Figure 1:
Virulence of Meloidogyne enterolobii isolates 18-5126 and NC.1 on cotton germplasm lines resistant to M. incognita (RKN) (M240 RNR), resistant to RN (M713 Ren1), or resistant to both RKN and RN (M Rk-Rn-1) in comparison to a greenhouse-maintained RKN race 3 population. Also included were nematode-susceptible obsolete cultivars Deltapine61 (DPL61) and SureGrow747 (SG747). Eggs were used as inoculum and virulence was measured as (A) RF, (B) log10 eggs g−1 root, and (C) gall score index (1–10 scale). Tukey-Kramer multiple comparison was used to determine significant differences between means at α = 0.05 as denoted by different letters (n = 6).
Figure 2:
Virulence of Meloidogyne enterolobii isolates 18-5126 and NC.1 on cotton germplasm lines resistant to M. incognita (RKN) (M240 RNR), resistant to RN (M713 Ren1), or resistant to both RKN and RN (M Rk-Rn-1) using second-stage juveniles as inoculum. Also included were nematode-susceptible obsolete cultivars Deltapine61 (DPL61) and SureGrow747 (SG747). Virulence was measured as (A) RF ± SE, (B) log10 eggs g−1 root ± SE, and (C) gall score index ± SE (1–10 scale) of six replicates.
Table 1.
Effect of Meloidogyne enterolobii infection on cotton growth under growth chamber conditions. Presented are mean ± SE after 6 weeks of growth under uninoculated (control) or inoculated conditions (n = 5).
| Control | Inoculated | ||
|---|---|---|---|
| Stem Height (mm) | DPL61 | 124.1 ± 3.2 | 79.3 ± 3.7** |
| M240 RNR | 147.8 ± 4.6 | 133.8 ± 22.8 | |
| SG747 | 143.5 ± 2.8 | 89.6 ± 2.1** | |
| M713 Ren1 | 144.0 ± 17.0 | 131.0 ± 12.8 | |
| MRk-Rn-1 | 157.6 ± 10.5 | 111.6 ± 5.4** | |
| Shoot Fresh Weight (g) | DPL61 | 2.49 ± 0.13 | 1.94 ± 0.23 |
| M240 RNR | 2.75 ± 0.23 | 2.01 ± 0.27 | |
| SG747 | 3.23 ± 0.45 | 1.48 ± 0.19** | |
| M713 Ren1 | 2.58 ± 0.22 | 3.39 ± 0.42 | |
| MRk-Rn-1 | 2.99 ± 0.74 | 1.83 ± 0.11 | |
| Leaf Number | DPL61 | 3.7 ± 0.1 | 3.8 ± 0.4 |
| M240 RNR | 3.8 ± 0.1 | 3.5 ± 0.5 | |
| SG747 | 3.5 ± 0.2 | 2.4 ± 0.3** | |
| M713 Ren1 | 3.9 ± 0.3 | 3.8 ± 0.3 | |
| MRk-Rn-1 | 4.1 ± 0.3 | 3.1 ± 0.2** | |
| Stem Height/Node Number | DPL61 | 28.9 ± 0.5 | 21.1 ± 1.8** |
| M240 RNR | 33.2 ± 1.9 | 33.2 ± 1.5 | |
| SG747 | 40.0 ± 2.3 | 26.5 ± 0.9** | |
| M713 Ren1 | 31.7 ± 4.7 | 27.6 ± 2.2 | |
| MRk-Rn-1 | 40.5 ± 2.3 | 29.5 ± 1.1** |
Figure 3:
Time-point dependent reproduction of the Meloidogyne enterolobii isolate NC.1 on cotton germplasm lines M240 RNR and MRk-Rn-1 in comparison to the susceptible cultivar SureGrow747 (SG747). Total eggs were extracted at 35-, 42-, 49-, and 56-days post inoculation (DPI). Isolate NC.1 reproduction is presented as (A) RF (total eggs extracted/initial inoculum) and (B) log10 eggs g−1 root. Significant differences between means at α = 0.05 noted by different letters (n = 6).
Figure 4:
Time-course of Meloidogyne enterolobii isolate NC.1 infection of SureGrow747 and MRk-Rn-1. Infected roots were collected at 4-, 8-, 12-, 16-, 20-, and 24-days after inoculation (DAI) and stained with acid fuchsin.