Characterization of Virulence Phenotypes of Heterodera glycines during 2020 in Indiana

Sample ID	County	Female index (FI)^a								Mean^b	HG Type
Sample ID	County	PI 548402 #1	PI 88788 #2	PI 90763 #3	PI 437654 #4	PI 209332 #5	PI 89772 #6	PI 548316 #7	PI 567516C	Mean^b	HG Type
48	Adams	7.6	34.2	2.6	1.5	26.4	1.4	37.6	0.0	217	2.5.7
44	Benton	0.4	30.1	15.6	0.0	49.2	0.0	42.3	0.0	666	2.3.5.7
82	Carroll	1.5	73.5	0.2	0.0	44.6	0.2	28.0	14.4	249	2.5.7^c
83	Carroll	19.2	64.3	0.4	0.0	70.4	0.0	84.4	9.4	110	1.2.5.7
96	Carroll	2.4	84.8	0.5	1.3	75.7	1.5	80.0	4.4	168	2.5.7
4	DeKalb	0.5	70.5	0.0	0.0	46.6	0.3	57.0	9.6	223	2.5.7
34	DeKalb	4.7	26.5	6.0	6.8	26.0	3.3	51.5	3.1	262	2.5.7
12	Fountain	0.6	37.5	0.1	0.3	25.4	0.5	32.3	5.7	236	2.5.7
69	Fulton	0.7	62.8	0.3	0.0	58.5	0.8	69.6	0.0	371	2.5.7
33	Grant	2.1	12.5	0.5	0.4	19.1	0.4	22.4	12.7	572	2.5.7^c
50	Grant	0.0	23.7	0.0	0.0	38.0	0.0	27.7	11.8	102	2.5.7^c
51	Grant	16.8	65.2	1.0	1.1	57.4	0.1	57.9	7.5	306	1.2.5.7
92	Hancock	2.1	32.2	0.7	0.0	23.4	0.1	40.8	1.9	407	2.5.7
18	Huntington	2.1	21.0	0.6	3.0	22.3	0.8	68.0	0.3	488	2.5.7
21	Huntington	12.6	23.7	3.0	0.0	34.0	4.5	65.4	26.0	170	1.2.5.7^c
27	Huntington	0.9	24.1	1.1	1.1	33.0	0.7	12.3	8.7	68^*	2.5.7
29	Huntington	5.4	24.4	0.0	0.2	34.9	0.0	62.3	11.9	319	2.5.7^c
30	Huntington	0.8	15.7	0.1	0.0	15.8	0.1	47.3	3.0	527	2.5.7
6	Jasper	4.4	64.0	0.9	1.7	35.4	0.8	42.5	8.2	93^*	2.5.7
117	Jennings	0.6	8.5	0.0	0.0	16.0	0.0	12.1	18.8	94^*	5.7^c
78	Knox	1.8	11.4	0.5	1.2	1.2	1.5	22.3	20.5	178	2.7^c
85	Knox	0.0	6.7	0.0	0.3	5.6	0.0	16.9	0.0	142	7
77	Kosciusko	6.2	70.6	20.3	3.7	43.6	2.0	15.2	2.9	399	2.3.5.7
118	Lawrence	0.7	3.5	0.0	0.0	12.6	0.0	15.3	2.6	228	5.7
108	Noble	2.2	50.4	0.4	0.0	73.7	0.0	33.7	1.6	179	2.5.7
109	Noble	3.1	16.9	0.3	0.1	47.2	0.4	49.3	7.7	681	2.5.7
110	Noble	1.4	5.8	2.0	0.6	4.3	0.4	14.5	2.9	325	7
112	Noble	2.5	58.0	0.5	1.6	24.7	0.0	16.9	0.9	95^*	2.5.7
121	Owen	2.3	3.9	0.4	0.0	14.1	0.0	25.5	3.8	331	5.7
11	Parke	1.1	16.1	0.2	1.1	17.6	0.4	24.3	0.0	602	2.5.7
104	Porter	1.6	30.2	0.9	0.3	46.5	0.0	66.1	5.9	291	2.5.7
123	Porter	1.0	77.6	1.9	0.0	69.1	0.3	72.3	37.7	180	2.5.7^c
54	Pulaski	13.0	46.5	4.7	0.0	35.6	2.6	47.7	2.1	80^*	1.2.5.7
9	Putnam	6.7	70.5	3.8	2.1	76.2	6.6	62.3	0.0	126	2.5.7
88	Rush	2.5	15.3	0.1	0.1	20.3	0.3	24.1	3.6	872	2.5.7
61	Tippecanoe	1.9	17.6	0.9	1.3	3.8	0.2	9.0	1.2	511	2
71	Tippecanoe	0.3	80.8	0.2	0.1	45.6	0.0	63.1	3.1	243	2.5.7
56	Vigo	5.1	62.7	0.0	0.0	57.5	9.1	78.1	5.7	213	2.5.7
115	Warren	5.2	35.9	0.9	0.0	33.9	0.0	34.8	3.7	216	2.5.7
80	White	38.1	69.1	0.0	0.0	61.8	5.4	59.3	1.9	179	1.2.5.7
53	Whitley	18.4	72.5	0.0	0.0	42.6	6.1	74.4	2.3	165	1.2.5.7
94	Whitley	1.5	36.6	0.2	0.3	17.5	0.0	31.0	5.8	557	2.5.7

a FI = (average number of females on an indicator line/average number of females on the susceptible check Williams 82) × 100.

b Average number of females on the susceptible check Williams 82. SCN populations with an average number of females on Williams 82 less than 100 were indicated with ^*.

c SCN population that had an FI >10% on PI 567516C.

