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Morphological and Molecular Characterization of Diplogasteroides sp., a Cryptic Population of the Haslacheri Group (Diplogastridae), and Parasitorhabditis terebranus (Rhabditidae) from Korea

Journal of Nematology
Volume 55 (2023): Issue 1 (February 2023)
By:
Open Access
|May 2023

Figures & Tables

Figure 1:

Photomicrographs of Diplogasteroides sp. (A-C, F, and G) and Parasitorhabditis terebranus (D, E, and H-J). Diplogasteroides sp. A, B: Variation in female anterior body region; C: Female tail; F and G: Variation in male tail and copulatory apparatus shape. Parasitorhabditis terebranus D, E, and H: Variation in female anterior body region; I: Male tail with copulatory apparatus; J: Female tail; (Scale bars: A-J = 10 μm).
Photomicrographs of Diplogasteroides sp. (A-C, F, and G) and Parasitorhabditis terebranus (D, E, and H-J). Diplogasteroides sp. A, B: Variation in female anterior body region; C: Female tail; F and G: Variation in male tail and copulatory apparatus shape. Parasitorhabditis terebranus D, E, and H: Variation in female anterior body region; I: Male tail with copulatory apparatus; J: Female tail; (Scale bars: A-J = 10 μm).

Figure 2:

Illustrations of Diplogasteroides sp. (A-H): A: Female whole body; B: Male whole body; C: Male tail region including copulatory apparatus and the arrangement of genital papillae; D-F: Variation in shape of gubernaculum; G: Female tail region; H: Female anterior region, including the shape of pharynx. Abbreviations: Genital papillae arrangement from ventral side (v + number); lateral/dorsal sides (v3d; ad; pd); phasmid (ph); and deirid (d).
Illustrations of Diplogasteroides sp. (A-H): A: Female whole body; B: Male whole body; C: Male tail region including copulatory apparatus and the arrangement of genital papillae; D-F: Variation in shape of gubernaculum; G: Female tail region; H: Female anterior region, including the shape of pharynx. Abbreviations: Genital papillae arrangement from ventral side (v + number); lateral/dorsal sides (v3d; ad; pd); phasmid (ph); and deirid (d).

Figure 3:

Illustrations of Parasitorhabditis terebranus (A-G): A: Male whole body; B: Female whole body; C: Female anterior region; D and F: Female tail region including variation in vulval lip shape; E: Male tail in right lateral view, with arrangement of bursal rays; G: Male tail in ventral view, with arrangement of bursal rays.
Illustrations of Parasitorhabditis terebranus (A-G): A: Male whole body; B: Female whole body; C: Female anterior region; D and F: Female tail region including variation in vulval lip shape; E: Male tail in right lateral view, with arrangement of bursal rays; G: Male tail in ventral view, with arrangement of bursal rays.

Figure 4:

Bayesian tree inferred under the GTR + I + G model from I8S-rRNA sequences of Diplogasteroides spp., Parasitorhabditis spp., and other closely related species from other genera. Posterior probability values exceeding 50% are given on appropriate clades. The studied population is indicated in bold. Outgroup taxon: Rhabditoides inermiformis and Leptolaimus donsi.
Bayesian tree inferred under the GTR + I + G model from I8S-rRNA sequences of Diplogasteroides spp., Parasitorhabditis spp., and other closely related species from other genera. Posterior probability values exceeding 50% are given on appropriate clades. The studied population is indicated in bold. Outgroup taxon: Rhabditoides inermiformis and Leptolaimus donsi.

Figure 5:

Bayesian tree inferred under the GTR + I + G model from D2-D3 expansion segment of 28S-rRNA partial sequences of Diplogasteroides spp., Parasitorhabditis spp., and other closely related species from other genera. Posterior probability values exceeding 50% are given on appropriate clades. The studied population is indicated in bold. Outgroup taxon: Rhabditoides regina.
Bayesian tree inferred under the GTR + I + G model from D2-D3 expansion segment of 28S-rRNA partial sequences of Diplogasteroides spp., Parasitorhabditis spp., and other closely related species from other genera. Posterior probability values exceeding 50% are given on appropriate clades. The studied population is indicated in bold. Outgroup taxon: Rhabditoides regina.

Figure 6:

Bayesian tree inferred under the GTR + I + G model from COI partial sequences of Diplogasteroides spp., Parasitorhabditis spp., and members of closely related genera. Posterior probability values exceeding 50% are given on appropriate clades. The studied population is indicated in bold. Outgroup taxon: Ascaridia columbae.
Bayesian tree inferred under the GTR + I + G model from COI partial sequences of Diplogasteroides spp., Parasitorhabditis spp., and members of closely related genera. Posterior probability values exceeding 50% are given on appropriate clades. The studied population is indicated in bold. Outgroup taxon: Ascaridia columbae.

