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First Morphological and Molecular Characterization of Paratylenchus vandenbrandei (Rhabditida: Tylenchulidae) in Iran

Open Access
|Dec 2023

Figures & Tables

Figure 1:

Line drawings of Paratylenchus vandenbrandei De Grisse, 1962. A, E, J: Male. A: Entire body; E: Anterior body region; J: Posterior body region; B-D, F-I: Female. B: Entire body; C, D: Anterior body region; F: Lateral field at mid-body; G-I: Posterior body region.
Line drawings of Paratylenchus vandenbrandei De Grisse, 1962. A, E, J: Male. A: Entire body; E: Anterior body region; J: Posterior body region; B-D, F-I: Female. B: Entire body; C, D: Anterior body region; F: Lateral field at mid-body; G-I: Posterior body region.

Figure 2:

Light photomicrographs of Paratylenchus vandenbrandei De Grisse, 1962. A, C-E, I-O: Female. A: Entire body; C-E: Anterior body region; I: Lateral field at mid-body; J, K: Reproductive system (the arrow indicates the spermatheca); L-O: Posterior body region (the arrow indicates the advulval flap); B, F-H, P, Q: Male. B: Entire body; F-H: Anterior body region; P, Q: Posterior body region. (Scale bars: A, B = 20 μm; C-Q = 10 μm).
Light photomicrographs of Paratylenchus vandenbrandei De Grisse, 1962. A, C-E, I-O: Female. A: Entire body; C-E: Anterior body region; I: Lateral field at mid-body; J, K: Reproductive system (the arrow indicates the spermatheca); L-O: Posterior body region (the arrow indicates the advulval flap); B, F-H, P, Q: Male. B: Entire body; F-H: Anterior body region; P, Q: Posterior body region. (Scale bars: A, B = 20 μm; C-Q = 10 μm).

Figure 3:

Light photomicrographs of Paratylenchus vandenbrandei De Grisse, 1962. Juvenile. A: Entire body; B, C: Anterior body region (the arrow indicates the excretory pore); D, E: Posterior body region. (Scale bars: A = 20 μm; B-E = 10 μm).
Light photomicrographs of Paratylenchus vandenbrandei De Grisse, 1962. Juvenile. A: Entire body; B, C: Anterior body region (the arrow indicates the excretory pore); D, E: Posterior body region. (Scale bars: A = 20 μm; B-E = 10 μm).

Figure 4:

Bayesian 50% majority rule consensus tree inferred from analysis of the D2-D3 domains of the LSU rDNA sequences of Paratylenchus vandenbrandei De Grisse, 1962. under the GTR + G + I model. Bayesian posterior probability values of more than 0.50 are given for appropriate clades. New sequences are indicated in bold.
Bayesian 50% majority rule consensus tree inferred from analysis of the D2-D3 domains of the LSU rDNA sequences of Paratylenchus vandenbrandei De Grisse, 1962. under the GTR + G + I model. Bayesian posterior probability values of more than 0.50 are given for appropriate clades. New sequences are indicated in bold.

Figure 5:

Bayesian 50% majority rule consensus tree inferred from analysis of the ITS rDNA of Paratylenchus vandenbrandei De Grisse, 1962. under the GTR + G + I model. Bayesian posterior probability values of more than 0.50 are given for appropriate clades. New sequences are indicated in bold.
Bayesian 50% majority rule consensus tree inferred from analysis of the ITS rDNA of Paratylenchus vandenbrandei De Grisse, 1962. under the GTR + G + I model. Bayesian posterior probability values of more than 0.50 are given for appropriate clades. New sequences are indicated in bold.

Morphometrics of Paratylenchus vandenbrandei from Khuzestan province, Iran, and comparison with the original description_ All measurements are in μm and in the form: mean ± s_d_ (range)_

CharacterPresent studyDe Grisse, 1962


FemaleMaleFemaleMale
n113193
L296 ±12 (259–314)308 ± 53 (275–369)269.0 ± 3.6 (248–290)261 (248–269)
a22.4 ± 0.8 (21.3–23.2)29.6 ± 3.3 (27.2–32.0)20 (14–24)28 (27–30)
b3.9 ± 0.2 (3.7–4.5)4.4 ± 0.6 (4.2–4.6)3.5 (3.2–3.7)-
c20.0 ± 2.3 (15–22)19.6 ± 1.9 (18.3–21.0)18 (15–21)16.6 (16.5–16.8)
c′2.0 ± 0.2 (1.6–2.3)1.7 ± 0.07 (1.7–1.8)2–3-
V80.0 ± 2.1 (79.0–84.5)-82 (81–86)-
T-19.2 ± 2.7 (17.3–21.2)--
Stylet length27 ± 1.7 (24.0–28.8)-31.0 ± 5.6 (28–33)-
Conus length17.1 ± 2.5 (14.7–19.5)---
m71.1 ± 1.5 (65.1–74.2)---
DGO3.5 ± 0.5 (3.2–4.2)- -
Anterior end to excretory pore63.4 ± 3.0 (58–67)61.0 ± 8.4 (55–67)59–6858–64
Pharynx length74.6 ± 5.9 (68.5–81.2)72.0 ± 9.8 (65–79)--
Maximum body width12.9 ± 0.8 (12.0–14.0)10.9 ± 3.1 (10.1–12.5)--
Vulva body width12.2 ± 1.0 (11.5–13.5)---
Anal body width7.5 ± 2.0 (6.5–9.2)9.1 ± 0.8 (8.5–9.7)--
Head-vulva239.5 ± 8.5 (228–248)---
Head-anus275.2 ±10.1 (264–292)---
Vulva-anus39.5 ± 6.5 (29–48)---
St/L%11.4 ± 0.7 (9.5–12.3)---
Tail length15.1 ± 1.4 (14.0–17.5)16.1 ± 1.5 (15.0–17.2)15 (12–18)15.6 (15–16)
Spicule length-16.2 ± 1.7 (15.5–17.5)-14.5 (14–15)
Gubernaculum length-3.5 ± 1.7 (3.0–4.0)-2.5–3.0
DOI: https://doi.org/10.2478/jofnem-2023-0058 | Journal eISSN: 2640-396X | Journal ISSN: 0022-300X
Language: English
Submitted on: Aug 4, 2023
Published on: Dec 14, 2023
Published by: Society of Nematologists, Inc.
In partnership with: Paradigm Publishing Services
Publication frequency: 1 issue per year

© 2023 Abbas Abdolkhani, Sedighe Azimi, published by Society of Nematologists, Inc.
This work is licensed under the Creative Commons Attribution 4.0 License.