Vertical Migration of Second-stage Juveniles of Meloidogyne enterolobii as Influenced by Temperature and Host

Ana Karina S. Oliveira; Elvira M. R. Pedrosa; Diego A. H. S. Leitão; Janete A. Brito; Ênio F. de F. Silva; Donald W. Dickson

doi:10.2478/jofnem-2024-0012

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Vertical Migration of Second-stage Juveniles of Meloidogyne enterolobii as Influenced by Temperature and Host

Journal of Nematology

Volume 56 (2024): Issue 1 (March 2024)

By: Ana Karina S. Oliveira, Elvira M. R. Pedrosa, Diego A. H. S. Leitão, Janete A. Brito, Ênio F. de F. Silva and Donald W. Dickson

Open Access

|Apr 2024

Figures & Tables

Diagram sketch of an experimental soil-filled column. Each column was comprised of PVC pipe cut into three 4-cm-long sections. Each section had an internal diameter of 4.4 cm and was taped tightly together. A 2-cm-long injection ring was placed at the bottom of each migration column with a hole through which nematode suspensions could be injected. A 15-µm mesh screen was attached to the bottom of each injection ring. A 300-cm3 Styrofoam cup with the bottom removed was taped to the top of each migration column. Each cup had a nylon mesh screen with 35-µm openings taped to the bottom. One-third of the columns had no plant, one-third contained tomato (Solanum lycopersicum), and one-third contained marigold (Tagetes patula) seedlings.

Experiment step-by-step. A: Soil columns filled with sandy soil; B: Styrofoam cups with either no plant, tomato, or marigold were attached to the columns and then transferred to separate growth chambers, each set at either 20 or 26ºC; C: Injection of suspension of second-stage juveniles of Meloidogyne enterolobii into the hole in the injection ring; D: Dismantling of each column was performed with a spatula to separate the rings; E: The 35-µm mesh screen prevented roots from growing into the columns; F: An example of a tomato plant root system at 12 DAI; G: Soil from each ring was placed in separate cups for centrifugal-flotation extraction of second-stage juveniles; H: Root staining with acid fuchsin was used for determining nematode penetration numbers.

Distribution of recovered second-stage juveniles (J2) of Meloidogyne enterolobii extracted from PVC column sections each 4-cm long × 4.4-cm internal diameter. A. The percentages of second-stage juveniles (J2) migrating in columns containing either tomato, marigold, or no plant untreated control, where bars represent average data pooled from all temperatures and days after inoculation (DAI) (n = 32). B. The percentages of J2 migrating in columns sampled at four dates 3, 6, 9, and 12 DAI, where bars represent average data pooled from all temperatures and stimuli (n = 24). The bars among the different sampling periods are statistically different according to X2 test (P < 0.01).

Distribution of active second-stage juveniles (J2) of Meloidogyne enterolobii in PVC soil columns (sections were 4-cm long and 4.4-cm internal diameter) over distance migrated (cm) and sampling time (3, 6, 9, and 12 days after inoculation [DAI]) at two different temperatures: 20ºC (A) and 26ºC (B). Each bar represents the average data pooled from all plant stimuli (n = 12). Distribution percentages were statistically different according to X2 test (P < 0.01).

Penetration of second-stage juveniles (J2) of Meloidogyne enterolobii into tomato and marigold roots (A) and their rates over time for both plant species (B). Bars represent means of J2 compared by LSD test. Different letters indicate statistically different penetration rates at 5% of probability.

Penetration of second-stage juveniles (J2) of Meloidogyne enterolobii into tomato and marigold roots across 3, 6, 9, and 12 days after inoculation (DAI). Bars represent the average of J2 per plant for both temperatures (n=8), and error bars represent the standard errors of the mean. There was no interaction between stimulus and time; therefore, no statistical analysis was performed for this data.