Comparison of morphometries of Diplogasteroides sp_ and Parasitorhabditis terebranus from Korea with topotype populations_a

CharacterDiplogasteroides sp.D. haslacheriParasitorhabditis terebranus
Korea (Current study)Rühm (1956), and Kanzaki et al. (2002)Korea (Current study)USA (Massey, 1974)
♀♀♂♂♀♀b♂♂♀♀♂♂♀♀♂♂
N2020??2020??
L1061,3±106.9(855.3-1258.3)909.O±82.8(777.6-1068.1)825-1005750-825109δ.4±78.6(922.4-1244.4)1015.8±96.9(841.0-1195.6)770-810750
A29.4±2.1(25.1-32.3)29.2±0.8(27.7-30.5)26.126.2-26.826.4±2.9(20.5-30.0)26.0±2.2(21.1-29.4)19.7-20.119.5
B6.2±0.5(5.4-7.5)5.8±0.4(5.2-6.3)5.6-6.15.1-5.25.5±0.4(4.6-6.1)5.2±0.4(4.6-6.1)4.2-4.34.1
C11.7±0.8(9.8-13.1)I4.9±0.9(13.2-16.2)9.4-9.612.4-15.327.1±2.2(22.8-30.2)27.4±2.3(22.1-30.5)26.2-27.327.3
c’4.2±0.3(3.5-4.7)2.5±0.2(2.2-2.9)5.02.41.9±0.2(1.6-2.1)1.7±0.1(1.4-1.9)--
V51.6±1.1(50.3-53.2)-50.3-52.3-93.3±0.5(91.9-93.9)-93-
Lip height----3.3±0.1(3.0-3.6)3.1 ±0.2(2.8-3.5)--
Lip diameter11.9±0.5(10.6-12.6)11.9±0.6(11.0-12.6)--12.6±0.7(11.6-14.0)12.1 ±0.7(11.0-13.5)--
Stoma11.1±0.8(9.8-12.3)11.0±0.7(9.0-11.8)--20.9±1.4(18.5-23.0)20.3±1.1(18.0-22.0)21-
Anterior to median bulb valve98.2±6.3(87.6-112.7)90.4±5.3(82.4-102.2)--107.2±7.4(95.8-120.2)101,4±5.1(93.3-112.9)--
Excretory pore1340±8.3(123.0-155.4)126.8±5.7(114.8-138.0)--1540±8.7(135.0-162.7)153.6±8.6(136.0-164.0)--
Pharynx length171.4±11.9(154.1-194.2)156.3±8.6(145.0-170.0)--201.0±9.3 (191.3-223.0)194.3±8.1(181.3-208.5)--
Maximum body diameter36.2±3.5(29.0-42.1)31.7±3.0(27.4-37.7)--41.9±4.7(36.0-51.5)39.1±2.7(33.3-43.0)--
Vulval body diameter38.1±3.6(33.6-43.6)---35.2±2.9(30.8-42.6)---
Vulva to anus length----32.5±2.6(28.0-38.0)---
Vulva to tail tip----73.1±3.5(64.0-77.9)---
Anal / cloacal body diameter21.5±1.4(19.2-24.8)24.2±1.6(22.0-27.0)--21.4±1.5(18.9-24.5)21.8±1.6(19.8-24.9)--
Tail length90.5±9.5(73.0-104.6)60.8±4.6(50.0-69.5)--40.6±3.0(36.0-45.8)37.0±2.5(31.5-41.0)--
Spicules-35.5±2.1(30.0-38.0)-27-30-36.6±1.7(33.0-38.6)-34
Gubernaculum-17.9±1.5(15.7-21.0)-15-17-16.5±0.6(15.2-18.0)-17
Tail spike length-19.1 ±2.3(16.0-23.4)------
DOI: https://doi.org/10.2478/jofnem-2023-0017 | Journal eISSN: 2640-396X | Journal ISSN: 0022-300X
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Language: English
Submitted on: Jan 29, 2023
Published on: May 23, 2023
Published by: Society of Nematologists, Inc.
In partnership with: Paradigm Publishing Services
Publication frequency: 1 issue per year
Keywords:
morphology,
taxonomy
Related subjects:
Life sciences,
Life sciences, other

© 2023 Abraham Okki Mwamula, Sang Myeong Lee, Young Hak Jung, Ho-wook Lee, Yi Seul Kim, Young Ho Kim, Dong Woon Lee, published by Society of Nematologists, Inc.
This work is licensed under the Creative Commons Attribution 4.0 License.

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