2 (2.0–2.5)
Lip region width55.0±0.3 (4.8–5.7)5.0±0.1 (4.8–5.1)
Stylet conus22.1±0.2 (2.0–2.5)2.2±0.1 (2.0–2.3)
Stylet66.2±0.4 (5.8–6.9)6.21±0.4 (5.6–6.5)
m33.333.8±2.2 (30.8–37.1)34.9±1.9 (32.3–36.5)
Anal/cloacal diameter7.58.5±0.5 (8–9)8.0±0.8 (7.2–9.0)
Maximum body with1313.6±0.9 (12–15)13.1±0.6 (12.5–14.0)
Vulval body width1111.9±1.2 (10–14)-
Anal body width7.57.9±3.4 (7.5–9.07.7±0.6 (7–8)
Excretory pore7776.3±3.4 (68–80)72.7±1.8 (71.0–74.5)
Hemizonid7170.0±1.5 (67–72)70.7±2.1 (68.0–72.6)
Pharynx10297.6±5.6 (90–105)96.5±5.2 (90–102)
Nerve ring5556.6±2.5 (53–62)60±5 (53–65)
Overlap length4.94.8±0.2 (4.5–5.2)3.0±0.7 (4.0–4.8)
Postvulval uterine sac (PUS)1518.7±3.1 (15–26)-
Vulva to anus4545.4±5.7 (40.0–55.5)-
Tail5047.8±4.4 (42–55)49.5±3.3 (45–53)
Spicules--13.5±0.4 (13.0–14.5)
Gubernaculum--4.8±0.2 (4.5–5.0)
DOI: https://doi.org/10.2478/jofnem-2024-0015 | Journal eISSN: 2640-396X | Journal ISSN: 0022-300X
Language: English
Submitted on: Jan 22, 2024
Published on: Apr 22, 2024
Published by: Society of Nematologists, Inc.
In partnership with: Paradigm Publishing Services
Publication frequency: 1 issue per year

© 2024 Soheila Rezaei, Ebrahim Pourjam, Mohammad Reza Atighi, Majid Pedram, published by Society of Nematologists, Inc.
This work is licensed under the Creative Commons Attribution 4.0 License.