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Deladenus bonabensis n. sp. (Tylenchomorpha: Neotylenchidae) from East Azarbaijan province, northwestern Iran: A morphological and molecular phylogenetic study

Open Access
|Mar 2023

Figures & Tables

Figure 1

Line drawings of Deladenus bonabensis n. sp. A: Mycetophagous female; B: Mycetophagous male; C: Anterior body region, female; D: Posterior body region, female; D1: Tail tip, female; E: Posterior body region, male; F: Parts of reproductive system, female; G: Lateral lines, female.
Line drawings of Deladenus bonabensis n. sp. A: Mycetophagous female; B: Mycetophagous male; C: Anterior body region, female; D: Posterior body region, female; D1: Tail tip, female; E: Posterior body region, male; F: Parts of reproductive system, female; G: Lateral lines, female.

Figure 2

Light micrographs of Deladenus bonabensis n. sp. A: Anterior body region, female; B: Anterior body region, male; C: Part of pharynx, female, showing the position of secretory-excretory pore and hemizonid (upper arrow showng hemizonid, lower arrow showing the secretory-excretory pore); D: Lateral lines, female; E & F: Posterior body region, female; G: Posterior body region, male; H: Spicules and gubernaculum, male; H1: Penial tube, male. All scale bars = 10 μm.
Light micrographs of Deladenus bonabensis n. sp. A: Anterior body region, female; B: Anterior body region, male; C: Part of pharynx, female, showing the position of secretory-excretory pore and hemizonid (upper arrow showng hemizonid, lower arrow showing the secretory-excretory pore); D: Lateral lines, female; E & F: Posterior body region, female; G: Posterior body region, male; H: Spicules and gubernaculum, male; H1: Penial tube, male. All scale bars = 10 μm.

Figure 3

Bayesian 50% majority rule consensus tree of Deladenus bonabensis n. sp. based on small subunit (SSU) rDNA sequences under GTR + I + G model. Bayesian posterior probability values more than 0.50 are given for appropriate clades. The new sequence is indicated in bold.
Bayesian 50% majority rule consensus tree of Deladenus bonabensis n. sp. based on small subunit (SSU) rDNA sequences under GTR + I + G model. Bayesian posterior probability values more than 0.50 are given for appropriate clades. The new sequence is indicated in bold.

Figure 4

Bayesian 50% majority rule consensus tree of Deladenus bonabensis n. sp. based on large subunit (LSU) rDNA D2-D3 sequences under GTR + I + G model. Bayesian posterior probability values more than 0.50 are given for appropriate clades. The new sequence is indicated in bold.
Bayesian 50% majority rule consensus tree of Deladenus bonabensis n. sp. based on large subunit (LSU) rDNA D2-D3 sequences under GTR + I + G model. Bayesian posterior probability values more than 0.50 are given for appropriate clades. The new sequence is indicated in bold.

Morphometrics of Deladenus bonabensis n_ sp_ All measurements are in μm and in the form: mean ± standard deviation (range)_

CharactersHolotypeParatypes

FemaleFemalesMales
n198
L10851088 ± 45 (1051–1185)986 ± 32 (940–1039)
a42.742.7 ± 3.0 (39–48)46.5 ± 5.5 (38.5–57.5)
b9.09.0 ± 0.5 (8.0–9.5)8.8 ± 0.5 (8.5–9.5)
3.73.8 ± 0.2 (3.5–4.0)4.0 ± 0.3 (3.8–4.7)
c27.027.0 ± 2.5 (23.5–29.5)26.0 ± 4.0 (20.5–33.5)
2.32.5 ± 0.3 (2.0–3.0)2.5 ± 0.3 (2.2–3.0)
V or T94.594.2 ± 0.5 (93.5–95.0)52.5 ± 3.5 (48.5–58.5)
Lip region height22.3 ± 0.2 (2.0–2.5)2.5 ± 0.1 (2.2–2.5)
Lip region width at base7.57.3 ± 0.7 (6–8)7.0 ± 0.3 (6.5–7.5)
Lip region width at the apex5.75.5 ± 0.5 (5.0–6.5)5.5 ± 0.5 (5–6)
Max. body dim.2625.5 ± 2.0 (22–28)21.5 ± 3.0 (17–27)
Pharynx124123 ± 7 (114–135)112 ± 6 (101–122)
Anterior end to the end of pharyngeal glands272289 ± 18 (270–312)243 ± 21 (205–267)
Reproductive tract683655 ± 41 (574–705)517 ± 33 (488–561)
Anterior end to vulva10211025 ± 44 (982–1120)
Stylet total length99.5 ± 1.0 (8.5–11.0)9.5 ± 0.5 (9.0–10.5)
Stylet conus44.0 ± 0.5 (3.5–5.0)4.0 ± 0.5 (3.5–4.5)
Anterior end to DGO11.512.0 ± 1.0 (11.0–13.5)
Secretory-excretory pore from anterior end158163 ± 11 (147–181)149 ± 17 (134–185)
Nerve ring from anterior end112116 ± 7 (108–127)112 ± 14 (97–140)
Hemizonid from anterior end148148 ± 6 (140–159)137 ± 19 (119–178)
Vulval body diameter2725 ± 2 (21–28)
Anal body diameter1817.0 ± 1.5 (15–19)15.0 ± 1.5 (13–18)
Tail4040.5 ± 3.5 (36–46)39.0 ± 6.5 (30–51)
Spicules25.5 ± 1.5 (24–28)
Gubernaculum7.0 ± 0.5 (7–8)
DOI: https://doi.org/10.2478/jofnem-2023-0002 | Journal eISSN: 2640-396X | Journal ISSN: 0022-300X
Language: English
Submitted on: Sep 15, 2022
Published on: Mar 9, 2023
Published by: Society of Nematologists, Inc.
In partnership with: Paradigm Publishing Services
Publication frequency: 1 issue per year

© 2023 Mohammad Amiri Bonab, Mohammad Reza Atighi, Majid Pedram, published by Society of Nematologists, Inc.
This work is licensed under the Creative Commons Attribution 4.0 License.