Figure 1:
Figure 2:
Figure 3:
Figure 4:
Comparison of important morphological character and morphometrics of the Ethiopian Quinisulcius capitatus, found from chickpea in Ethiopia, with original description from USA (Allen, 1955), and two other Q_ capitatus from Ethiopia and Canada_ All measurements are in μm and in the form: mean ± s_d_ (range)_
| Character | Q. capitatus from chickpea in Ethiopia (present study) | Q. capitatus from pear in USA (Allen, 1955) | Q. capitatus from coffee in Ethiopia (Mekete et al., 2008) | “Q. capitatus”* from grass in Canada (Munawar et al., 2021) |
|---|---|---|---|---|
| n | 10 | 13 | 10 | 20 |
| L | 699 ± 11.6 (667 – 707) | 630 – 850 | 630 – 790 | 810.3 ± 44.6 (744.0 – 911.0) |
| a | 31.2 ± 1.3 (29.7 – 34.4) | 30 – 38 | 30.9 – 38.6 | 41.4 ± 1.8 (38.6 – 43.7) |
| b | 5.4 ± 0.2 (5.1 – 5.7) | 5.0 – 5.8 | – | 5.5 ± 0.3 (5.0 – 6.3) |
| c | 14.7 ± 0.7 (14.1 – 16.3) | 12 – 17 | 15.3 – 17.6 | 22.4 ± 1.1 (19.9 – 23.8) |
| c’ | 1.9 ± 0.2 (1.5 – 2.1) | – | – | 2.6 ± 0.2 (2.2 – 3.2) |
| V | 56.6 ± 2.2 (52.7 – 59.0) | 51 – 58 | 54.7 – 63.6 | 57.4 ± 1.5 (53.4 – 59.8) |
| Stylet length | 18.8 ± 0.6 (17.8 – 19.7) | 16 – 18 | 15 – 18 | 18.3 ± 1.0 (15.5 – 20.4) |
| Lip height | 4.0 ± 0.5 (3.2 – 4.5) | – | – | 4.0 ± 0.2 (3.7 – 4.4) |
| Lip width | 7.6 ± 0.5 (7.0 – 8.3) | – | – | 7.6 ± 0.4 (6.9 – 8.3) |
| Median bulb length | 16.1 ± 0.3 (15.7 – 16.6) | – | – | 14.0 ± 1.6 (11.3 – 16.9) |
| Median bulb width | 13.1 ± 0.6 (12.2 – 13.9) | – | – | 10.4 ± 1.4 8.4 – 14.2 |
| Pharyngeal length | 129 ± 5.1 (123 – 138) | – | – | 147.8 ± 5.8 (140.2 – 159.0) |
| SE pore from anterior end | 121 ± 3.3 (113 – 123) | – | 121 | 128.6 ± 5.3 (121.0 – 139.0) |
| Midbody diameter | 22.4 ± 1.0 (20.5 – 23.5) | 21 – 27 | – | – |
| Tail length | 47.8 ± 3.0 (41.0 – 50.0) | – | – | 35.8 ± 2.4 (31.3 – 40.4) |
| Anal body diameter | 25.3 ± 3.2 (23.0 – 34.2) | – | – | – |
| Phasmid position | Middle of tail | Middle of tail | – | Middle of tail |
Pratylenchus delattrei and Quinisulcius capitatus recovered from chickpea roots and soil, districts, sampling locality, laboratory codes, altitude, GPS coordinators and GenBank accession numbers_
| Districts | Sampling locality | Codes | Species | Altitude (m) | Longitude(°) | Latitude(°) | GenBank accession number | ||
|---|---|---|---|---|---|---|---|---|---|
| 28S–rDNA | ITS–rDNA | COI gene | |||||||
| Minjar | Kitecha | Mki-5 | P. delattrei | 1600-1800 | 8°52′58.16″N | 39°29′46.01″E | OP646170 | - | - |
| Mki-8 | OP646169 | - | - | ||||||
| Mki-12 | - | - | OP730534 | ||||||
| Adea’a | Gollodhertu | AG-1 | P. delattrei | 1800-1900 | 8°38′55.54″N | 38°55′0.94″E | - | OP646171 | - |
| AG-2 | OP646168 | OP646172 | - | ||||||
| AG-3 | OP646167 | - | OP730535 | ||||||