Repeated measure MANOVA summary of second-stage juveniles (J2) of Meloidogyne enterolobii inside roots after migrating through sandy soil-filled PVC columns_

Source	df	J2 inside roots

		SS	MS	F	p-value
Block	3	2.96	0.99	2.99	0.0409
Temperature (Temp)	1	0.02	0.02	0.05	0.8333
Stimulus (Stim)	1	7.48	7.48	22.69	<0.0001
Temp×Stim	1	0.02	0.02	0.06	0.8067
Time	3	9.01	3.00	9.10	<0.0001
Time×Temp	3	0.22	0.07	0.22	0.8841
Time×Stim	3	2.74	0.91	2.76	0.0528
Time×Temp×Stim	3	0.07	0.02	0.07	0.9748

Repeated measure MANOVA summary of the effects of temperature, plant stimulus, section, and time on second-stage juveniles (J2) of Meloidogyne enterolobii vertical migration in PVC columns filled with sandy soil_

Source	Recovered J2					Active J2

	df	SS	MS	F	p-value	SS	MS	F	p-value
Block	3	16.00	5.50	1.04	0.3755	86.91	28.97	8.37	<0.0001
Temperature (Temp)	1	129.20	129.20	24.62	<0.0001	194.18	194.18	56.13	<0.0001
Stimulus (Stim)	2	78.40	39.20	7.47	0.0007	47.94	23.97	6.93	0.0011
Section (Sec)	4	3884.40	971.10	184.97	<0.0001	628.20	157.05	45.40	<0.0001
Temp×Stim	2	10.80	5.40	1.02	0.3603	1.30	0.65	0.19	0.8276
Temp×Sec	4	4.40	1.10	0.20	0.9380	8.60	2.15	0.62	0.6469
Stim×Sec	8	96.00	12.00	2.29	0.0210	41.12	5.14	1.48	0.1614
Temp×Stim×Sec	8	78.40	9.80	1.86	0.0653	35.44	4.43	1.28	0.2526
Time	3	142.20	47.40	9.03	<0.0001	146.28	48.76	14.09	<0.0001
Time×Temp	3	3.00	1.00	0.19	0.9065	16.98	5.66	1.64	0.1805
Time×Stim	6	45.00	7.50	1.43	0.2021	15.00	2.50	0.72	0.6322
Time×Sec	12	730.80	60.90	11.60	<0.0001	493.68	41.14	11.89	<0.0001
Time×Temp×Stim	6	22.80	3.80	0.71	0.6383	15.72	2.62	0.76	0.6034
Time×Temp×Sec	12	55.20	4.60	0.88	0.5640	82.20	6.85	2.01	0.0228
Time×Stim×Sec	24	193.00	8.04	1.53	0.0547	106.80	4.45	1.29	0.1682
Time×Temp×Stim×Sec	24	64.80	2.70	0.52	0.9711	44.64	1.86	0.54	0.9652

Chemical attributes characterization of the soil used to fill the columns_

Chemical attributes	Unit	Depth (m) 0.00–0.40
pH	(1:2.5)	5.6
Extractable P	mg Kg⁻¹	>135
Extractable K	mg Kg⁻¹	13
Extractable Mg	mg Kg⁻¹	0
Extractable Ca	mg Kg⁻¹	164
Organic Matter	%	0.27

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DOI: https://doi.org/10.2478/jofnem-2024-0012 | Journal eISSN: 2640-396X | Journal ISSN: 0022-300X

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Language: English

Submitted on: Nov 1, 2023

Published on: Apr 22, 2024

Published by: Society of Nematologists, Inc.

In partnership with: Paradigm Publishing Services

Publication frequency: 1 issue per year

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© 2024 Ana Karina S. Oliveira, Elvira M. R. Pedrosa, Diego A. H. S. Leitão, Janete A. Brito, Ênio F. de F. Silva, Donald W. Dickson, published by Society of Nematologists, Inc.
This work is licensed under the Creative Commons Attribution 4.0 License.

Volume 56 (2024): Issue 1 (March 2024)

Vertical Migration of Second-stage Juveniles of Meloidogyne enterolobii as Influenced by Temperature and Host

Figures & Tables

Figure 1:

Figure 2:

Figure 3:

Figure 4:

Figure 5:

Supplementary Figure 1:

Repeated measure MANOVA summary of second-stage juveniles (J2) of Meloidogyne enterolobii inside roots after migrating through sandy soil-filled PVC columns_

Repeated measure MANOVA summary of the effects of temperature, plant stimulus, section, and time on second-stage juveniles (J2) of Meloidogyne enterolobii vertical migration in PVC columns filled with sandy soil_

Chemical attributes characterization of the soil used to fill the columns_

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