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Effect of Radopholus similis, Pratylenchus araucensis, Meloidogyne spp. and their interaction on Musa AAB ‘Dominico Hartón’ seedlings

Open Access
|Nov 2023

Figures & Tables

Figure 1:

Effect of Radopholus similis populations on Musa AAB (Plantain subgroup) ‘Dominico Hartón’ seedlings during November 2018 – April 2019 (Experiment 1) and January–July 2019 (Experiment 2). A, B: Root and shoot biomass (g dry weight), (*) indicates differences (p < 0.05) in root weight, (+) shoot weight compared to the noninfected control, according to Dunnett's test. C, D: Functional roots (%), different letters indicate difference (p = 0.05), using Tukey's test; E, F: Nematode population (nematode number/100 g fresh weight), indicated by line, and root necrosis (%) indicated by bars; and the associated Pearson correlation (r) and probability (p).
Effect of Radopholus similis populations on Musa AAB (Plantain subgroup) ‘Dominico Hartón’ seedlings during November 2018 – April 2019 (Experiment 1) and January–July 2019 (Experiment 2). A, B: Root and shoot biomass (g dry weight), (*) indicates differences (p < 0.05) in root weight, (+) shoot weight compared to the noninfected control, according to Dunnett's test. C, D: Functional roots (%), different letters indicate difference (p = 0.05), using Tukey's test; E, F: Nematode population (nematode number/100 g fresh weight), indicated by line, and root necrosis (%) indicated by bars; and the associated Pearson correlation (r) and probability (p).

Figure 2:

Effect of Pratylenchus araucensis populations on Musa AAB (Plantain subgroup) ‘Dominico Hartón’ seedlings during November 2018 – April 2019 (Experiment 1) and January–July 2019 (Experiment 2). A, B: Root and shoot biomass (g dry weight), (*) indicates differences (p < 0.05) in root weight, (+) shoot weight compared to the noninfected control, according to Dunnett's test. C, D: Functional roots (%), different letters indicate difference (p = 0.05), using Tukey's test; E, F: Nematode population (nematode number/100 g fresh weight) indicated by line, and root necrosis (%) indicated by bars; and the associated Pearson correlation (r) and probability (p).
Effect of Pratylenchus araucensis populations on Musa AAB (Plantain subgroup) ‘Dominico Hartón’ seedlings during November 2018 – April 2019 (Experiment 1) and January–July 2019 (Experiment 2). A, B: Root and shoot biomass (g dry weight), (*) indicates differences (p < 0.05) in root weight, (+) shoot weight compared to the noninfected control, according to Dunnett's test. C, D: Functional roots (%), different letters indicate difference (p = 0.05), using Tukey's test; E, F: Nematode population (nematode number/100 g fresh weight) indicated by line, and root necrosis (%) indicated by bars; and the associated Pearson correlation (r) and probability (p).

Figure 3:

Effect of Meloidogyne arenaria + M. incognita (7:1 ratio) populations on Musa AAB (Plantain subgroup) ‘Dominico Hartón’ seedlings during November 2018–April 2019 (Experiment 1) and January–July 2019 (Experiment 2). A, B: Root and shoot biomass (g dry weight), (*) indicates differences (p < 0.05) in root weight, (+) shoot weight compared to the noninfected control, according to Dunnett's test. C, D: Functional roots (%), different letters indicate significant difference (p = 0.05), using Tukey's test; E, F: Nematode population (nematode number/100 g fresh weight) indicated by line, and gall indexes (%) indicated by bars; and the associated Pearson correlation (r) and probability (p).
Effect of Meloidogyne arenaria + M. incognita (7:1 ratio) populations on Musa AAB (Plantain subgroup) ‘Dominico Hartón’ seedlings during November 2018–April 2019 (Experiment 1) and January–July 2019 (Experiment 2). A, B: Root and shoot biomass (g dry weight), (*) indicates differences (p < 0.05) in root weight, (+) shoot weight compared to the noninfected control, according to Dunnett's test. C, D: Functional roots (%), different letters indicate significant difference (p = 0.05), using Tukey's test; E, F: Nematode population (nematode number/100 g fresh weight) indicated by line, and gall indexes (%) indicated by bars; and the associated Pearson correlation (r) and probability (p).