| Mesekan | Jolle-2 | JO2-3 | Q. capitatus | 1900-1950 | 8°11′49.79″N | 38°27′51.24″E | OP626319 | OP646173 | OP627909 |
| JO2-5 | OP626320 | OP646174 | OP627910 | ||||||
| JO2-7 | OP626321 | OP646175 | OP627911 | ||||||
Comparison of morphometrics of the Ethiopian Pratylenchus delattrei from chickpea in Ethiopia with the original description from Madagascar (Luc, 1958), and two other P_ delattrei populations from Cape Verde and Iran_ All measurements are in μm and in the form: mean ± s_d_ (range)_
| Character | P. delattrei from chickpea in Ethiopia (present study) | P. delattrei from cotton in Madagascar (Luc, 1958) | P. delattrei from tomato in Cape Verde (Flis et al., 2018) | P. delattrei from vegetables in Iran (Majd Taheri et al., 2013) | |
|---|---|---|---|---|---|
| Hormozgan 1 | Hormozgan 2 | ||||
| n | 10 | 13 | 20 | 7 | 12 |
| L | 475 ± 47.1 (410 – 560) | 390 – 470 | 532 ± 33 (498 – 586) | 543 ± 55 (467 – 616) | 508 ± 49.2 (434 – 576) |
| a | 25.2 ± 2.2 (22.8 – 29) | 20.4 – 25.8 | 26.6 ± 2.2 (22.1 – 31.3) | 23.8 ± 2.1 (21.2 – 26.9) | 22.6 ± 1.0 (21.1 – 25) |
| b | 5.6 ± 0.6 (4.8 – 6.6) | 3.7 – 4.8 | 6.6 ± 0.5 (6.1 – 7.7) | 6.1 ± 0.7 (5.0 – 7.2) | 5.9 ± 0.6 (5.2 – 6.9) |
| b’ | 4.7 ± 0.5 (4.1 – 5.6) | – | 4.5 ± 0.4 (4.0 – 5.3) | 4.3 ± 0.2 (4.0 – 4.6) | 4.1 ± 0.4 (3.6 – 4.9) |
| c | 14.6 ± 1.9 (13.7 – 20.3) | 18 – 22.3 | 21.9 ± 2.1 (18.5 – 25.1) | 20 ± 2 (18.1 – 23.1) | 19.7 ± 2.6 (16.7 – 24.1) |
| c’ | 2.3 ± 0.2 (2.0 – 2.8) | – | 2.2 ± 0.2 (1.9 – 2.8) | 2.2 ± 0.3 (1.9 – 2.6) | 1.9 ± 0.2 (1.6 – 2.2) |
| V | 72.2 ± 7.6 (62.4 – 86.9) | 73 – 81 | 76 ± 1 (75 – 78) | 75.1 ± 1.9 (71.4 – 77.1) | 75.9 ± 1.3 (74 – 78.7) |
| Stylet length | 16.7 ± 0.7 (15.8 – 17.8) | 16.5 – 18.0 | 16.4 ± 0.4 (15.4 – 16.9) | 16.3 ± 0.8 (15 – 17) | 16.0 ± 0.6 (15 – 17) |
| Dorsal gland opening from stylet base | 3.0 ± 0.5 (2.3 – 3.5) | – | 2.9 ± 0.3 (2.4 – 3.1) | – | – |
| O | 17.7 ± 2.9 (13.6 – 22.2) | – | 17.4 ± 1.7 (14.3 – 21.3) | – | – |
| Pharynx length | 85 ± 1.0 (82.5 – 85.9) | – | 80.1 ± 3.2 (73.1 – 84.2) | 89 ± 10.4 (76 – 105) | 87 ± 4 (82 – 94) |
| Pharyngeal overlap | – | – | 39.0 ± 7.2 (29.8 – 49.0) | – | |
| Anterior end to end of pharyngeal gland lobe | 101 ± 1.4 (98 – 102) | – | – | 125 ± 7.3 (116 – 135) | 123 ± 8.3 (113 – 138) |
| Maximal body diameter | 22.7 ± 0.5 (21.6 – 23.1) | – | 20.2 ± 2.2 (16.8 – 23.7) | 23 ± 3.3 (20 – 29) | 22.5 ± 2.1 (19 – 26) |
| Anal body diameter | 13.0 ± 1.2 (10.9 – 14.1) | – | 11.2 ± 1.2 (8.9 – 13.2) | 12.4 ± 1.5 (11 – 14) | 13 ± 0.9 (12 – 14) |
| Tail length | 29.0 ± 0.9 (27.5 – 30.1) | – | 24.8 ± 2.3 (21.0 – 27.1) | 26.6 ± 2.3 (23 – 29) | 24.8 ± 2.3 (23 – 29) |
| Tail annuli | 20 ± 2 (18 – 23) | – | 19 ± 2 (16 – 24) | 20 ± 2.1 (18 – 23) | 19 ± 1.5 (17 – 21) |
| Phasmid to terminus | 14.1 ± 0.4 (13.5 – 14.5) | – | 10.6 ± 2.7 (6.0 – 14.9) | – | – |