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Description of Nothotylenchus savadkoohensis n. sp. (Rhabditida, Anguinidae) from Iran based on morphological and molecular data

Open Access
|Apr 2024

Figures & Tables

Figure 1:

Line drawings of Nothotylenchus savadkoohensis n. sp. A, B: Male and female entire body; C: Anterior body region; D: Female pharyngeal region; E: Male posterior body region; F: Female posterior body region.
Line drawings of Nothotylenchus savadkoohensis n. sp. A, B: Male and female entire body; C: Anterior body region; D: Female pharyngeal region; E: Male posterior body region; F: Female posterior body region.

Figure 2:

Light micrographs of Nothotylenchus savadkoohensis n. sp. A: Anterior body region; B & C: Non-muscular metacorpus; D: Vulva region and postvulval uterine sac; E: Female posterior body region (tail); F: Female tail tip; G: Male posterior body region and bursa; H & I: Short overlapping of pharyngeal bulb; J: Lateral field; K: Spicules (Scale bars: A-I, K=10 μm, J=5 μm).
Light micrographs of Nothotylenchus savadkoohensis n. sp. A: Anterior body region; B & C: Non-muscular metacorpus; D: Vulva region and postvulval uterine sac; E: Female posterior body region (tail); F: Female tail tip; G: Male posterior body region and bursa; H & I: Short overlapping of pharyngeal bulb; J: Lateral field; K: Spicules (Scale bars: A-I, K=10 μm, J=5 μm).

Figure 3:

Bayesian 50% majority rule consensus tree inferred from D2–D3 expansion region of LSU rDNA sequence of Nothotylenchus savadkoohensis n. sp. from Mazandaran province under the GTR + G + I model. Bayesian posterior probability (BPP) values >0.50 are given for appropriate clades. The newly generated sequence of the new species is in bold font.
Bayesian 50% majority rule consensus tree inferred from D2–D3 expansion region of LSU rDNA sequence of Nothotylenchus savadkoohensis n. sp. from Mazandaran province under the GTR + G + I model. Bayesian posterior probability (BPP) values >0.50 are given for appropriate clades. The newly generated sequence of the new species is in bold font.

Figure 4:

Bayesian 50% majority rule consensus tree inferred from ITS rDNA sequence of Nothotylenchus savadkoohensis n. sp. from Mazandaran province under the HKY + G model. Bayesian posterior probability (BPP) values >0.50 are given for appropriate clades. The newly generated sequence of the new species is in bold font.
Bayesian 50% majority rule consensus tree inferred from ITS rDNA sequence of Nothotylenchus savadkoohensis n. sp. from Mazandaran province under the HKY + G model. Bayesian posterior probability (BPP) values >0.50 are given for appropriate clades. The newly generated sequence of the new species is in bold font.

Morphometrics of Nothotylenchus savadkoohensis n_ sp_ All measurements are in μm and in the form: mean ± standard deviation (range)_

CharacterFemalesMales

HolotypeParatypesParatypes
n-124
L485526±80 (379–662)496.3±8.5 (485–505)
a37.338.4±5.2 (27.1–47.3)37.9±2.4 (34.6–40.4)
b4.85.4±0.7 (4.2–6.3)5.2±0.2 (5.0–5.4)
c9.711.1±1.4 (8.4–13.9)10.1±0.9 (9.2–11.2)
c′6.76.1±0.6 (4.9–7.3)6.3±0.9 (5.0–6.9)
V79.481.8±2.3 (76.5–84.0)-
Anterior end to vulva385426±74 (290–550)-
Lip region height32.5±0.5 (2.0–3.2)2.3±0.2 (2.0–2.5)
Lip region width55.0±0.3 (4.8–5.7)5.0±0.1 (4.8–5.1)
Stylet conus22.1±0.2 (2.0–2.5)2.2±0.1 (2.0–2.3)
Stylet66.2±0.4 (5.8–6.9)6.21±0.4 (5.6–6.5)
m33.333.8±2.2 (30.8–37.1)34.9±1.9 (32.3–36.5)
Anal/cloacal diameter7.58.5±0.5 (8–9)8.0±0.8 (7.2–9.0)
Maximum body with1313.6±0.9 (12–15)13.1±0.6 (12.5–14.0)
Vulval body width1111.9±1.2 (10–14)-
Anal body width7.57.9±3.4 (7.5–9.07.7±0.6 (7–8)
Excretory pore7776.3±3.4 (68–80)72.7±1.8 (71.0–74.5)
Hemizonid7170.0±1.5 (67–72)70.7±2.1 (68.0–72.6)
Pharynx10297.6±5.6 (90–105)96.5±5.2 (90–102)
Nerve ring5556.6±2.5 (53–62)60±5 (53–65)
Overlap length4.94.8±0.2 (4.5–5.2)3.0±0.7 (4.0–4.8)
Postvulval uterine sac (PUS)1518.7±3.1 (15–26)-
Vulva to anus4545.4±5.7 (40.0–55.5)-
Tail5047.8±4.4 (42–55)49.5±3.3 (45–53)
Spicules--13.5±0.4 (13.0–14.5)
Gubernaculum--4.8±0.2 (4.5–5.0)
DOI: https://doi.org/10.2478/jofnem-2024-0015 | Journal eISSN: 2640-396X | Journal ISSN: 0022-300X
Language: English
Submitted on: Jan 22, 2024
Published on: Apr 22, 2024
Published by: Society of Nematologists, Inc.
In partnership with: Paradigm Publishing Services
Publication frequency: 1 times per year

© 2024 Soheila Rezaei, Ebrahim Pourjam, Mohammad Reza Atighi, Majid Pedram, published by Society of Nematologists, Inc.
This work is licensed under the Creative Commons Attribution 4.0 License.