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Morphological and Molecular Characterization of Tylenchorhynchus clarus Allen, 1955 and T. zeae Sethi & Swarup, 1968 (Rhabditida: Telotylenchidae) from Iraq Cover

Morphological and Molecular Characterization of Tylenchorhynchus clarus Allen, 1955 and T. zeae Sethi & Swarup, 1968 (Rhabditida: Telotylenchidae) from Iraq

Open Access
|Oct 2022

Figures & Tables

Figure 1

Light photomicrographs of Tylenchorhynchus clarus Allen, 1955 from Iraq. (A) Entire body; (B, C) Anterior body region; (D) Median bulb; (E, F) Terminal bulb (the arrow indicates the excretory pore); (G) Lateral field at mid-body; (H) Vulval region; (I–K) Posterior body region (the arrow indicates the phasmid). (Scale bars: A, 50 μm; B–K, 10 μm).
Light photomicrographs of Tylenchorhynchus clarus Allen, 1955 from Iraq. (A) Entire body; (B, C) Anterior body region; (D) Median bulb; (E, F) Terminal bulb (the arrow indicates the excretory pore); (G) Lateral field at mid-body; (H) Vulval region; (I–K) Posterior body region (the arrow indicates the phasmid). (Scale bars: A, 50 μm; B–K, 10 μm).

Figure 2

Light photomicrographs of Tylenchorhynchus zeae Sethi and Swarup, 1968 from Iraq. (A, C–K) Female. (A) Entire body; (C, D) Anterior body region; (E) Median bulb and isthmus; (F, G) Terminal bulb (the arrow indicates the excretory pore); (H) Lateral field at mid-body; (I) Vulval region; (J, K) Posterior body region (the arrow indicates the phasmid); (B, L, M) Male. (B) Entire body; (L, M) Posterior body region. (Scale bars: A and B, 50 μm; C–M, 10 μm).
Light photomicrographs of Tylenchorhynchus zeae Sethi and Swarup, 1968 from Iraq. (A, C–K) Female. (A) Entire body; (C, D) Anterior body region; (E) Median bulb and isthmus; (F, G) Terminal bulb (the arrow indicates the excretory pore); (H) Lateral field at mid-body; (I) Vulval region; (J, K) Posterior body region (the arrow indicates the phasmid); (B, L, M) Male. (B) Entire body; (L, M) Posterior body region. (Scale bars: A and B, 50 μm; C–M, 10 μm).

Figure 3

Bayesian 50% majority rule consensus tree inferred from analysis of the D2–D3 domains of the LSU rDNA sequences of Iraqi populations of Tylenchorhynchus clarus Allen, 1955 and T. zeae Sethi and Swarup, 1968 under the GTR + G + I model. Bayesian posterior probability values of >0.50 are given for appropriate clades. New sequences are indicated in bold.
Bayesian 50% majority rule consensus tree inferred from analysis of the D2–D3 domains of the LSU rDNA sequences of Iraqi populations of Tylenchorhynchus clarus Allen, 1955 and T. zeae Sethi and Swarup, 1968 under the GTR + G + I model. Bayesian posterior probability values of >0.50 are given for appropriate clades. New sequences are indicated in bold.

Figure 4

Bayesian 50% majority rule consensus tree inferred from analysis of the ITS rDNA of Iraqi populations of Tylenchorhynchus clarus Allen, 1950 and T. zeae Sethi and Swarup, 1968 under the GTR + G + I model. Bayesian posterior probability values of >0.50 are given for appropriate clades. New sequences are indicated in bold.
Bayesian 50% majority rule consensus tree inferred from analysis of the ITS rDNA of Iraqi populations of Tylenchorhynchus clarus Allen, 1950 and T. zeae Sethi and Swarup, 1968 under the GTR + G + I model. Bayesian posterior probability values of >0.50 are given for appropriate clades. New sequences are indicated in bold.

Morphometrics of Tylenchorhynchus clarus Allen, 1955 and T_ zeae Sethi and Swarup, 1968 from Misan province, Iraq_ All measurements are in micrometer and in the form: mean ± s_d_ (range)_

CharactersTylenchorhynchus clarusTylenchorhynchus zeae
FemalesFemalesMales
n14135
L459.1 ± 34.9 (414–523)534.2 ± 15.5 (501–570)483.5 ± 23.9 (455–521)
a28.4 ± 1.2 (25.7–29.7)28.9 ± 0.7 (25.7–32.2)31.2 ± 0.4 (30.5–31.9)
b4.6 ± 0.4 (4.0–5.4)4.7 ± 0.1 (4.6–5.0)4.7 ± 0.3 (4.3–5.2)
c16.4 ± 1.5 (14.6–19.4)16.7 ± 2.7 (14.0–20.4)16.9 ± 2.1 (14.8–18.2)
c'2.5 ± 0.2 (2.1–2.8)2.2 ± 0.3 (1.9–2.6)2.2 ± 0.2 (2.1–2.4)
V57.5 ± 3.6 (53.5–59.8)57.4 ± 3.7 (55.4–61.9)
Lip region height3.1 ± 0.2 (2.8–3.2)3.0 ± 0.2 (2.8–3.2)2.7 ± 0.4 (2.4–3.3)
Lip region width6.6 ± 0.4 (6.4–7.2)5.7 ± 0.7 (5.5–7.3)5.4 ± 0.4 (4.9–6.0)
DGO1.9 ± 0.2 (1.3–2.4)3.1 ± 0.1 (2.9–3.3)3.1 ± 0.1 (2.9–3.3)
MB51.2 ± 1.4 (46.6–55.8)54.5 ± 1.9 (48.1–56.2)53.3 ± 0.9 (52.2–54.7)
Stylet length16.8 ± 1.6 (15.4–18.9)18.2 ± 0.8 (16.7–19.2)16.9 ± 0.6 (15.8–18.2)
Pharynx length105.2 ± 4.8 (98–120)113.3 ± 4 (106–117)100.9 ± 6.4 (91–107)
Anterior end to excretory pore76.9 ± 2.8 (72.8–86.8)88.1 ± 3.2 (82–92)76.2 ± 7.3 (70.5–87.1)
Maximum body width16.2 ± 0.9 (15.2–17.6)16.2 ± 0.4 (15.7–16.6)15.4 ± 0.7 (14.9–16.6)
Anal body width11.4 ± 0.9 (10.4–13.6)12.4 ± 0.4 (11.6–14.2)9.6 ± 0.9 (9.1–10.7)
Vulval body width15.5 ± 0.9 (14.4–17.8)17.5 ± 1.2 (14.6–19.2)
Tail length29.1 ± 1.6 (27.5–33.2)30.8 ± 2.9 (25.2–38.2)26.8 ± 2.6 (24.2–33.7)
Tail annuli15.5 ± 1.3 (14–16)17.5 ± 1.1 (16–21)
Spicule length17.5 ± 0.2 (16.1–18.9)
Gubernaculum7.6 ± 0.3 (7.3–9.1)
DOI: https://doi.org/10.2478/jofnem-2022-0043 | Journal eISSN: 2640-396X | Journal ISSN: 0022-300X
Language: English
Submitted on: Jun 3, 2022
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Published on: Oct 19, 2022
In partnership with: Paradigm Publishing Services
Publication frequency: 1 issue per year

© 2022 Ahmed Malik Jumaah, Sedighe Azimi, published by Society of Nematologists, Inc.
This work is licensed under the Creative Commons Attribution 4.0 License.