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Virulence of Two Isolates of Meloidogyne enterolobii (Guava Root-Knot Nematode) from North Carolina on Cotton Lines Resistant to Southern Root-Knot Nematode (M. incognita) and Reniform Nematode (Rotylenchulus reniformis)

Journal of Nematology
Volume 55 (2023): Issue 1 (February 2023)
By:
Open Access
|Jun 2023

Figures & Tables

Figure 1:

Virulence of Meloidogyne enterolobii isolates 18-5126 and NC.1 on cotton germplasm lines resistant to M. incognita (RKN) (M240 RNR), resistant to RN (M713 Ren1), or resistant to both RKN and RN (M Rk-Rn-1) in comparison to a greenhouse-maintained RKN race 3 population. Also included were nematode-susceptible obsolete cultivars Deltapine61 (DPL61) and SureGrow747 (SG747). Eggs were used as inoculum and virulence was measured as (A) RF, (B) log10 eggs g−1 root, and (C) gall score index (1–10 scale). Tukey-Kramer multiple comparison was used to determine significant differences between means at α = 0.05 as denoted by different letters (n = 6).
Virulence of Meloidogyne enterolobii isolates 18-5126 and NC.1 on cotton germplasm lines resistant to M. incognita (RKN) (M240 RNR), resistant to RN (M713 Ren1), or resistant to both RKN and RN (M Rk-Rn-1) in comparison to a greenhouse-maintained RKN race 3 population. Also included were nematode-susceptible obsolete cultivars Deltapine61 (DPL61) and SureGrow747 (SG747). Eggs were used as inoculum and virulence was measured as (A) RF, (B) log10 eggs g−1 root, and (C) gall score index (1–10 scale). Tukey-Kramer multiple comparison was used to determine significant differences between means at α = 0.05 as denoted by different letters (n = 6).

Figure 2:

Virulence of Meloidogyne enterolobii isolates 18-5126 and NC.1 on cotton germplasm lines resistant to M. incognita (RKN) (M240 RNR), resistant to RN (M713 Ren1), or resistant to both RKN and RN (M Rk-Rn-1) using second-stage juveniles as inoculum. Also included were nematode-susceptible obsolete cultivars Deltapine61 (DPL61) and SureGrow747 (SG747). Virulence was measured as (A) RF ± SE, (B) log10 eggs g−1 root ± SE, and (C) gall score index ± SE (1–10 scale) of six replicates.
Virulence of Meloidogyne enterolobii isolates 18-5126 and NC.1 on cotton germplasm lines resistant to M. incognita (RKN) (M240 RNR), resistant to RN (M713 Ren1), or resistant to both RKN and RN (M Rk-Rn-1) using second-stage juveniles as inoculum. Also included were nematode-susceptible obsolete cultivars Deltapine61 (DPL61) and SureGrow747 (SG747). Virulence was measured as (A) RF ± SE, (B) log10 eggs g−1 root ± SE, and (C) gall score index ± SE (1–10 scale) of six replicates.

Figure 3:

Time-point dependent reproduction of the Meloidogyne enterolobii isolate NC.1 on cotton germplasm lines M240 RNR and MRk-Rn-1 in comparison to the susceptible cultivar SureGrow747 (SG747). Total eggs were extracted at 35-, 42-, 49-, and 56-days post inoculation (DPI). Isolate NC.1 reproduction is presented as (A) RF (total eggs extracted/initial inoculum) and (B) log10 eggs g−1 root. Significant differences between means at α = 0.05 noted by different letters (n = 6).
Time-point dependent reproduction of the Meloidogyne enterolobii isolate NC.1 on cotton germplasm lines M240 RNR and MRk-Rn-1 in comparison to the susceptible cultivar SureGrow747 (SG747). Total eggs were extracted at 35-, 42-, 49-, and 56-days post inoculation (DPI). Isolate NC.1 reproduction is presented as (A) RF (total eggs extracted/initial inoculum) and (B) log10 eggs g−1 root. Significant differences between means at α = 0.05 noted by different letters (n = 6).

Figure 4:

Time-course of Meloidogyne enterolobii isolate NC.1 infection of SureGrow747 and MRk-Rn-1. Infected roots were collected at 4-, 8-, 12-, 16-, 20-, and 24-days after inoculation (DAI) and stained with acid fuchsin.
Time-course of Meloidogyne enterolobii isolate NC.1 infection of SureGrow747 and MRk-Rn-1. Infected roots were collected at 4-, 8-, 12-, 16-, 20-, and 24-days after inoculation (DAI) and stained with acid fuchsin.

Effect of Meloidogyne enterolobii infection on cotton growth under growth chamber conditions_ Presented are mean ± SE after 6 weeks of growth under uninoculated (control) or inoculated conditions (n = 5)_

ControlInoculated
Stem Height (mm)DPL61124.1 ± 3.279.3 ± 3.7**
M240 RNR147.8 ± 4.6133.8 ± 22.8
SG747143.5 ± 2.889.6 ± 2.1**
M713 Ren1144.0 ± 17.0131.0 ± 12.8
MRk-Rn-1157.6 ± 10.5111.6 ± 5.4**
Shoot Fresh Weight (g)DPL612.49 ± 0.131.94 ± 0.23
M240 RNR2.75 ± 0.232.01 ± 0.27
SG7473.23 ± 0.451.48 ± 0.19**
M713 Ren12.58 ± 0.223.39 ± 0.42
MRk-Rn-12.99 ± 0.741.83 ± 0.11
Leaf NumberDPL613.7 ± 0.13.8 ± 0.4
M240 RNR3.8 ± 0.13.5 ± 0.5
SG7473.5 ± 0.22.4 ± 0.3**
M713 Ren13.9 ± 0.33.8 ± 0.3
MRk-Rn-14.1 ± 0.33.1 ± 0.2**
Stem Height/Node NumberDPL6128.9 ± 0.521.1 ± 1.8**
M240 RNR33.2 ± 1.933.2 ± 1.5
SG74740.0 ± 2.326.5 ± 0.9**
M713 Ren131.7 ± 4.727.6 ± 2.2
MRk-Rn-140.5 ± 2.329.5 ± 1.1**
DOI: https://doi.org/10.2478/jofnem-2023-0021 | Journal eISSN: 2640-396X | Journal ISSN: 0022-300X
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Language: English
Submitted on: Aug 26, 2022
Published on: Jun 23, 2023
Published by: Society of Nematologists, Inc.
In partnership with: Paradigm Publishing Services
Publication frequency: 1 issue per year
Keywords:
Cotton,
resistance,
root-knot nematode,
Meloidogyne
Related subjects:
Life sciences,
Life sciences, other

© 2023 Amanda G. Gaudin, Martin J. Wubben, Jack C. McCarty, Johnie N. Jenkins, published by Society of Nematologists, Inc.
This work is licensed under the Creative Commons Attribution 4.0 License.

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