Figure 1:
Line drawings of Paratylenchus vandenbrandei De Grisse, 1962. A, E, J: Male. A: Entire body; E: Anterior body region; J: Posterior body region; B-D, F-I: Female. B: Entire body; C, D: Anterior body region; F: Lateral field at mid-body; G-I: Posterior body region.
Figure 2:
Light photomicrographs of Paratylenchus vandenbrandei De Grisse, 1962. A, C-E, I-O: Female. A: Entire body; C-E: Anterior body region; I: Lateral field at mid-body; J, K: Reproductive system (the arrow indicates the spermatheca); L-O: Posterior body region (the arrow indicates the advulval flap); B, F-H, P, Q: Male. B: Entire body; F-H: Anterior body region; P, Q: Posterior body region. (Scale bars: A, B = 20 μm; C-Q = 10 μm).
Figure 3:
Light photomicrographs of Paratylenchus vandenbrandei De Grisse, 1962. Juvenile. A: Entire body; B, C: Anterior body region (the arrow indicates the excretory pore); D, E: Posterior body region. (Scale bars: A = 20 μm; B-E = 10 μm).
Table 1.
Morphometrics of Paratylenchus vandenbrandei from Khuzestan province, Iran, and comparison with the original description. All measurements are in μm and in the form: mean ± s.d. (range).
| Character | Present study | De Grisse, 1962 | ||
|---|---|---|---|---|
| Female | Male | Female | Male | |
| n | 11 | 3 | 19 | 3 |
| L | 296 ±12 (259–314) | 308 ± 53 (275–369) | 269.0 ± 3.6 (248–290) | 261 (248–269) |
| a | 22.4 ± 0.8 (21.3–23.2) | 29.6 ± 3.3 (27.2–32.0) | 20 (14–24) | 28 (27–30) |
| b | 3.9 ± 0.2 (3.7–4.5) | 4.4 ± 0.6 (4.2–4.6) | 3.5 (3.2–3.7) | - |
| c | 20.0 ± 2.3 (15–22) | 19.6 ± 1.9 (18.3–21.0) | 18 (15–21) | 16.6 (16.5–16.8) |
| c′ | 2.0 ± 0.2 (1.6–2.3) | 1.7 ± 0.07 (1.7–1.8) | 2–3 | - |
| V | 80.0 ± 2.1 (79.0–84.5) | - | 82 (81–86) | - |
| T | - | 19.2 ± 2.7 (17.3–21.2) | - | - |
| Stylet length | 27 ± 1.7 (24.0–28.8) | - | 31.0 ± 5.6 (28–33) | - |
| Conus length | 17.1 ± 2.5 (14.7–19.5) | - | - | - |
| m | 71.1 ± 1.5 (65.1–74.2) | - | - | - |
| DGO | 3.5 ± 0.5 (3.2–4.2) | - | - | |
| Anterior end to excretory pore | 63.4 ± 3.0 (58–67) | 61.0 ± 8.4 (55–67) | 59–68 | 58–64 |
| Pharynx length | 74.6 ± 5.9 (68.5–81.2) | 72.0 ± 9.8 (65–79) | - | - |
| Maximum body width | 12.9 ± 0.8 (12.0–14.0) | 10.9 ± 3.1 (10.1–12.5) | - | - |
| Vulva body width | 12.2 ± 1.0 (11.5–13.5) | - | - | - |
| Anal body width | 7.5 ± 2.0 (6.5–9.2) | 9.1 ± 0.8 (8.5–9.7) | - | - |
| Head-vulva | 239.5 ± 8.5 (228–248) | - | - | - |
| Head-anus | 275.2 ±10.1 (264–292) | - | - | - |
| Vulva-anus | 39.5 ± 6.5 (29–48) | - | - | - |
| St/L% | 11.4 ± 0.7 (9.5–12.3) | - | - | - |
| Tail length | 15.1 ± 1.4 (14.0–17.5) | 16.1 ± 1.5 (15.0–17.2) | 15 (12–18) | 15.6 (15–16) |
| Spicule length | - | 16.2 ± 1.7 (15.5–17.5) | - | 14.5 (14–15) |
| Gubernaculum length | - | 3.5 ± 1.7 (3.0–4.0) | - | 2.5–3.0 |
Figure 4:
Bayesian 50% majority rule consensus tree inferred from analysis of the D2-D3 domains of the LSU rDNA sequences of Paratylenchus vandenbrandei De Grisse, 1962. under the GTR + G + I model. Bayesian posterior probability values of more than 0.50 are given for appropriate clades. New sequences are indicated in bold.
Figure 5:
Bayesian 50% majority rule consensus tree inferred from analysis of the ITS rDNA of Paratylenchus vandenbrandei De Grisse, 1962. under the GTR + G + I model. Bayesian posterior probability values of more than 0.50 are given for appropriate clades. New sequences are indicated in bold.