Figure 4:

Effect of the highest population of plant-parasitic nematodes on plant height and number of leaves of Musa AAB (Plantain subgroup) ‘Dominico Hartón’ seedlings, during January–July 2019. Plants infected with 3,000 nematodes/plant, from left to right: Radopholus similis, Pratylenchus araucensis, Meloidogyne spp., and mixed populations of 3,000 R. similis + 3,000 P. araucensis + 3,000 Meloidogyne spp., and noninfected plant (control).
Effect of the highest population of plant-parasitic nematodes on plant height and number of leaves of Musa AAB (Plantain subgroup) ‘Dominico Hartón’ seedlings, during January–July 2019. Plants infected with 3,000 nematodes/plant, from left to right: Radopholus similis, Pratylenchus araucensis, Meloidogyne spp., and mixed populations of 3,000 R. similis + 3,000 P. araucensis + 3,000 Meloidogyne spp., and noninfected plant (control).

Figure 5:

Concomitant effect of Radopholus similis, Pratylenchus araucensis and Meloidogyne spp. populations on Musa AAB (Plantain subgroup) ‘Dominico Hartón’ seedlings during November 2018 – April 2019 (Experiment 1) and January–July 2019 (Experiment 2). A, B: Root and shoot biomass (g dry weight), (*) indicates differences (p < 0.05) in root weight, (+) shoot weight compared to the noninfected control, according to Dunnett's test. C, D: Functional roots (%), different letters indicate difference (p = 0.05) using Tukey's test; E, F: Nematode population (nematode number/100 g fresh weight) indicated by line, and root necrosis (%) indicated by bars; and the associated Pearson correlation (r) and probability (p).
Concomitant effect of Radopholus similis, Pratylenchus araucensis and Meloidogyne spp. populations on Musa AAB (Plantain subgroup) ‘Dominico Hartón’ seedlings during November 2018 – April 2019 (Experiment 1) and January–July 2019 (Experiment 2). A, B: Root and shoot biomass (g dry weight), (*) indicates differences (p < 0.05) in root weight, (+) shoot weight compared to the noninfected control, according to Dunnett's test. C, D: Functional roots (%), different letters indicate difference (p = 0.05) using Tukey's test; E, F: Nematode population (nematode number/100 g fresh weight) indicated by line, and root necrosis (%) indicated by bars; and the associated Pearson correlation (r) and probability (p).

Plantain growth of (height and number of leaves) and number of root bases (places where roots attach to the corm) on the sucker showing lesions by nematodes on Musa AAB ‘Dominico Hartón’ inoculated with varying initial densities of Radopholus similis, Pratylenchus araucensis, Meloidogyne spp_ and the mixture of 750 R_ similis + 750 P_ araucensis + 750 Meloidogyne spp_ and the mixture of other proportions (1,500, 2,250 and 3,000 of each species of nematodes)_

EXPNematode populationRadopholus similisPratylenchus araucensisMeloidogyne spp.Concomitant nematode infestation




Height (cm)Leaf (#)Root base (#)Height (cm)Leaf (#)Root base (#)Height (cm)Leaf (#)Root base (#)Height (cm)Leaf (#)Root base (#)




LesionTotalLesionTotalLesionTotalLesionTotal
1Controly43 ax5 a02743 a5 a02743 a5 a02743 a5 a027
75037 b6 a182536 b5 a132136 b5 a142335 b5 a1620
1,50037 b5 a172137 b5 a162438 b5 a142038 a5 a1823
2,25037 b6 a182040 b5 a172640 b5 a162337 a4 a1921
3,00038 b5 a202437 b5 a142240 b6 a152032 a5 a1820
2Control29 a6 a02629 a6 a02629 a6 a02629 a6 a026
75026 b4 b112128 b4 b51931 a4 b92725 b4 b1320
1,50025 b4 b142128 ab4 b82030 a4 b52327 b4 b1619
2,25028 ab4 b172227 b4 b61927 a4 b51825 b4 b1821
3,00025 b4 b151831 a4 b82523 b4 b61525 b4 b1821
DOI: https://doi.org/10.2478/jofnem-2023-0054 | Journal eISSN: 2640-396X | Journal ISSN: 0022-300X
Language: English
Submitted on: Mar 19, 2023
Published on: Nov 24, 2023
Published by: Society of Nematologists, Inc.
In partnership with: Paradigm Publishing Services
Publication frequency: 1 issue per year

© 2023 O. A. Guzmán-Piedrahita, C. Zamorano-Montañez, J. Leguizamon-Caycedo, B. L. Castro-Caicedo, H. D. Lopez-Nicora, published by Society of Nematologists, Inc.
This work is licensed under the Creative Commons Attribution 4.0 License.