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Morphological and Molecular Characterization of Filenchus multistriatus n. sp. (Tylenchomorpha: Tylenchidae) and Data on a Known Species of the Genus from Bushehr Province, Southern Iran

Journal of Nematology
Volume 55 (2023): Issue 1 (February 2023)
By:
Open Access
|May 2023

Figures & Tables

Figure 1:

Line drawings of Filenchus multistriatus n. sp. (Female). (A) Entire body. (B) Tail. (C) Pharynx. (D) Lateral view of lateral field. (E) Lateral field.
Line drawings of Filenchus multistriatus n. sp. (Female). (A) Entire body. (B) Tail. (C) Pharynx. (D) Lateral view of lateral field. (E) Lateral field.

Figure 2:

Light microphotographs of Filenchus multistriatus n. sp. (Female). (A&B) Anterior body region (arrows pointing to the excretory pore). (C) Entire body. (D) Reproductive system. (E) Tail. (F&G) Lateral field in two paratypes in lateral view.
Light microphotographs of Filenchus multistriatus n. sp. (Female). (A&B) Anterior body region (arrows pointing to the excretory pore). (C) Entire body. (D) Reproductive system. (E) Tail. (F&G) Lateral field in two paratypes in lateral view.

Figure 3:

Scanning electron microphotographs of Filenchus multistriatus n. sp. (Female). (A-C) Lip region in ventral and subfrontal views, respectively (arrows pointing to the amphidial openings). (D) Anterior body region. (E&F) Lip region in frontal view (arrows pointing to the amphidial openings). (G, I, J) Lateral field. (H) Tail (arrow pointing to the anus). (K) Vulva.
Scanning electron microphotographs of Filenchus multistriatus n. sp. (Female). (A-C) Lip region in ventral and subfrontal views, respectively (arrows pointing to the amphidial openings). (D) Anterior body region. (E&F) Lip region in frontal view (arrows pointing to the amphidial openings). (G, I, J) Lateral field. (H) Tail (arrow pointing to the anus). (K) Vulva.

Figure 4:

Light microphotographs of Iranian population of F. sandneri Wasilewska, 1965 from Bushehr province (Female). (A) Anterior body region (arrows pointing to the excretory pore). (B) Reproductive system. (C) Lip region and stylet. (D) Lateral field. (E) Tail. (F) Entire body.
Light microphotographs of Iranian population of F. sandneri Wasilewska, 1965 from Bushehr province (Female). (A) Anterior body region (arrows pointing to the excretory pore). (B) Reproductive system. (C) Lip region and stylet. (D) Lateral field. (E) Tail. (F) Entire body.

Figure 5:

Scanning electron microphotographs of Iranian population of F. sandneri Wasilewska, 1965 from Bushehr province (Female). (A-C) Lip region in ventral, subfrontal and frontal views, respectively (arrows pointing to the amphidial openings). (D) Excretory pore. (E) Lateral field. (F) Tail (upper and lower arrows pointing to the vulva and anus, respectively). (G,H) Lateral fields. (I) Vulva. (J) Anus.
Scanning electron microphotographs of Iranian population of F. sandneri Wasilewska, 1965 from Bushehr province (Female). (A-C) Lip region in ventral, subfrontal and frontal views, respectively (arrows pointing to the amphidial openings). (D) Excretory pore. (E) Lateral field. (F) Tail (upper and lower arrows pointing to the vulva and anus, respectively). (G,H) Lateral fields. (I) Vulva. (J) Anus.

Figure 6:

Bayesian 50% majority rule consensus tree inferred from SSU rDNA of Filenchus multistriatus n. sp. under the GTR + G + I model (-ln L = 20913.9590; freq A = 0.2301; freqC = 0.2419; freqG = 0.3006; freq T = 0.2273; R(a) = 0.8974; R(b) = 2.1547; R(c) = 0.9066; R(d) = 1.0666; R(e) = 4.2155; R(f) = 1.0000; Pinva = 0.2800; Shape = 0.4692). Bayesian posterior probability values are given for appropriate clades. The newly generated sequence of the new species is in bold font.
Bayesian 50% majority rule consensus tree inferred from SSU rDNA of Filenchus multistriatus n. sp. under the GTR + G + I model (-ln L = 20913.9590; freq A = 0.2301; freqC = 0.2419; freqG = 0.3006; freq T = 0.2273; R(a) = 0.8974; R(b) = 2.1547; R(c) = 0.9066; R(d) = 1.0666; R(e) = 4.2155; R(f) = 1.0000; Pinva = 0.2800; Shape = 0.4692). Bayesian posterior probability values are given for appropriate clades. The newly generated sequence of the new species is in bold font.