A map of counties in Indiana where *Heterodera glycines* virulence phenotypes were tested using soil samples collected in 2020. The *H. glycines* virulence type(s) found in each county tested are labeled on the map.

Table 2.

Univariate descriptive statistics of female indices (FI) of soybean cyst nematode populations that produced an FI ≥ 10% on soybean indicator lines

Soybean Indicator Line	FI value (%)^b
Soybean Indicator Line	FI ≥ 10%^a	Minimum Observed	Maximum Observed	Average
PI 548402 (#1)	14.3	12.6	38.1	19.7
PI 88788 (#2)	88.1	11.4	84.8	43.9
PI 90763 (#3)	4.8	20.3	25.5	22.9
PI 209332 (#5)	90.5	12.6	76.2	39.8
PI 548316 (#7)	97.6	12.1	84.4	43.8
PI 567516C	19.0	11.8	37.7	19.2

a Percentage of SCN populations with an FI ≥ 10%.

b Only FI ≥ 10% were included.

Table 3.

Frequencies of HG types in soil samples collected in 2020 in Indiana

Type	No. of Populations^a	Frequency (%)
2.5.7	27	64.3
1.2.5.7	6	14.3
5.7	3	7.1
2.3.5.7	2	4.8
7	2	4.8
2.7	1	2.4
2	1	2.4

a Number of soybean cyst nematode populations (out of 42 tested in this study).

Table 4.

Correlation coefficient among soybean indicator lines with resistance to soybean cyst nematode (SCN) based on female indices (FI)^a from 42 SCN field populations collected in 2020 in Indiana

Indicator Line	PI 548402	PI 88788	PI 90763	PI 437654	PI 209332	PI 89772	PI 548316
PI 88788	0.360^*
PI 90763	−0.022 ns	0.057 ns
PI 437654	−0.037 ns	0.028 ns	0.303 ns
PI 209332	0.345^*	0.799^**	0.120 ns	−0.100 ns
PI 89772	0.501^**	0.342^*	0.051 ns	0.169 ns	0.311^*
PI 548316	0.396^**	0.591^**	−0.091 ns	−0.048 ns	0.675^**	0.431^**
PI 567516C	−0.050 ns	0.045 ns	−0.140 ns	−0.202 ns	0.111 ns	−0.051 ns	0.114 ns

a FI = (average number of females on an indicator line/average number of females on the susceptible check Williams 82) × 100.

* P < 0.05;

** P < 0.01

ns: not significant (P > 0.05)

Supplementary Table 1:

Population densities of soybean cyst nematode in soil samples collected in Indiana in 2020

County	Number of soil samples^a	Eggs and Juveniles
County	Number of soil samples^a	Minimum No.^b	Maximum No.^c	Average No.^d
Adams	2	2500	5505	4003
Allen	3	1370	2500	1940
Benton	7	300	4300	2098
Carroll	7	665	13308	5254
Clinton	1	600	600	600
DeKalb	6	240	15600	4782
Fayette	4	400	10950	5316
Fountain	2	4950	10650	7800
Fulton	2	3320	6050	4685
Grant	4	400	6650	3358
Hancock	2	783	1050	916
Henry	2	417	483	450
Huntington	16	240	7265	1805
Jasper	4	220	17032	5669
Jennings	2	290	530	410
Knox	5	420	2100	1129
Kosciusko	1	10043	10043	10043
LaPorte	3	356	2280	1026
Lawrence	2	400	665	532
Montgomery	2	940	2610	1775
Noble	7	1020	19840	8313
Owen	2	290	300	295
Parke	1	2075	2075	2075
Porter	4	312	5940	2278
Pulaski	2	9045	10330	9688
Putnam	3	2425	13285	8070
Rush	2	853	2020	1437
Tippecanoe	14	165	6647	1418
Vigo	1	2120	2120	2120
Warren	1	765	765	765
White	4	550	6040	2888
Whitley	6	400	7040	2163

a Total number of soil samples collected in a county.

b Minimum number of SCN eggs and juveniles per 100 cm³ of soil.

c Maximum number of SCN eggs and juveniles per 100 cm³ of soil.

d Average number of SCN eggs and juveniles per 100 cm³ of soil.

Articles in this issue

DOI: https://doi.org/10.2478/jofnem-2023-0039 | Journal eISSN: 2640-396X | Journal ISSN: 0022-300X

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Language: English

Submitted on: Apr 26, 2023

Published on: Oct 4, 2023

Published by: Society of Nematologists, Inc.

In partnership with: Paradigm Publishing Services

Publication frequency: 1 issue per year

Keywords:

soybean cyst nematode,

virulence phenotype,

HG type,

soybean,

nematode management

Related subjects:

Life sciences,

Life sciences, other

© 2023 Ricky Critchfield, Jaden King, John Bonkowski, Darcy Telenko, Tom Creswell, Lei Zhang, published by Society of Nematologists, Inc.
This work is licensed under the Creative Commons Attribution 4.0 License.

Volume 55 (2023): Issue 1 (February 2023)

Characterization of Virulence Phenotypes of Heterodera glycines during 2020 in Indiana

Figures & Tables

Table 1.

Figure 1:

Table 2.

Table 3.

Table 4.

Supplementary Table 1:

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