Figure 7:

Bayesian 50% majority rule consensus tree inferred from LSU rDNA D2-D3 sequences of Filenchus multistriatus n. sp. under the GTR+G+I model (-ln L = 22199.4473; freq A = 0.1507; freqC = 0.2476; freqG = 0.3242; freq T = 0.2775; R(a) = 0.8948; R(b) = 0.8948; R(c) = 1.0152; R(d) = 0.7990; R(e) = 3.4497; R(f) = 1.0000; Pinva = 0.1528; Shape = 0.7240). Bayesian posterior probability values are given for appropriate clades. The newly generated sequences of the new species are in bold font.
Bayesian 50% majority rule consensus tree inferred from LSU rDNA D2-D3 sequences of Filenchus multistriatus n. sp. under the GTR+G+I model (-ln L = 22199.4473; freq A = 0.1507; freqC = 0.2476; freqG = 0.3242; freq T = 0.2775; R(a) = 0.8948; R(b) = 0.8948; R(c) = 1.0152; R(d) = 0.7990; R(e) = 3.4497; R(f) = 1.0000; Pinva = 0.1528; Shape = 0.7240). Bayesian posterior probability values are given for appropriate clades. The newly generated sequences of the new species are in bold font.

Morphometrics of Filenchus multistriatus n_ sp_ and F_ sandneri Wasilewska, 1965 from Bushehr province, Iran_ All measurements are in μm and in the form: mean ± SD (range)_

F. multistriatus n. sp.F. sandneri
CharacterHolotypeParatypesBushehr population
SexFemaleFemaleFemale
n1119
L567537.3±33.0 (500-590)400.1±48.0 (331-454)
L′497464.4±33.1 (423-515)352.8±46.0 (288-406)
a40.535.0±3.5 (29.3-40.5)31.1±4.0 (25.5-37.6)
b5.35.5±0.3 (5.0-6.0)5.1±0.5 (4.2-5.9)
c8.17.4±0.5 (6.4-8.4)8.4±0.8 (7.3-9.5)
c′7.07.2±0.9 (5.8-8.3)5.7±0.3 (5.3-6.5)
V70.569.6±3.0 (63.2-73.8)73.1±2.5 (68.1-75.8)
V′80.581.5±1.9 (77.8-84.9)83.7±1.8 (81.4-86.2)
Lip region height1.81.8±0.1 (1.7-1.9)2.1±0.3 (1.5-2.4)
Lip region width0.76.8±0.3 (6-7)5.0±0.2 (4.5-5.3)
Stylet6.67.1±0.4 (6.5-7.6)8.0±0.5 (7.5-8.6)
Conus2.12.5±0.4 (2-3)2.8±0.2 (2.6-3.0)
m31.835.5±3.5 (30.8-39.5)35.4±1.8 (32.5-38.5)
DGO0.70.7±0.1 (0.5-0.8)2.7±0.2 (2.5-3.0)
Excretory pore8175.4±5.5 (67-81)57.7±5.5 (50-64)
Median bulb4738.2±4.4 (35-47)--
MB43.938.7±3.9 (34.7-48.5)35.8±2.8 (30.5-41.3)
Pharynx10798.6±3.8 (94-107)78.1±5.9 (71-90)
Body width (BW)1415.5±2.0 (14-20)12.9±0.9 (12-14)
Anterior end to vulva400374±35 (316-427)292.8±40.3 (236-343)
Vulva - anus9785±11 (67-105)57.0±8.4 (40-70)
Anal body width1010.3±1.0 (9-12)8.3±0.5 (8-9)
PUS1212.2±0.7 (11-13)8.3±0.4 (7.8-8.8)
PUS/BW0.90.8±0.1 (0.6-0.9)8.3±0.4 (7.8-8.8)
Tail7072.9±3.1 (70-78)47.3±3.1 (43-52)
Tail/V-A0.70.9±0.1 (0.7-1.2)0.8±0.1 (0.6-1.2)
DOI: https://doi.org/10.2478/jofnem-2023-0008 | Journal eISSN: 2640-396X | Journal ISSN: 0022-300X
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Language: English
Submitted on: Jan 4, 2023
Published on: May 17, 2023
Published by: Society of Nematologists, Inc.
In partnership with: Paradigm Publishing Services
Publication frequency: 1 issue per year
Keywords:
LSU rDNA D2-D3,
New species,
SSU rDNA,
Taxonomy,
Tylenchinae
Related subjects:
Life sciences,
Life sciences, other

© 2023 Somayeh Monemi, Mohammad Reza Atighi, Joaquín Abolafia, Pablo Castillo, Majid Pedram, published by Society of Nematologists, Inc.
This work is licensed under the Creative Commons Attribution 4.0 License.

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